Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Ectopic expression of $ARR1{\Delta}DDK$ in tobacco: alteration of cell fate in root tip region and shoot organogenesis in cultured segments

  • Rashid, Syeda Zinia (Department of Life Sciences, Faculty of Agriculture, Kagawa University) ;
  • Kyo, Masaharu (Department of Life Sciences, Faculty of Agriculture, Kagawa University)
  • Received : 2009.09.04
  • Accepted : 2009.11.18
  • Published : 2010.03.30
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Abstract

A specific deleted version of ARABIDOPSIS RESPONSE REGULATOR1 (ARR1) lacking the signal receiver domain (1.152 amino acids)-coding sequence, referred to as $ARR1{\Delta}DDK$, was amplified using Arabidopsis thaliana cDNA prepared from adult leaves and transferred into the genome of Nicotiana tabacum cv. Samsun under the transcriptional control of a ${\beta}$-estradiol-inducible expression system. The ectopic expression of $ARR1{\Delta}DDK$ affected the morphology of transgenic seedlings and their segments in vitro. In the presence of an inducer, ${\beta}$-estradiol, ectopic expression of $ARR1{\Delta}DDK$ induced only the formation of soft, pseudo-bulbous tissue in the root tip region of intact seedlings, which appeared similar to callus generated on a hypocotyl segment in the presence of 2,4-D and 6-benzyladenine (BA), both at $1\;{\mu}M$. Those callus tissues on the root tip region could not generate shoots unless $1\;{\mu}M$ BA was supplied. In segment culture, ectopic expression of $ARR1{\Delta}DDK$ induced calluslike tissue around the cut-end of cotyledon and hypocotyl segments with occasional shoot formation, suggesting that the expression of $ARR1{\Delta}DDK$ could substitute for the effects of cytokinin on these segments. Additionally, treatment with only ${\beta}$-estradiol induced NtWUS, a WUS ortholog in tobacco, which was detected during the process of callus tissue formation in the root tip region and also in cotyledon or hypocotyl segments. These findings suggest that the NtWUS might be associated in the transdifferentiation process caused by the functional regulation of $ARR1{\Delta}DDK$ in transgenic tobacco seedlings.

