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Occurrence of Chrysanthemum chlorotic mottle viroid in Chrysanthemum(Dendranthema grandiflorum) in Korea

  • Chung, Bong-Nam;Kim, Dong-Chan;Kim, Jeong-Soo;Cho, Jeom-Deog
    • The Plant Pathology Journal
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    • v.22 no.4
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    • pp.334-338
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    • 2006
  • Chrysanthemum chlorotic mottle viroid(CChMVd) isolates have been identified from chrysanthemum showing yellow spots or infected without symptom. They were 399-400 nucleotides length of RNA. CChMVd-SSHA6(GenBank accession no. DQ450682) revealed a GAAA to DUUC substitution in positions 82-85 of CChMVd-MSIN34(GenBank accession no. DQ402041). In vitro RNA transcripts with the complete CChMVd sequence were infectious and induced the typical CChMVd infection symptom of yellow spots in chrysanthemum cv. Sharotte. CChMVd caused reduction in growth in some cultivars, whereas some cultivars were not affected. This is the first report on the occurrence of CChMVd in chrysanthemum in Korea.

Acoustic Properties of Yeacdang and its Modifications (국립국악원 예악당에 음향 특성과 개선방향)

  • 박경수
    • Proceedings of the Acoustical Society of Korea Conference
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    • 1998.06e
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    • pp.65-68
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    • 1998
  • 국악의 대중적 공연에 적합한 공연장에 대하여는 아직 그 주관적 객관적 기준이 설립되어 있지 않다. 현재 국립국악원에 있는 예악당은 국악을 위한 대중적 공연장의 기준이 되어야 하나 국악인들과 국악관계자들의 사이에서 국악의 공연에 적합하지 않은 것으로 평가되고 있다. 본 논문에서는 국립국악원의 예악당을, Maximum Length Sequence, 무지향성스피커, dummy head 등을 이용하여 여러 가지 중요한 객관적 지표인 잔향시간, IACC, Initial-time-delay gap등을 측정하고 한 편으로는 컴퓨터 시뮬레이션에 의한 객관적 지표의 추출을 통하여 비교 분석하였다. 그리고, 이를 토대로 예악당의 음향특성을 개선방향과 국악에 대한 주관적 객관적 지표의 설정 방법을 제시하고자 한다.

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Gene Cloning, High-Level Expression, and Characterization of an Alkaline and Thermostable Lipase from Trichosporon coremiiforme V3

  • Wang, Jian-Rong;Li, Yang-Yuan;Liu, Danni
    • Journal of Microbiology and Biotechnology
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    • v.25 no.6
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    • pp.845-855
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    • 2015
  • The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60℃ and kept 45% enzyme activity after 1 h incubation at 70℃. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).

The Implementing a Color, Edge, Optical Flow based on Mixed Algorithm for Shot Boundary Improvement (샷 경계검출 개선을 위한 칼라, 엣지, 옵티컬플로우 기반의 혼합형 알고리즘 구현)

  • Park, Seo Rin;Lim, Yang Mi
    • Journal of Korea Multimedia Society
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    • v.21 no.8
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    • pp.829-836
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    • 2018
  • This study attempts to detect a shot boundary in films(or dramas) based on the length of a sequence. As films or dramas use scene change effects a lot, the issues regarding the effects are more diverse than those used in surveillance cameras, sports videos, medical care and security. Visual techniques used in films are focused on the human sense of aesthetic therefore, it is difficult to solve the errors in shot boundary detection with the method employed in surveillance cameras. In order to define the errors arisen from the scene change effects between the images and resolve those issues, the mixed algorithm based upon color histogram, edge histogram, and optical flow was implemented. The shot boundary data from this study will be used when analysing the configuration of meaningful shots in sequences in the future.

The taxonomic status of Angelica purpuraefolia and its allies in Korea : Inferences based on ITS molecular phylogenetic analyses

  • Lee, Byoung Yoon;Kwak, Myounghai;Han, Jeong Eun;Kim, Se-Jung
    • Korean Journal of Plant Taxonomy
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    • v.41 no.3
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    • pp.209-214
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    • 2011
  • The taxonomy of the umbelliferous species Angelica amurensis and its allies was reviewed on the basis of molecular phylogenies derived from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) regions. Strict consensus of six minimal length 119-step trees derived from equally weighted maximum parsimony analysis of combined nuclear rDNA ITS1 and ITS2 sequences from 29 accessions of Angelica and outgroups indicated that Angelica purpuraefolia, known to be endemic to Korea, is the same species as A. amurensis. Comparisons of sequence pairs across both spacer regions revealed identity or 1-2 bp differences between A. purpuraefolia and A. amurensis. These results indicated that the two taxa are not distinguished taxonomically. Also, nuclear rDNA ITS regions are discussed as potential barcoding loci for identifying Korean Angelica.

