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Shoot Regeneration from Leaf Explants of Digitalis purpurea L.  

Hwang, Sung-Jin (Department of Food and Biotechnology, Dongshin University)
Lee, Hye-Jung (Department of Biology, Chonnam National University)
Hwang, Baik (Department of Biology, Chonnam National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.11, no.5, 2003 , pp. 397-401 More about this Journal
Abstract
Digitalis purpurea L. is a medicinal herb and have been used to congestive heart failure, mycocardial infarction, edema, angina etc. A protocol has been developed for in vitro propagation of adventitious shoot buds directly from leaf segments of D. purpurea Leaf explants of D. purpurea directly formed shoot buds when cultured on a MS medium supplemented with $2\;mg/l$ BA and $0.1\;mg/l$ IAA for 5 weeks. Adventitious shoots were multiplied by subculturing on the $B_5$ medium and shoot elongation was developed by subculturing on the WPM medium. Root formation from the shoot regenerated was achieved on MS basal medium containing 1 mg/ IBA.
Keywords
Digitalis purpurea; leaf explants; multiple shoots; rooting; plantlets;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Furuya T, Kojima H, Katsuta T (1972) 3-Methylpurpurin and other anthraquinones from callus tissues of Digitalis lanta. Phytochemistry 11:1073-1076   DOI   ScienceOn
2 Gavidia I, Perez-Bermudez P (1997) Cardenolides of Digitalis obscure: The effect of phosphate and manganese on growth and productivity of shoot-tip cultures. Phytochemistry 45:81-85   DOI   ScienceOn
3 Kayo Ideda, Daisuke Teshima, Toshinobu Aoyama, Motoyoshi Satake, and Koichiro Shimomura (1988) Clonal propagation of Cephaelis ipecacuanha. Plant Cell Rep. 7:288-291   DOI   ScienceOn
4 Lee MY, Yoon ES, Jung SJ, Bae KH, Seo JW, Choi YE (2002) Plant regeneration and effect of auxin and cytokinin on adventitious shoot formation from seedling explant of Taraxacum platycarpum. Korean J. Plant Biotech. 29:111-115   DOI
5 Pilgrim H (1977) Saponin in suspension cultures of Digitalis purpurea L. Phytochemistry 16:1311-1312   DOI   ScienceOn
6 李昌福 (1985) 大韓植物圖鑑. 鄕文社. P. 680
7 Kuberski C, Scheibner H, Steup C, Diettrich B, Luckner M (1984) Embryogenesis and cardenolide formation in tissue cultures of Digitalis lanta. Phytochemistry 23:1407-1412   DOI   ScienceOn
8 Masumoto M, Shoyama Y, Nishioka I (1988) Effect of bacterial and virus infection on iridoid glycoside contents in Rehmannia glutinosa Libosch. var purpurea Makino. Shoyakugaku Zasshi, 42:329-332
9 Mandal AKA, Gupta SD (2001) Direct shoot organogenesis and plant regeneration in safflower. In vitro Cell. Dev, Biol.- Plant. 37:50-54   DOI   ScienceOn
10 Pal BH, Green JB, Walker TS, Okemo PO, Vivanco JM (2002) In vitro propagation of Spilanthes mauritiana DC., an endangered medicinal herb, through axillary bud cultures. In vitro Cell. Dev. Biol.-Plant. 38:598-601   DOI   ScienceOn
11 Jiang LC, Mao WY (1979) Callus formation and plantlet regeneration of Rehmannia glutinosa. Chin. Med. Herb. Lett. 2:42
12 Park CH, Seong NS, Park KY, Lee CH (1988) Micropropagation through callus culture in Chinese foxglove (Rehmannia glutinose), Korean J. Plant Tissue Cult. 25:171-175
13 Imre S, Sar S, Thomson RH (1976) Anthraquinones in Digitalis species. Phytochemistry 15:317-320   DOI   ScienceOn
14 Furuya T, Kojima H (1971) 4-Hydroxydigitolutein, a new anthraquinone from callus tissue of Digitalis lanta. Phytochemistry 10:1607-1610   DOI   ScienceOn
15 Rha ES, Kim JK (1996) Plant regeneration in leaf explant cultures of Rehmannia glutinosa. Korewan J. Plant Tissue Cult. 23:299-302
16 李正日 , 桂風明 (1994) 藥用植物의 利用과 新栽培技術. 先進文化社 p. 147
17 Kim HK, Hong HJ, Kim DI (1994) Digoxin production by using biotransformation in Digitalis lanata cell suspension cultures. Korean J. Appl. Microbial. Biotechnol. 22:651-658
18 Perez-Bermudez P, Cornejo MJ, Segura J (1983) In vitro propagation of Digitalis obscura L. Plant Sci. Lett. 30:77-82   DOI   ScienceOn
19 Seong NS, Lee ST, Park KY, Chae YA, Park CH, Park SI (1993) Micropropagation of Aconitum carmichaeli and Rehmannia glutinosa through the plant tissue culture techniques. RDA Special Res. Rep. p. 46
20 Chengalrayan K, Mhaske VB, Hazra S (1998) Genotype control of peanut somatic embryogenesis. Plant Cell Rep. 17:522-525   DOI   ScienceOn
21 Harn CY (1984) Fundamental studies on plant breeding. RDA Res. Bull. p. 245-356
22 Ravishankar RV (2002) Rapid clonal propagation of Nothapodytes foetida (Wight)-A threatened medicinal tree. In vitro Cell. Dev. Biol.-Plant 38:347-351   DOI   ScienceOn
23 Masumoto M, Koga S, Shoyama Y, Nishioka I (1987) Phenolic glicoside composition of leaves and callus cultures of Digitalis purpurea L. Phytochemistry 26:3225-3227   DOI   ScienceOn
24 Tripepi RR (1977) Advantitious shoot regeneration. p. 45-71. In Geneve RL, Preece JE, Merkle SA (eds). Biotechnology of ornamental Plants.Wallingford, UK
25 Nand L, Paramvir SA (1989) Propagation of Indian rhubarh (Rheum emodi Wall.) using shoot-tip and leaf explant culture. Plant Cell Rep. 8:493-496   DOI   ScienceOn
26 Eisenbei M, Kreis W, Reinhard E (1999) Cardenolide biosynthesis in light and dark grown Digitalis lanta shoot cultures. Plant Physiol. Biochem. 37:13-23   DOI   ScienceOn