• Journal of Plant Biotechnology
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• 제43권2호
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• pp.181-188
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• 2016

In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.289-295
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• 2006

국내에서 유통되고 있는 토마토 품종의 판별 방법에 SSR marker의 이용 가능성에 대한 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. 토마토 28품종을 18개의 SSR marker를 이용하여 분석하였을 때 대립유전자의 수는 $2{\sim}9$개로 비교적 다양한 분포를 나타내었으며 전체 60개의 대립유전자가 분석되었다. PIC 값은 $0.476 {\sim}0.800$ 범위에 속하였으며 평균값은 0.607로 나타났다. SSR marker를 이용하여 작성된 토마토 28품종의 품종간 유전적 거리는 $0.35{\sim}0.97$의 범위로 나타났고, 유사도 지수 0.36을 기준으로 할 때 28개 품종은 체리형 토마토 그룹과 일반형 토마토 그룹으로 나눌 수 있었으며, 공시 품종 모두 SSR marker의 genotype에 의해 뚜렷이 구분되었다. 이 연구결과는 토마토의 품종식별에 기초 자료로 유용하게 이용될 수 있는 것으로 나타났다.

• 한국작물학회지
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• 제7권1호
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• pp.133-137
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• 1969

This experiment was conducted to determine the optimum row width and plant spacing within row under the tropical environment for a leading soybean variety Palmetto and it was carried out at Eakmat Experiment Station in Vietnam. The experiments were arranged in a split plot design with four replications and the test was repeated twice in two years. Variations for seed yield due to the distance between rows were significant at 1 per cent level in 1967 test(dry season growing) and at 5 per cent level in 1968 test. Significant differences for plant height, lodging, maturity, number of branches per plant, and number of pods per plant due to the row width were not found in both tests, while significant differences in difference spacing within row was found in all traits studied. Interaction between width of row and spacing within row for seed yield and plant height were found at five percent level in 1967 test. These results indicate that close planting may increase in seed yield and plant height in both seasons, and decrease in loadging. From these studies, one could be understood that the plant population, particularly in dry season, plays decisive roles on seed yield in soybean culture, and the maximum plant height and minimum value of lodging index were also observed in closest spacing plots. The highest soybean yield in late planting would be expected by a combinations of 30 cm(between rows)${\times}$5cm(between hills) plot, while 40cm ${\times}$ 5cm planting method would be suggested for rainy season growing. Highest seed yield was obtained at closet spacing in both dry and rainy season, and these results led to drilling method in seeding where about 20 seeds per meter of row could be recommended. Besides the seed yield, the close planting may produce some advantages, such as increase plant height and decrease lodging, weeds and erosion of surface soil.

• 한국환경복원기술학회지
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• 제2권2호
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• pp.70-77
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• 1999

This study was carried out to suggest an effective method for the rehabilitation of Close-to-Nature River and artificial wetland. The results on the revegetation methods by seeding and sodding of Phragmites japonica, Miscanthus sacchariflorus, Themeda triandra, and Pennisetum alopecuroides were summarized as follows. Seed germination of Phragmites japonica was 76.3% at $30^{\circ}C$, that of Miscanthus sacchariflorus was 68.7% at $20^{\circ}C$, that of Themeda triandra germinated 52.3% at $25^{\circ}C$, $30^{\circ}C$ constant temperature and that of Pennisetum alopecuroides germinated 86.7% at $30^{\circ}C/20^{\circ}C$ alternating temperature. Seed germination of Pennisetum alopecuroides exceeded 80% at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$ constant temperature and $25^{\circ}C/15^{\circ}C$ alternating temperature. At 60 days after seeding, the ground coverage of Phragmites japonica, Miscanthus sacchariflorus, Themeda triandra, and Pennisetum alopecuroides reached 81%, 81 %, 74%, and 86% respectively in the soil media of vermiculite and peatmoss(1 : 1 by volume). In the results of sod experiment, Phragmites japonica, Miscanthus sacchariflorus, Themeda triandra, and Pennisetum alopecuroides were formed sod completely in soil composition type of vermiculite and peatmoss(1 : 1, v/v). Thus this media seems to be best sad production media for rehabilitation works of Close-to-Nature River and man-made wetlands. Phragmites japonica and Miscanthus sacchariflorus were not significantly different in shoot height and the number of tillers by different planting distance for the first one year of experiment. Pennisetum alopecuroides shows high possibility to be used for Close-to-Nature River rehabilitation works by seeding.

