[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1408.08039

Gene Cloning, High-Level Expression, and Characterization of an Alkaline and Thermostable Lipase from Trichosporon coremiiforme V3

Wang, Jian-Rong (Guangdong VTR Bio-Tech Co., Ltd)
Li, Yang-Yuan (Guangdong VTR Bio-Tech Co., Ltd)
Liu, Danni (Guangdong VTR Bio-Tech Co., Ltd)

Publication Information

Journal of Microbiology and Biotechnology / v.25, no.6, 2015 , pp. 845-855 More about this Journal

Abstract

The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60℃ and kept 45% enzyme activity after 1 h incubation at 70℃. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).

Keywords

Trichosporon coremiiforme; alkaline and thermostable lipase; high-level expression; Pichia pastoris;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)

Reference
Cited By KSCI

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