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http://dx.doi.org/10.5010/JPB.2006.33.4.289

Use of Simple Sequence Repeat (SSR) Markers for Variety Identification of Tomato (Lycopersicon esculentum)  

Kwon, Yong-Sham (Variety Testing Division, National Seed Management Office, MAF)
Park, Eun-Kyung (Variety Testing Division, National Seed Management Office, MAF)
Bae, Kyung-Mi (Variety Testing Division, National Seed Management Office, MAF)
Yi, Seung-In (Variety Testing Division, National Seed Management Office, MAF)
Park, Soon-Gi (Variety Testing Division, National Seed Management Office, MAF)
Cho, Il-Ho (Variety Testing Division, National Seed Management Office, MAF)
Publication Information
Journal of Plant Biotechnology / v.33, no.4, 2006 , pp. 289-295 More about this Journal
Abstract
This study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for varietal identification and genetic diversity in 28 commercial tomato varieties. The relationship between marker genotypes and 28 varieties was analyzed. Of the 219 pairs of SSR primers screened against ten tomato varieties, 18 pairs were highly polymorphic with polymorphism information content (PIC) ranging from 0.467 to 0.800. Among the polymorphic loci, two to nine SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Genetic distances were estimated according to Jaccard's methods based on the probability that the amplified fragment from one genotype would be present in another genotype. These varieties were categorized into cherry and classic fruit groups corresponding to varietal types and genetic distance of cluster ranging from 0.35 to 0.97. The phonogram discriminated all varieties by marker genotypes. The SSR markers proved to be useful variety identification and genetic resource analysis of tomato.
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