• 한국응용곤충학회지
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• 제7권
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• pp.5-13
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• 1969

본 보고에서 서해안 개소의 매개 진딧물 밀도가 대관령에서의 밀도보다 대체로 낮았으나 장소에 따라서는 상당히 높은 사실을 밝힌바 있다. 새 감자의 평지생산방식이 현행방식보다 합리적이어서 유리하므로 그 생산휴계의 확립을 위한 기초자료를 얻고자 본 조사에 착수하였다. 서해안 답작물지대에서의 진딧물 발생원은 부락이므로 여기서 얼마나 떨어진 곳이라야 안전하게 씨감자를 재배할 수 있을 까를 위하여 전북 김제군 광활면 옥포리를 택하여 이곳을 중심으로 1.5km 평방지구내 13개소에 동서남북으로 250m 거리를 두고 진딧물 채집기을 설치하여 1968년 7월 21일부터 10월 31일까지 수집된 진딧물을 매일아침에 채집하여 종명을 밝혔다. 감자바이러스 매개 진딧물이 세계적으로 20종 알려져 있는데 우리나라에 있는 것은 10종이며 이 지역에서 발견된 것은 복숭아 흑진딧물(Myzus persicae), 목화진딧물(Aphis gossypii), 싸리수염진딧물(Aulacorthum solani), 무테두리진딧물(Lipaphis erysimi) 및 국화꼬마수염진딧물 (Macrosiphoniella sanborni)의 5종이었다. 중락중심지에서의 매개진딧물 밀도는 내릉의 수원에서의 그것 보다도 높았으나 여기서 불과 250m만 떨어져도 대관령에서의 밀도 보다 낮아졌다. 그러나 조사지점의 환경에 따라 상당한 차이가 있다. 즉 매개 진딧물의 기주식물이 있는 인가와의 거리에 따라 달라진다. 추작용 씨감자의 현재산지인 전남의 일모, 경남의 락은 내륙의 부락근방이라서 매개진딧물 관계로 부적당하다고 본다. 전문가의 식물역학적 진단에 의해 서해안 답작지대중 매개 진딧물이 적은 곳을 택해서 씨감자를 생산한다면 우수한 씨감자를 양산할 수 있다. 씨감자 부족량 60,000톤을 충족시킨다면 $50\%$ 증수(년간 250만톤)할 수 있어 농가소득증대는 물론 남는 감자는 전분원료로서 단경기에 공급할 수 있으며 일부를 사료화할 수 있고 우수한 씨감자는 외국에 수출할 수 도 있으며 전북 서해안의 광대한 일모작지대를 이모작지대로 만들 수 있다.

• 한국작물학회지
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• 제51권1호
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• pp.26-31
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• 2006

This study was conducted to determine the optimum number of inter-rows according to distance of drainage furrow (DF) for running-off excessive-water stress (EWS) in paddy field. The most soil water potential was shown in high ridge (distance of DF by 70 cm) cultivation and the soil water potential showed increasing tendency in over four inter-rows cultivation by DF. The growth of soybean reduced by extended inter-row and its reducing level was high, especially, over four inter-rows (DF distance by 2.8 m) because of EWS. The photosynthetic rate decreased in the more extensive field by distance of DF at V5 and R2 stages, especially, in over four interrows cultivation. Also, root activity decreased at wider DF. The yield was reduced with wider distance of DF more extensively, the highest yield of 270 g per $m^2$ at the every row, but yield showed decreasing tendency at over the $4^{th}$ row (2.8 m) cultivation. Soybean cultivation in paddy field could be founded with DF of every other or $4^{th}$ row.

• Journal of Biosystems Engineering
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• 제36권6호
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• pp.434-443
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• 2011

A large scale seeding system was stable in terms of techniques but a convenient seeding system of small size was unsettled. This study was performed to develop a semi-automatic seeder for small and medium sized scale farm. To investigate optimum needle diameter and vacuum pressure was used vacuum suction needle seeder. Although the needle diameter according to the kinds of seed was different, the needle diameter for salvia and lettuce seed was suitable for 0.34 mm needle nozzle and 0.4 mm taper nozzle. The prototype consisted a seeding frame attached with needle nozzle, seed hopper, vibrating device, seeding part, vacuum ejector, seed tube etc.. As the result with the experiments, the seeding rate of the seeder was 92% and more at 0.34 mm diameter needle nozzle and 0.4 mm taper nozzle. Eccentric weight for seed hopper vibration was suitable that weight is 11 g and eccentric distance is 0.5 mm. Vibration acceleration of upward direction was 0.363 m/$s^2$. Working capacity of the seeder was possible 160 trays per hour. It was possible for sowing small seeds but it was required to make compact and simple model.

• The Korean Journal of Ecology
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• 제25권1호
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• pp.1-7
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• 2002

