• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1988년도 한국자동제어학술회의논문집(국내학술편); 한국전력공사연수원, 서울; 21-22 Oct. 1988
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• pp.482-487
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• 1988

A sequential-clustered integrator based on GEAR method is developed for the purpose of dynamic simulation of chemical processes. And a single simulator structure capable of employing various integration approaches is designed and its efficiency and flexiblity is evaluated. Sequential integration method is superior to simultaneous method for the process without recycle, but simultaneous method is very powerful for the coupled process with recycle.

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.826-830
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• 2018

A Cre/loxP-${\delta}$-integration system was developed to allow sequential and simultaneous integration of a multiple gene expression cassette in Saccharomyces cerevisiae. To allow repeated integrations, the reusable Candida glabrata MARKER (CgMARKER) carrying loxP sequences was used, and the integrated CgMARKER was efficiently removed by inducing Cre recombinase. The XYLP and XYLB genes encoding endoxylanase and ${\beta}$-xylosidase, respectively, were used as model genes for xylan metabolism in this system, and the copy number of these genes was increased to 15.8 and 16.9 copies/cell, respectively, by repeated integration. This integration system is a promising approach for the easy construction of yeast strains with enhanced metabolic pathways through multicopy gene expression.

• 품질경영학회지
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• 제32권3호
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• pp.141-152
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• 2004

This study describes process and method to establish concurrent and/or sequential schedule planning for the system design process of the EIA632 standard. For this purpose, the study suggest Process and method that are context analysis of standard process, interface definition from activity decomposition, integration of related activities, and definition of concurrent and/or sequential work flow. The proposed process and method will contribute to minimize time loss that is emerged from activities iteration.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.453-458
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• 2004

This study proposes a new two-level condensation scheme for the construction of a reduced system. In the first step, the candidate area is selected for the construction of the reduced system by energy estimation in element-level. In the second step, primary degrees of freedom are selected by sequential elimination from the candidate degrees of freedom linked to the selected elements. Numerical examples demonstrate that the proposed method saves the computational cost effectively and provides a reduced system which predicts the eigenvalues accurately. Moreover, the well-constructed reduced system can present the reliable behavior of the structure under arbitrary dynamic loads comparing to that of global system. Time integration in a reduced system can save the computing time remarkably. Through a few numerical examples, the efficiency and reliability of the proposed scheme are verified.

• 생명과학회지
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• 제24권10호
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• pp.1046-1054
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• 2014

${\beta}$-1,3-glucanase는 다양한 바이오공정에 널리 사용되어지는 효소로서 산업적 이용가치 증대를 위해 ${\beta}$-1,3-glucanase의 대량생산이 요구되어 지고 있다. 본 연구에서는 반복서열 ${\delta}$-sequence에 의한 integration를 통해 Aspergillus oryzae유래의 ${\beta}$-1,3-glucanase (EXGA)의 과발현 유도를 연구하였다. 먼저 효모내의 여러 염색체상에 EXGA 유전자를 integration하기 위해 $pRS{\delta}K$-exgA와 $pRS{\delta}K$-exgA 플라스미드를 구축하였다. 이 플라스미드는 유전자의 구성적 발현을 위한 ADH1 프로모터, 분비생산을 위한 signal sequence와 ${\beta}$-1,3-glucanase유전자의 integration을 위한 ${\delta}$-sequence를 포함하고 있다. 먼저 $pRS{\delta}K$-exgA 플라스미드를 $BY4742{\Delta}exg1$ 균주에 형질전환하고, 재조합 ${\beta}$-1.3-glucanase가 안정하게 과발현 및 분비생산됨을 확인하였다. 다음으로 geneticin 선별을 통한 integration 유도와 ${\beta}$-1,3-glucanase 활성과의 관계를 조사해보기 위해 $pRS{\delta}K$-exgA 플라스미드를 $BY4742{\Delta}exg1$ (YKY082) 균주에 도입한 결과, geneticin 농도 증가에 따라 ${\beta}$-1,3-glucanase 활성도 증가되었고, geneticin 농도 0.8 mg/ml가 ${\beta}$-1,3-glucanase과발현에 적합한 농도임을 확인 할 수 있었다. 이어서 $pRS{\delta}K$-exgA 플라스미드는 연속적 ${\delta}$-integration에 의해 효모세포 내에 도입되어, 한번, 두번, 세번 그리고 네번의 integration에 의해 ${\beta}$-1,3-glucanase의 비활성은 0.063, 0.095, 0.131 그리고 0.165 unit/ml/$OD_{600}$로 증가되었다. 또한 연속적 integration에 의해 ${\beta}$-1,3-glucanase의 활성이 증가됨에 따라 다양한 염색체에 도입된 EXGA 유전자의 복제수(integration 빈도)도 같이 증가되었음을 확인하였다. 따라서 본 연구 결과는 반복적 ${\delta}$-sequence integration방법을 통해 재조합 ${\beta}$-1,3-glucanase의 활성을 점진적으로 안정하게 증가시킬 수 있음을 제시하였다.

