• Title/Summary/Keyword: shift operator

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A Point Mutation at the C-Terminal Half of the Repressor of Temperate Mycobacteriophage L1 Affects Its Binding to the Operator DNA

  • Ganguly, Tridib;Chattoraj, Partho;Das, Malabika;Chanda, Palas K.;Mandal, Nitai.C.;Lee, Chia Y.;Sau, Subrata
    • BMB Reports
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    • v.37 no.6
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    • pp.709-714
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    • 2004
  • The wild-type repressor CI of temperate mycobacteriophage L1 and the temperature-sensitive (ts) repressor CIts391 of a mutant L1 phage, L1cIts391, have been separately overexpressed in E. coli. Both these repressors were observed to specifically bind with the same cognate operator DNA. The operator-binding activity of CIts391 was shown to differ significantly than that of the CI at 32 to $42^{\circ}C$. While 40-95% operator-binding activity was shown to be retained at 35 to $42^{\circ}C$ in CI, more than 75% operator-binding activity was lost in CIts391 at 35 to $38^{\circ}C$, although the latter showed only 10% less binding compared to that of the former at $32^{\circ}C$. The CIts391 showed almost no binding at $42^{\circ}C$. An in vivo study showed that the CI repressor inhibited the growth of a clear plaque former mutant of the L1 phage more strongly than that of the CIts391 repressor at both 32 and $42^{\circ}C$. The half-life of the CIts391-operator complex was found to be about 8 times less than that of the CI-operator complex at $32^{\circ}C$. Interestingly, the repressor-operator complexes preformed at $0^{\circ}C$ have shown varying degrees of resistance to dissociation at the temperatures which inhibit the formation of these complexes are inhibited. The CI repressor, but not that of CIts391, regains most of the DNA-binding activity on cooling to $32^{\circ}C$ after preincubation at 42 to $52^{\circ}C$. All these data suggest that the 131st proline residue at the C-terminal half of CI, which changed to leucine in the CIts391, plays a crucial role in binding the L1 repressor to the cognate operator DNA, although the helix-turn-helix DNA-binding motif of the L1 repressor is located at its N-terminal end.

LOCAL SPECTRAL PROPERTIES OF SEMI-SHIFTS

  • Yoo, Jong-Kwang;Kim, Yong-Il
    • Journal of applied mathematics & informatics
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    • v.28 no.1_2
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    • pp.499-507
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    • 2010
  • In this note, we study the local spectral properties of semi-shifts. If $T\;{\in}\;L(X)$ is a semi-shift on a complex Banach space X, then T is admissible. We also prove that if $T\;{\in}\;L(X)$ is subadmissible, then $X_T(F)\;=\;E_T(F)$ for all closed $F\;{\subseteq}\;\mathbb{C}$. In particular, every subscalar operator on a Banach space is admissible.

RESULTS ON MEROMORPHIC FUNCTIONS SHARING THREE VALUES WITH THEIR DIFFERENCE OPERATORS

  • LI, XIAO-MIN;YI, HONG-XUN;KANG, CONG-YUN
    • Bulletin of the Korean Mathematical Society
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    • v.52 no.5
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    • pp.1401-1422
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    • 2015
  • Under the restriction of finite order, we prove two uniqueness theorems of nonconstant meromorphic functions sharing three values with their difference operators, which are counterparts of Theorem 2.1 in [6] for a finite-order meromorphic function and its shift operator.

QUASI-INNER FUNCTIONS OF A GENERALIZED BEURLING'S THEOREM

  • Kim, Yun-Su
    • Bulletin of the Korean Mathematical Society
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    • v.46 no.6
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    • pp.1229-1236
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    • 2009
  • We introduce two kinds of quasi-inner functions. Since every rationally invariant subspace for a shift operator S$_K$ on a vector-valued Hardy space H$^2$(${\Omega}$, K) is generated by a quasi-inner function, we also provide relationships of quasi-inner functions by comparing rationally invariant subspaces generated by them. Furthermore, we discuss fundamental properties of quasi-inner functions and quasi-inner divisors.

ELS FTF algorithm fot ARMA spectral estimation (ARMA스펙트럼 추정을 위한 ELS FTF 알고리즘)

  • 이철희;장영수;남현도;양홍석
    • 제어로봇시스템학회:학술대회논문집
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    • 1989.10a
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    • pp.427-430
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    • 1989
  • For on-line ARMA spectral estimation, the fast transversal filter algorithm of extended least squares method(ETS FTF) is presented. The projection operator, a key tool for geometric approach, is used in the derivation of the algorithm. ELS FTF is a fast time update recursion which is based on the fact that the correlation matrix of ARMA model satisfies the shift invariance property in each block, and thus it takes 10N+31 MADPR.

