Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Use of Simple Sequence Repeat (SSR) Markers for Variety Identification of Tomato (Lycopersicon esculentum)

Simple Sequence Repeat (SSR) Marker를 이용한 토마토 품종 식별

  • Kwon, Yong-Sham (Variety Testing Division, National Seed Management Office, MAF) ;
  • Park, Eun-Kyung (Variety Testing Division, National Seed Management Office, MAF) ;
  • Bae, Kyung-Mi (Variety Testing Division, National Seed Management Office, MAF) ;
  • Yi, Seung-In (Variety Testing Division, National Seed Management Office, MAF) ;
  • Park, Soon-Gi (Variety Testing Division, National Seed Management Office, MAF) ;
  • Cho, Il-Ho (Variety Testing Division, National Seed Management Office, MAF)
  • 권용삼 (농림부 국립종자관리소 재배시험과) ;
  • 박은경 (농림부 국립종자관리소 재배시험과) ;
  • 배경미 (농림부 국립종자관리소 재배시험과) ;
  • 이승인 (농림부 국립종자관리소 재배시험과) ;
  • 박순기 (농림부 국립종자관리소 재배시험과) ;
  • 조일호 (농림부 국립종자관리소 재배시험과)
  • Published : 2006.12.30
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Abstract

This study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for varietal identification and genetic diversity in 28 commercial tomato varieties. The relationship between marker genotypes and 28 varieties was analyzed. Of the 219 pairs of SSR primers screened against ten tomato varieties, 18 pairs were highly polymorphic with polymorphism information content (PIC) ranging from 0.467 to 0.800. Among the polymorphic loci, two to nine SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Genetic distances were estimated according to Jaccard's methods based on the probability that the amplified fragment from one genotype would be present in another genotype. These varieties were categorized into cherry and classic fruit groups corresponding to varietal types and genetic distance of cluster ranging from 0.35 to 0.97. The phonogram discriminated all varieties by marker genotypes. The SSR markers proved to be useful variety identification and genetic resource analysis of tomato.

국내에서 유통되고 있는 토마토 품종의 판별 방법에 SSR marker의 이용 가능성에 대한 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. 토마토 28품종을 18개의 SSR marker를 이용하여 분석하였을 때 대립유전자의 수는 $2{\sim}9$개로 비교적 다양한 분포를 나타내었으며 전체 60개의 대립유전자가 분석되었다. PIC 값은 $0.476 {\sim}0.800$ 범위에 속하였으며 평균값은 0.607로 나타났다. SSR marker를 이용하여 작성된 토마토 28품종의 품종간 유전적 거리는 $0.35{\sim}0.97$의 범위로 나타났고, 유사도 지수 0.36을 기준으로 할 때 28개 품종은 체리형 토마토 그룹과 일반형 토마토 그룹으로 나눌 수 있었으며, 공시 품종 모두 SSR marker의 genotype에 의해 뚜렷이 구분되었다. 이 연구결과는 토마토의 품종식별에 기초 자료로 유용하게 이용될 수 있는 것으로 나타났다.

Keywords

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  1. Assessment of genetic variation among commercial tomato (Solanum lycopersicum L) varieties using SSR markers and morphological characteristics vol.31, pp.1, 2009, https://doi.org/10.1007/BF03191132