• 소성∙가공
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• 제21권7호
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• pp.441-446
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• 2012

A sequential coupled field analysis of electromagnetic free bulging was performed by using FEM. A 2D axi-symmetric electromagnetic model based on the magnetic vector potential is proposed for the calculation of magnetic field and Lorentz's forces. The Newmark integration method is used to calculate the transient dynamic plastic deformation of sheet during free bulging. In the finite element model, the effect of sheet deformation on the electromagnetic field analysis is taken into consideration. In order to confirm the sequential electromagnetic-mechanical coupling analysis, an experiment with an electromagnetic forming apparatus was conducted. The results showed that the final bulge height of the sheet predicted from the proposed method is in good agreement with experimentally measured height.

• 한국미생물·생명공학회지
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• 제47권4호
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• pp.667-672
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• 2019

본 연구는 출아효모 Saccharomyces cerevisiae을 이용해 이종 유전자(heterologous gene)를 효모염색체내에 도입하여 안정적으로 발현하기 위한 시스템의 비교에 대해서 연구하였다. 반복적으로 사용할 수 있는 Cre/loxP system의 이용을 위해 C. glabrata 유래 유전자를 선택마커로 사용하였고, universal pRS-CMT vector를 이용한 4종의 유전자(XYLP, XYLB, GRE3 및 XYL2 유전자)를 모델 유전자로 cloning하였다. 구축된 pRS-XylP, pRS-XylB, pRS-Gre3 및 pRS-Xyl2 plasmid를 이용한 4번의 sequential integration을 통해 효모염색체내에 도입된 4종의 유전자를 순차적으로 발현시킬 수 있었다. 또한 4종의 유전자 발현 cassette를 동시에 가지는 pRS-PBG2 plasmid에 의한 one-step integration을 통해서, 도입될 유전자들의 순서를 정할 수 있었으며 각 유전자들의 동시발현을 안정적으로 유지할 수 있었다. 결론적으로 본 연구에서 사용한 4종의 유전자들의 염색체내 동시 integration 및 발현을 위해서는 one-step integration이 효과적임을 확인하였으며, 적절한 유전자 도입방법을 통해 산업적으로 유용한 생물시스템의 손쉬운 육종이 가능하리라 기대한다.

• Asia Marketing Journal
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• 제24권3호
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• pp.96-108
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• 2022

This study investigates how affects elicited by visual images in print ads are integrated to form a liking for the ads. Assuming a sequential rather than simultaneous processing of still-cut images, we adopt the 'think-aloud' method to capture consumers' spontaneous responses to visual images. We hypothesize that not only would consumers show mixed affects toward a still-cut visual image but that they would also integrate their serial affects heuristically rather than simply averaging the affects as suggested by the compensatory hypothesis. By comparing the effects of two contradictory affect integration hypotheses (i.e., peak-affect and mood-maintenance) with compensatory integration, using a single regression model, we found that peak-negative along with mood maintenance integration of serial affects for a print ad works best in the formation of ad liking. The results also support our initial premise that people can have mixed valence even toward a still-cut ad.

• 산업경영시스템학회지
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• 제27권3호
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• pp.31-36
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• 2004

This paper will describe design process and its results that are minimized unaddressed feature of product development processes. This process provides integrated system design process by context analysis of EIA 632 standard process, interface definition from activity decomposition, integration of related activities, and definition of concurrency & sequential activities flow. This process and method application will contribute to minimize time loss that is emerged from activities iteration by not definitely definition of activities interface.

• Journal of Astronomy and Space Sciences
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• 제35권1호
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• pp.39-46
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• 2018

In this study, the performance of ranging techniques for the Korea Pathfinder Lunar Orbiter (KPLO) space communication system is investigated. KPLO is the first lunar mission of Korea, and pseudo-noise (PN) ranging will be used to support the mission along with sequential ranging. We compared the performance of both ranging techniques using the criteria of accuracy, acquisition probability, and measurement time. First, we investigated the end-to-end accuracy error of a ranging technique incorporating all sources of errors such as from ground stations and the spacecraft communication system. This study demonstrates that increasing the clock frequency of the ranging system is not required when the dominant factor of accuracy error is independent of the thermal noise of the ranging technique being used in the system. Based on the understanding of ranging accuracy, the measurement time of PN and sequential ranging are further investigated and compared, while both techniques satisfied the accuracy and acquisition requirements. We demonstrated that PN ranging performed better than sequential ranging in the signal-to-noise ratio (SNR) regime where KPLO will be operating, and we found that the T2B (weighted-voting balanced Tausworthe, voting v = 2) code is the best choice among the PN codes available for the KPLO mission.