The ecological background of a restoration project is complex and difficult to betermine without experimentation. A useful context for experiments is the well-studied process of natural succession, because the factors that drive or inhibit succession are also at work during reclamation (a form of primary succession) and restoration (which often resembles secondary succession). Using experimental studies on urban wasteland reclamation, we have tested for factors that stimulate or inhibit succession during early phases of woodland development in the Northeastern United states. The emphasis has been on mutualisms (seed dispersal, pollination, and mycorrhizae) and microsite limitations in the recruitment, growth, and reproduction of woody plants. Using plantings of seeds, seedlings, and clusters of reproductively mature plants on abandoned landfills, we have observed that (1) soil microsite deficiencies lead to very poor germination (<0.1$\%$) and seedling survival (<0.01$\%$) of most native species; (2) seed dispersal by birds is a significant and reliable source of woody plant recruitment; however (3) proximity effects are strong, with most (up to 95$\%$) of seed rain falling in the vicinity of planted clusters that are closest to putative seed sources; and (4) remnant natural woodlands are critical components of the recruitment process. To emphasize the last point, in one case, we found that the destruction of approximately 50$\%$ of nearby natural woodland vegetation led to a commensurate decline in seed rain. In another case, we found that the species richness of recruits was strictly limited by the species composition of nearby source plant communities, with no evidence of community enrichment by long distance dispersal over 5 years. We conclude from these results that the size and proximity of remnant natural populations are critical considerations when planning reclamation and restoration programs that rely on natural successional processes.

• 한국농림기상학회지
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• 제15권4호
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• pp.234-244
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• 2013

본 연구는 느티나무 집단의 종자특성 변이에 영향을 미치는 요인과 종자특성이 유묘생장에 미치는 영향을 구명하고자 수행되었다. 이를 위해 느티나무16개 집단에서 종자를 채취하여 특성을 조사하였으며, 포지에서 1년간 양묘한 후 묘목의 생장을 조사했다. 종자특성과 유묘생장에 영향을 미치는 요인을 구명하고자 조사형질과 종자채취 집단의 지리적기후적 인자와의 상관분석을 실시했다. 종자특성에 대한 분산분석 결과, 모든 측정형질에서 집단 간에 유의한 차이가 있었다. 지리적 요인과의 상관을 분석한 결과, 종자길이와 종자폭은 위도, 경도, 해발고, 해안으로부터 거리와 정의 상관을 보여준 반면, 종자형상비는 부의 상관을 나타냈다. 기후인자와의 상관을 분석한 결과, 종자길이와 종자폭은 종자채취 집단의 연평균 기온 및 생육기(3~8월)의 평균기온과 부의 상관을 나타냈으며, 종자형상비는 이들과 정의 상관을 보여주었다. 천립중은 지리적요인과 상관이 없었고, 기후인자 중 건조지수와 유의하지만 낮은 정의 상관을 나타냈다. 용적중은 위도, 경도와 낮은 부의 상관을 나타냈으며, 생육기 (3~8월)의 평균 습도와 정의 상관을 나타냈다. 종자충실율은 위도, 경도와 유의한 부의 상관을 보였으며, 건조지수와는 유의한 부의 상관을 나타냈다. 유묘의 생장은 묘고와 근원경 모두 집단 간, 집단 내 가계 간 그리고 가계 내 개체 간에 유의한 차이가 있는 것으로 나타났다. 묘고와 근원경은 모두 경도 및 생육기(3~8월) 평균 습도와 유의한 부의 상관을 나타냈다. 종자특성과 유묘생장의 상관을 분석한 결과, 묘고생장은 종자특성과 상관이 없었으며, 근원경은 천립중 및 용적중과 유의하지만 낮은 부의 상관을 나타냈다. 이러한 점을 볼 때, 느티나무 1년생 유묘의 생장은 종자특성의 영향이 크지 않은 것으로 판단되었다. 전반적으로 볼때, 종자특성과 유묘생장은 집단 간에 유의한 차이가 있었지만, 지리적 또는 기후적 요인과의 상관성이 낮아 그 경향성이 뚜렷하지 않은 것으로 나타났다. 따라서, 1년생 유묘에 대한 분석만으로는 느티나무 집단의 유전적 특성 및 지리적 변이를 정확히 추정하기 어렵다고 판단되며, 추후 산지시험을 통한 조림지 적응성 및 생장특성 검정 결과를 토대로 느티나무 집단에 대한 평가가 이루어져야 할 것으로 보인다.

• 한국식물병리학회지
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• 제14권5호
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• pp.484-490
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• 1998

Microorganisms associated with seed-rhizome rot of gingers preserved in three underground storage caves were identified with respect to rot types. Rot patterns were grouped into 4 different types : yellow soft rot, brown rot, localized ring rot, and water-soaked rot. Water-soaked rot was highest in frequency with 40% and ring rot the least with 14%. Causal pathogens differed with rot type, yellow soft rot by Erwinia carotovora and Pseudomonas aeruginosa, brown rot by Fusarium solani and Pseudomonas aeruginosa, localized ring rot by F. solani, and water-soaked rot by Pythium spinosum and P. ultimum. Pythium myriotylum, the causal pathogen of ginger rhizome rot which occurs severely in fields was rarely detected from storage seed-rhizomes suggesting its minor involvement with storage rot. Pathogenic Pythium isolates were frequently obtained from both rhizome surface and inner tissues of rotten rhizomes. Detection frequency of Pythium isolates in inner tissues decreased as increasing distance from rhizome surface. In wound-inoculation tests, above pathogens caused a varying degree of rot on healthy rhizomes at 15$^{\circ}C$, 2$0^{\circ}C$ and 3$0^{\circ}C$ with increasing severity at higher temperatures.