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References

  1. Brandstatter I, Keiber JJ (1998) Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis. Plant Cell 10:1009-1019 https://doi.org/10.1105/tpc.10.6.1009
  2. D'Agostino IB, Kieber JJ (1999) Phosphorelay signal transduction: the emerging family of plant response regulators. Trends Biochem Sci 24:452-456 https://doi.org/10.1016/S0968-0004(99)01465-6
  3. D'Agostino IB, Deruere J, Kieber JJ (2000) Characterization of the response of the Arabidopsis response regulator gene family to cytokinin. Plant Physiol 124:1706-1717 https://doi.org/10.1104/pp.124.4.1706
  4. Ferreira FJ, Kieber J (2005) Cytokinin signaling. Curr Opin Plant Biol 8:518-525 https://doi.org/10.1016/j.pbi.2005.07.013
  5. Higuchi M, Pischke MS, Mahonen AP et al (2004) In planta functions of the Arabidopsis cytokinin receptor family. Proc Natl Acad Sci USA 101:8821-8826 https://doi.org/10.1073/pnas.0402887101
  6. Hwang I, Sheen J (2001) Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature 413:383-389 https://doi.org/10.1038/35096500
  7. Inoue T, Higuchi M, Hashimoto Y, Seki M, Kobayashi M, Kato T, Tabata S, Shinozaki K, Kakimoto T (2001) Identification of CRE as a cytokinin receptor from Arabidopsis. Nature 409:1060-1063 https://doi.org/10.1038/35059117
  8. Kakimoto T (1996) CKI1, a histidine kinase homolog implicated in cytokinin signal transduction. Science 274:982-985 https://doi.org/10.1126/science.274.5289.982
  9. Leibfried A, To JP, Busch W, Stehling S, Kehle A, Demar M, Kieber JJ, Lohman JU (2005) WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators. Nature 438:1172-1175 https://doi.org/10.1038/nature04270
  10. Linsmaier EM, Skoog F (1965) Organic growth factor requirements in tobacco tissue cultures. Physiol Plant 18:100-128 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
  11. Mason MG, Mathews DE, Argyros DA, Maxwell BB, Kieber JJ, Alonso JM, Ecker JR, Schaller GE (2005) Multiple type-B response regulators mediate cytokinin signal transduction in Arabidopsis. Plant Cell 17:3007-3018 https://doi.org/10.1105/tpc.105.035451
  12. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor
  13. Mok DW, Mok MC (2001) Cytokinin metabolism and action. Annu Rev Plant Physiol Plant Mol Biol 52:89-118 https://doi.org/10.1146/annurev.arplant.52.1.89
  14. Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C (2004) Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis. Plant Cell 16:1365-1377 https://doi.org/10.1105/tpc.021477
  15. Rashid SZ, Kyo M (2009a) Ectopic expression of WOX5 dramatically alters root-tip morphology in transgenic tobacco. Transgenic Plant J (in press)
  16. Rashid SZ, Kyo M (2009b) In vivo functional assay of WUS ortholog in tobacco. Acta Hortic 829:161-166
  17. Rashid SZ, Yamaji N, Kyo M (2007) Shoot formation from root tip region: a developmental alteration by WUS in transgenic tobacco. Plant Cell Rep 26:1449-1455 https://doi.org/10.1007/s00299-007-0342-7
  18. Riefler M, Novak O, Strnad M, Schmulling T (2006) Arabidopsis cytokinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root development, and cytokinin metabolism. Plant Cell 18:40-54 https://doi.org/10.1105/tpc.105.037796
  19. Riou-Khamilichi C, Huntley R, Jackmard A, Murray JAH (1999) Cytokinin activation of Arabidopsis cell division through a D-type cyclin. Science 283:1541-1544 https://doi.org/10.1126/science.283.5407.1541
  20. Sakai H, Aoyama T, Oka A (2000) Arabidopsis ARR1 and ARR2 response regulators operate as transcriptional activators. Plant J 26:703-711
  21. Sakai H, Honma T, Aoyama T, Sato S, Kato T, Tabata S, Oka A (2001) ARR1, a transcription factor for genes immediately responsive to cytokinins. Science 294:1519-1521 https://doi.org/10.1126/science.1065201
  22. Sakakibara H, Suzuki M, Takei K, Deji A, Taniguchi M, Sugiyama T (1998) A response-regulator homologue possibly involved in nitrogen signal transduction mediated by cytokinin in maize. Plant J 14:337-344 https://doi.org/10.1046/j.1365-313X.1998.00134.x
  23. Tamaoki M, Kusaba S, Kano-Murakami Y, Matsuoka M (1997) Ectopic expression of a tobacco homeobox gene NTH15, dramatically alters leaf morphology and hormone levels in transgenic tobacco. Plant Cell Physiol 38:917-927 https://doi.org/10.1093/oxfordjournals.pcp.a029252
  24. To JPC, Kieber JJ (2008) Cytokinin signaling: two components and more. Trends Plant Sci 13(2):85-92 https://doi.org/10.1016/j.tplants.2007.11.005
  25. Ueguchi C, Koizumi H, Suzuki T, Mizuno T (2001) Novel family of sensor histidine kinase genes in Arabidopsis thaliana. Plant Cell Physiol 42:231-235 https://doi.org/10.1093/pcp/pce015
  26. Werner T, Motyka V, Laucou V, Smets R, Van Onckelen HV, Schmulling T (2003) Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. Plant Cell 15:2532-2550 https://doi.org/10.1105/tpc.014928
  27. Zuo J, Niu Q-W, Chua N-H (2000) An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J 24:265-273 https://doi.org/10.1046/j.1365-313x.2000.00868.x
  28. Zuo J, Niu Q-W, Frugis G, Chua N-H (2002) The WUSCHEL gene promotes vegetative-to-embryonic transition in Arabidopsis. Plant J 30:349-359 https://doi.org/10.1046/j.1365-313X.2002.01289.x

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