Analysis of Melanocortin receptor 1 (MC1R) gene differential test for beef species between Hanwoo and Holstein using polmerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) (MC1R gene의 PCR-RFLP를 이용한 한우.젖소고기 감별)

  • Suh, Dong-Kyun
    • Korean Journal of Veterinary Service
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    • v.31 no.3
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    • pp.369-374
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    • 2008
  • The objective of this study was to differentiate the beef species between Hanwoo and Holstein from a total of 1,081 beef samples using PCR-RFLP of MC1R gene. When a PCR product of 403 bp specific band amplified from bovine MC1R gene sequence was digested with restriction enzyme MspA1I, Hanwoo type showed 2 bands, 220 bp and 183 bp size bands. Holstein type, however, showed three bands, 220 bp, 138 bp and 45 bp size band, respectively. The results of the differential test for beef species were as following; 7 samples (0.64%) were determined to Holstein type, of which 4 were submitted from administrative authorities, other 3 from self-collection planing, and none from civilian clients including school.

Some Analogues of a Result of Vasconcelos

  • DOBBS, DAVID EARL;SHAPIRO, JAY ALLEN
    • Kyungpook Mathematical Journal
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    • v.55 no.4
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    • pp.817-826
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    • 2015
  • Let R be a commutative ring with total quotient ring K. Each monomorphic R-module endomorphism of a cyclic R-module is an isomorphism if and only if R has Krull dimension 0. Each monomorphic R-module endomorphism of R is an isomorphism if and only if R = K. We say that R has property (${\star}$) if for each nonzero element $a{\in}R$, each monomorphic R-module endomorphism of R/Ra is an isomorphism. If R has property (${\star}$), then each nonzero principal prime ideal of R is a maximal ideal, but the converse is false, even for integral domains of Krull dimension 2. An integral domain R has property (${\star}$) if and only if R has no R-sequence of length 2; the "if" assertion fails in general for non-domain rings R. Each treed domain has property (${\star}$), but the converse is false.

A Review on the Sumptuary Laws of Western Costume (서양복식사에 나타난 금제고찰)

  • 김인숙
    • Journal of the Korean Home Economics Association
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    • v.19 no.3
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    • pp.1-9
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    • 1981
  • The object of this study lies in reviewing of the western costume history in the light of the negative regulations concerning costumes and adornments. Readings related with the subject lead to the following findings; 1. the principal purpose of the sumptuary laws has relationship with the social stratification of the feudalism and the frequency of their occurrence is related with the formation of feudal system, its decline, the appearance of imperial regimes and the start of civil revolutions. 2. Sumptuary regulations spread westward with the flow of civilization, eg., in the sequence of Italy, swiss, France, Britain and new world. 3. Sumptuary laws are also found in abundance in the english colonies of America, and their object seems to be rather of the moral concern than class distinction of economical restriction.4. The reviewed sumptuary regulations are concerned with : A. Material gold or silver clothes, silks, ermine, marten or miniver furs, velvets, laces and embroies. B. Colors-Purple, scarlet and red. Other colors do not show consistent pattern of color-status symbol; C. Form-Length and width of headdresses, shoes, collars, trains or hoops. E. numbers of dresses allowed to be worn.

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Frequency Offset Estimation in OFDMA Uplink System Using Low Cross-correlation Property of PN Sequences (OFDMA 상향 링크 시스템에서 PN 시퀀스의 낮은 Cross correlation 특성을 이용한 주파수 오차 추정)

  • Min, Hyun-Kee;Kim, Seok-Joong;Bang, Keuk-Joon;Hong, Dae-Sik
    • Proceedings of the IEEK Conference
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    • 2008.06a
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    • pp.239-240
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    • 2008
  • A new frequency offset estimator using low cross-property of PN sequences is proposed in the orthogonal frequency division multiple access (OFDMA) uplink systems. Simulation results show that the proposed estimator improves the mean square error (MSE) performance compared to conventional estimator [2] where each user have both different timing offset and different frequency offset. In addition, the MSE performance is improved as the length of PN sequence increases.

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Analysis on Dominant Factor for Gait Recognition (걸음걸이 인식을 위한 지배 요소 분석)

  • 박한훈;박종일
    • Proceedings of the IEEK Conference
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    • 2003.11a
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    • pp.321-324
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    • 2003
  • This paper presents a novel system that analyzes and recognizes a gait based on shape context on silhouette images. The main functions of the system consist of three steps: First, the system extracts the silhouette images from galt image sequence by performing a simple pre-processing and acquires the AGM(Averaged Gait Map) by averaging them. Next. it computes the cross-correlation between the AGMs. Finally, it classifies the AGMs based on the cross-correlation using nearest neighborhood classification. The proposed system uses two cues to classify a gait: One corresponds to biometric shape cue such as body height width. and body-part proportions. The other corresponds to gait cue such as stride length and amount of arm swing. Perceptionally, the biometric cues are sailent on the double support (both legs spread and touching the ground) while the gait cues on the midstance. Through a variety of experiments, it is proved that the property of a gait is mainly influenced by gait cues than biometric cues.

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