• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.55-55
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• 2019

Strawberry which is the genus Fragaria under family Rosaceae is one of the most important fruit plants for both fresh consumption and food processing in the temperate and subtropical countries. Propagation of strawberry is achieved either through runners or by in vitro micropropagation. Meristem tips, generally obtained from runners of virus-free plants, are commonly used to establish in vitro cultures, which are employed for mass propagation or as a source of plant material for regeneration and transformation experiments. This study was conducted to determine the optimal natural additives strength to improve sprouting shoot rate of apical meristem of strawberry 'Seolhyang'. Strawberry apical meristem at size (0.2 mm to 0.3 mm) with leaf primordials were cultured on the 1/3MS(Murashige & Skoog) medium supplemented with five natural additives such as coconut milk, maple sap, banana powder and peptone. The sprouting ratio and growth characteristics were evaluated after eight weeks after in vitro culture. Shoot ratio of 'Seolhyang' apical meristem was 72.9% in 1/3MS medium supplemented with maple sap. On the other hand, the low shoot ratio was observed 47.7% in 1/3MS medium supplemented with banana powder. Shoot length was different as natural additives but numbers of leaf was not significantiy different among the natural additives. As a result, the sprouting ratio and plant growth were enhanced effectively in 1/3MS medium with maple sap compared to the others.

• Current Research on Agriculture and Life Sciences
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• 제18권
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• pp.1-7
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• 2000

본 실험은 식물조직배양 기법을 통하여 한란과 심비디움의 효율적인 증식을 위한 식물 생장조절물질의 적정 처리방법을 구명하고자 수행하였다. 한란의 rhizome 형성은 무처리 및 10 ppm kinetin + 2 ppm NAA, 심비디움의 protocorm 형성은 무처리 및 10 ppm kinetin + 0.05 ppm NAA에서 아주 양호하였다. Callus 유도의 적정 식물 생장조절물질의 농도는 구명하지 못했다. 한란 rhizome으로부터의 shoot 분화에는 10 ppm BA + 2 ppm NAA, 5 ppm BA + 2ppm NAA, 심비디움 protocorm으로부터의 shoot 분화는 10 ppm Kinetin + 0.05 ppm NAA, 1 ppm Kinetin + 2 ppm NAA에서 비교적 효과적인 것으로 나타났다.

• 한국약용작물학회지
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• 제8권2호
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• pp.123-128
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• 2000

지황 기내 식물체의 줄기 조직을 재료로 생물반응기에서 부정아 발생 과정을 통하여 기내 종묘를 생산하기 위해 본 실험을 수행하였다. 공기부양형 생물반응기가 교반형에 생물반응기의비해 신초 형성에 유리하였다. 2.5L 규모의 생물반응기에서 적절한 배양 밀도는 배지 1.5L에 시료 50g(줄기절편 90개)이었고, 이 때 공기의 주입량은 0.5 v.v.m으로 조절하는 것이 효과적이었다. 신초의 생산 효율을 높이기 위해 배지에 pH 완충제인 MES를 첨가한 결과, 생성된 신초의 수가 증가하였다. 유리화 억제제를 5g/1의 농도로 배지에 첨가하였을 경우 신초의 유리화 현상이 뚜렷하게 억제됨과 동시에 신초의 형성도 증가되었다.

• Journal of Plant Biotechnology
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• 제31권1호
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• pp.61-65
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• 2004

본 실험은 Alocasia cadieri Chantrier를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Alocasia의 경정을 TDZ 0.1mg/L가 첨가된 배지에 배양하였을 때 신초증식이 가장 높았으며, 형성된 신초에서 신초의 증식은 TDZ과 NAA가 각각 0.5mg/L첨가된 배지에서 효과적이었다. 형성된 신초의 발근은 IBA또는 NAA가 첨가된 배지보다는 활성탄 2.0g/L가 첨가된 배지에서 양호하였으며, 소식물체의 순화는 perlite : vermiculite가 1:1로 혼합된 용토 또는 vermiculite가 적합하였다.

• 한국약용작물학회지
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• 제11권5호
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• pp.397-401
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• 2003

심장질환 및 암치료에 약제로 사용되어지는 D. purpurea의 잎 절편으로부터 신초의 재분화와 증식을 시도하였다. MS 배지에 $2\;mg/l$ BA와 $0.1\;mg/l IAA를 조합하여 처리하였을 때 잎절편으로부터 부정아가 형성되었다. 유기된 부정아로부터 신초의 증식은 식물생장조절물질이 첨가된 $B_5$ 배지에서 배양 5주 후 약 21개의 신초가 분화되었으며, 신초의 길이 신장과 생육은 WPM 배지와 MS 배지에서 가장 양호한 것으로 나타났다. 한편 기내 증식된 신초로 부터의 발근은 $1\;mg/l$ IBA를 첨가된 MS 배지에서 신초당 21.5개가 형성 되었다.

