[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.1007/s10059-009-0117-9

Expression of BrD1, a Plant Defensin from Brassica rapa, Confers Resistance against Brown Planthopper (Nilaparvata lugens) in Transgenic Rices

Choi, Man-Soo (National Institute of Crop Science, Rural Development Administration)
Kim, Yul-Ho (National Institute of Crop Science, Rural Development Administration)
Park, Hyang-Mi (National Institute of Crop Science, Rural Development Administration)
Seo, Bo-Yoon (National Institute of Crop Science, Rural Development Administration)
Jung, Jin-Kyo (National Institute of Crop Science, Rural Development Administration)
Kim, Sun-Tae (Department of Plant Bioscience, Pusan National University)
Kim, Min-Chul (Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
Shin, Dong-Bum (National Institute of Crop Science, Rural Development Administration)
Yun, Hong-Tai (National Institute of Crop Science, Rural Development Administration)
Choi, Im-Soo (National Institute of Crop Science, Rural Development Administration)
Kim, Chung-Kon (National Institute of Crop Science, Rural Development Administration)
Lee, Jang-Yong (National Academy of Agricultural Science, Rural Development Administration)

Abstract

Plant defensins are small (5-10 kDa) basic peptides thought to be an important component of the defense pathway against fungal and/or bacterial pathogens. To understand the role of plant defensins in protecting plants against the brown planthopper, a type of insect herbivore, we isolated the Brassica rapa Defensin 1 (BrD1) gene and introduced it into rice (Oryza sativa L.) to produce stable transgenic plants. The BrD1 protein is homologous to other plant defensins and contains both an N-terminal endoplasmic reticulum signal sequence and a defensin domain, which are highly conserved in all plant defensins. Based on a phylogenetic analysis of the defensin domain of various plant defensins, we established that BrD1 belongs to a distinct subgroup of plant defensins. Relative to the wild type, transgenic rices expressing BrD1 exhibit strong resistance to brown planthopper nymphs and female adults. These results suggest that BrD1 exhibits insecticidal activity, and might be useful for developing cereal crop plants resistant to sap-sucking insects, such as the brown planthopper.

Keywords

Brassica rapa; brown planthopper (BPH); Oryza sativa L.; plant defensin; resistance; transgenic plant;

Citations & Related Records

Times Cited By KSCI : 3 (Citation Analysis)
Times Cited By Web Of Science : 3 (Related Records In Web of Science)

Reference
Cited By KSCI

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