Current Research on Agriculture and Life Sciences

  • Volume 18
  • /
  • Pages.1-7
  • /
  • 2000
  • /
  • 2287-271X(pISSN)
  • /
  • 2288-0356(eISSN)
Institute of Agricultural Science and Technology, Kyungpook National University (경북대학교 농업과학기술연구소)
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Effects of Plant Growth Regulators on in vitro Propagation of Cymbidium kanran and Cymbidium hybrida

한란 및 심비디움의 기내 증식에 미치는 생장조절물질의 효과

  • Kim, Hak-Yoon (Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • Kwon, Soom-Tae (Dept. of Horticulture and breeding, Andong National University)
  • 김학윤 (경북대학교 농업과학기술연구소) ;
  • 권순태 (안동대학교 자연과학대학 원예육종학과)
  • Published : 2000.12.31
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Abstract

This study was carried out to determine the effects of plant growth regulators on organogenesis from Cymbidium kanran and Cymbidium hybrida. Optimal rhizome formation from Cymbidium kanran was obtained on MS medium with 10 ppm kinetin+2 ppm NAA. and optimal protocorm formation from Cymbidium hybrida was obtained on MS medium with 10 ppm kinetin+0.05 ppm NAA. However, in this study the optimal media for the callus induction from both explants was not identified. Optimal shoot induction from rhizome of Cymbidium kanran was obtained on MS medium with 10 ppm BA+2 ppm NAA and 5 ppm BA+2 ppm NAA. Optimal shoot induction from protocorm of Cymbidium hybrida was obtained on MS medium with 10 ppm kinetin+2 ppm NAA.

본 실험은 식물조직배양 기법을 통하여 한란과 심비디움의 효율적인 증식을 위한 식물 생장조절물질의 적정 처리방법을 구명하고자 수행하였다. 한란의 rhizome 형성은 무처리 및 10 ppm kinetin + 2 ppm NAA, 심비디움의 protocorm 형성은 무처리 및 10 ppm kinetin + 0.05 ppm NAA에서 아주 양호하였다. Callus 유도의 적정 식물 생장조절물질의 농도는 구명하지 못했다. 한란 rhizome으로부터의 shoot 분화에는 10 ppm BA + 2 ppm NAA, 5 ppm BA + 2ppm NAA, 심비디움 protocorm으로부터의 shoot 분화는 10 ppm Kinetin + 0.05 ppm NAA, 1 ppm Kinetin + 2 ppm NAA에서 비교적 효과적인 것으로 나타났다.

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