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http://dx.doi.org/10.5010/JPB.2012.39.4.273

Comparison of transcriptome analysis between red flash peach cultivar and white flash peach cultivar using next generation sequencing  

Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Hwang, Hae Seong (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Journal of Plant Biotechnology / v.39, no.4, 2012 , pp. 273-280 More about this Journal
Abstract
Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress, ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.
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