• 식물조직배양학회지
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• 제25권1호
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• pp.63-68
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• 1998

정단 및 마디조직을 이용한 지황의 기내증식방안을 마련하기 위하여 일련의 시험을 실시하였다. 재생된 신초의 정단을 재배양하였을 경우 BA 5.0 mg/L에서 7.8개의 신초가 형성되었으며 투명화된 묘의 발생은 Gelrite 보다는 Bacto-agar 첨가구에서 감소하였다. 광도와 한천의 농도가 증가할수록 신초형성수 및 생장은 억제되었고 생체중 : 건물중의 비율도 감소하였다. 광도가 2000 lx일 때는 Bacto-agar 0.6-1.2%, 3,000 lx에서는 한천 0.4-0.6%에서 건전한 신초를 10개 이상 생산할 수 있었다. 활성탄소 0.1-0.3%첨가는 신초의 생장촉진과 뿌리형성에 촉진적으로 작용하였으며 투명화 발생률도 감소시켰으나 신초형성수는 현저히 감소시켰다. MS배지내 첨가되는 다량원소의 농도를 0.8-1.0배 하였을때 신초형성수 및 생장이 촉진되었다. 기관형성에 미치는 오옥신의 종류 및 농도별 효과를 조사한 결과 NAA나 IBA 보다는 IAA 0.3mg/L 와 BA 5.0mg/L를 혼합처리 하였을 때 증식률이 16배에 달하였다.

• 한국자원식물학회지
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• 제7권1호
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• pp.17-22
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• 1994

This study was carried out to investigate the optimum medium and concentrations of growth regulators for induction of multiple shoot by meristem culture of floe otorefcenf Mill. MS medium supple-mented with 3${\mu}{\textrm}{m}$ TDZ was effective for induction of multiple shoot. Shoot multiplication was more ef-fective when 2mg/1 BA combined with 0.Img/1 IAA than when only BA were treated on medium. Halfstrength of MS medium supplemented with 2mg/L IAA was effective for rooting of shoots regenerated.When plantlets regenerated from meristem culture were transferred to pot, survival rate of plantletswas 80% on perlite and was 95% on vermiculite, respectively.

• 한국자원식물학회지
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• 제11권3호
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• pp.353-357
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• 1998

This study was conducted to determine the effect of growth regulators, genotype, and cutting position on the rooting and root growth from cutting of Chrysanthemum zawadskii H.. Rooting rate of Keungugeolcho in the treatement of IBA 500 and 1000 ppm was the better than those of other treatments of IAA, NAA and Rooton. Rooting rate differed depending on the genotype. Hangryobonggugeolcho was better than Keungucheolcho in rooting rate. The treatment of rooton remarkably induced many roots from the cuttings of eight accessions of Chrysanthemum zawadskii H.. Also, rooting rate and number of root differed depending on cutting position. When cuttings including shoot tip were cultured on tray containing bed soil, rooting rate and number of root induced from cuttings with shoot tip was higher than when cuttings without shoot tip and with lateral axillary bud were cultured.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.15-15
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• 2019

The walnut (Juglans regia L.), a member of the Juglandaceae, is native to the mountain ranges of central Asia. This species of walnut is valued commercially for its nuts and in some areas for its timber. The seeds of walnut are recalcitrant and it has strong integument dormancy and their germination is irregular, making its natural propagation difficult. Low percentage of seed germination and long propagation cycle are the main problems of propagation. This study was conducted medium composition on in vitro plantlet regeneration from axillary buds of walnut. It has proved to be the most generally applicable and reliable method of in vitro propagation. Micropropagation culture that axillary buds are excised aseptically enables faster multiplication of plants. The axillary buds of walnut new cultivar "Sinlyeong" were cultured on two basal media which contained the different plant growth regulators depending on the respective shooting and rooting stage. After 12 weeks, the shoot generation rate was 85.3%, the shoot number and its length were 1.9/explant and 2.7 cm in the most favorable medium composition. The percentage of rooting was 25.4%. From these results, it was found the optimum basal medium and plant growth regulator for in vitro plant regeneration from axillary buds of walnut new cultivar "Sinlyeong". However, we have continued to search the other medium additives to enhance the rate of walnut root.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.85-94
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• 2010

In vitro shoot regeneration of gladiolus in three different culture systems, viz., semi-solid agar (AS), membrane raft (MR), and duroplast foam liquid (DF) cultures was evaluated following the kinetics of shoot multiplication and hyperhydricity at optimized growth regulator combinations. Compared to the AS system, matrixsupported liquid cultures enhanced shoot multiplication. The peak of shoot multiplication rate was attained at 18 days of incubation in the MR and DF systems, whereas the maximum rate in the AS system was attained at 21 days. An early decline in acceleration trend was observed in liquid cultures than the AS culture. The hyperhydric status of the regenerated shoots in the different culture systems was assessed in terms of stomatal attributes and antioxidative status. Stomatal behavior appeared to be normal in the AS and MR systems. However, structural anomaly of stomata such as large, round shaped guard cells with damage in bordering regions of stomatal pores was pronounced in the DF system along with a relatively higher $K^+$ ion concentration than in the AS and MR systems. Antioxidative status of regenerated shoots was comparable in the AS and MR systems, while a higher incidence of oxidative damages of lipid membrane as evidenced from malondialdehyde and ascorbate content was observed in the DF system. Higher oxidative stress in the DF system was also apparent by elevated activities of superoxide dismutase, ascorbate peroxidase, and catalase. Among the three culture systems, liquid culture with MR resulted in maximum shoot multiplication with little or no symptoms of hyperhydricity. Shoots in the DF system were more prone to hyperhydricity than those in the AS and MR systems. The use of matrix support such as membrane raft as an interface between liquid medium and propagating tissue could be an effective means for rapid and efficient mass propagation with little or no symptoms of hyperhydricity.