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G/M/1 QUEUES WITH ERLANGIAN VACATIONS

  • Park, Bong-Dae;Han, Dong-Hwan
    • Communications of the Korean Mathematical Society
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    • v.10 no.2
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    • pp.443-460
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    • 1995
  • We consider a G/M/1 vacation model where the vacation time has k-stages generalized Erlang distribution. By using the methods of the shift operator and supplementary variable, we explicitly obtain the limiting probabilities of the queue length at arrival time points and arbitrary time points simultaneously. Operational calculus technique is used for solving non-homogeneous difference equations.

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Novel Approach of a Phage-Based Magnetoelastic Biosensor for the Detection of Salmonella enterica serovar Typhimurium in Soil

  • Park, Mi-Kyung;Chin, Bryan A.
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2051-2059
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    • 2016
  • To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect Salmonella enterica serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cation-exchange resin method, and combinations of both methods were employed for the extraction of S. Typhimurium from soil. The number of S. Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant green sulfa agar plate and an HP 8751A network analyzer. A blocking study was performed using bovine serum albumin (BSA), polyethylene glycol (PEG), and casein powder suspension. Finally, the modified ME biosensor method was performed to detect S. Typhimurium in soil. The number of S. Typhimurium was significantly decreased from 7.10 log CFU/soil to 4.45-4.72 log CFU/soil after introduction of the cation-exchange resin method. The greatest resonant frequency shift of the measurement sensor was found when employing centrifugation and filtration procedures. The resonant frequency shift of the PEG-blocked measurement sensor was $3,219{\pm}755Hz$, which was significantly greater than those of the BSA- and casein-blocked ME sensor. The optimum concentration of PEG was determined to be 1.0 mg/ml after considering the resonant shift and economic issue. Finally, the modified ME biosensor method was able to detect S. Typhimurium in soil in a dose-response manner. Although these modifications of the ME biosensor method sacrificed some advantages, such as cost, time effectiveness, and operator friendliness, this study demonstrated a novel approach of the ME biosensor method to detect S. Typhimurium in soil.

Exposure to Noise on Railroad Operators (철도 차량 운전원의 소음노출 특성)

  • Lee, Sang-Yun;Choi, Sang-Jun
    • Journal of Environmental Health Sciences
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    • v.34 no.2
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    • pp.153-160
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    • 2008
  • Personal noise exposure was assessed in railroad operators working in 40 operation units including the Gyeongbu line, Honam line, and Janghang line between 2004 and 2006. 8-hour TWA were assessed based on the MOL (Ministry of Labor) guideline on noise evaluation, and 17% of these measurements exceeded 85 dBA while 70% were over 80 dBA. When the ACGIH TLV is adopted, 40% of measurements were over TLV (85 dBA). The noise exposure risk of railroad operators was higher for diesel locomotive operation and longer operational distances; in addition, the risk was higher for passenger car operation compared to freight car driving. Given that in Korea, over the past 30 years diesel locomotives have provided most of the railway's motive power and that hearing protective equipment is not likely to be used by workers during operation, railroad operators are at a high risk of noise-induced hearing loss. The result of audiometric test among 568 railroad operators showed that 32.6% of those tested had a hearing threshold shift of more than 40 dB. In conclusion, this study calls for more fundamental measures including noise control countermeasures within the operation areas, development of equipment that generates less noise and adopting limitation on the operation distance for a work shift.

THE DIAGNOSIS OF IMPACTED MAXILLARY MESIODENS USING 3-DIMENSIONAL COMPUTED TOMOGRAPHY : A CASE REPORT (3차원 전산화 단층촬영을 이용한 상악 정중 과잉치의 진단)

  • Hong, Young-Woo;Kim, Seong-Oh;Sohn, Hyung-Kyu;Lee, Jong-Gap
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.3
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    • pp.549-554
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    • 1998
  • The prevalence of the supernumerary tooth is $1{\sim}4%$. It usually occurs in the permanent dentition. It frequently occurs in the maxillary mid palatal area. The presence of the supernumerary tooth causes the following problems ; (1) interfering the eruption of successional tooth, (2) displacing the neighboring teeth, (3) resulting large diastema, (4) forming cystic change. So the supernumerary tooth should be removed as soon as possible. To extract the supernumerary tooth, the exact position must be noticed first. Radiographic techniques that were used in the past are tube shift technique, right angle technique, sterioradiography, using radiopaque contrast media and conventional tomography. But these methods include the subjective opinion of the operator. So, a technique eliminating the operator's opinion and showing the position 3-dimentionally can be used. 3-dimentional computed tomography equipped with dental softwares can show the position of the impacted supernumerally tooth in 3-dimentional position. It has an advantage to eliminate the subjective opinion of the operator. With a fast computer this techenique is done in a relatively short period of time. The rationale of this methods is relatively simple. After tacking X-ray and reconstructing the data 3-dimentionally, sequential removal of the soft tissue and hard tissue(bone) CT number leaves the teeth alone in 3-dimentional position. The image can be seen from anywhere, so the operator can see the image in front, rear, upper, and lower positions. In both cases 1 and 2, the position of the impacted supernumerary tooth is viewed by the 3-dimensional computed tomography. And it made the operator easy to figureout the exact position.

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