• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.9B
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• pp.822-831
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• 2008

This paper examines the theoretical and empirical technology diffusion processes to understand the Korean success in the internet access service markets. In order to do this, first, we propose an integrated demand diffusion model in terms of competition of inter-and intra-technologies and market structure as represented by the number of operators in the market. Second, by using the proposed model, we analyze the dynamic diffusion processes of Korean internet access services such as Narrow-band technology including Dial modem vs. Broad-band technology including ISDN, xDSL and Cable modem. The competition of inter-and intra-technologies as well as the extent of market competition has made a positive effect on the diffusion patterns of internet access demand. And also we propose, based on the proposed model and its empirical results, several implications for diffusion strategies and policies in the future of ICT market in Korea.

• Journal of Digital Convergence
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• v.14 no.4
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• pp.487-494
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• 2016

The purpose of this study was to examine the effect of Health exercise program on insulin resistant and blood lipid factor of elderly women for 12 weeks. The two groups were classified into one group(exercise group: EG) with aerobics exercise and band exercise both, the other group(control group: CG) controled. The group of EG was applied to doing aerobics exercise and band exercise 4 times for 60 minutes a week. The intensity of the exercise was ACSM. Each measurement variable was measured before and after 12 weeks to investigate the effect. During this study the result came out with this step. First, EG have shown interaction with blood lipid(TC, HDL, LDL, TG) and blood pressure(SBP, DBP)factor. Second, EG have shown interaction with insulin resistant(insulin, glucose). Therefore, this study gives us positive result to effect of health exercise program on blood lipid factor and insulin resistant factor of elderly women for 12 weeks.

• Journal of the Korea Convergence Society
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• v.9 no.12
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• pp.393-400
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• 2018

This study was to verify the effects of education in expression activity and various convergence education found in the class scene of one elementary school man teather to research of qualitative pattern and analyzes how it is expanded. This study were made through two class participation observation and six video analysis. In addition, we measured in-depth interviews of participants and students, a 10-week jump band training program, and applied a step-by-step physical activity program to enhance creative expressiveness. Based on the results of the study, the conclusions are as follows. First, It was found that the Jump-band training positively affects the students' speed, agility, and cardiopulmonary endurance. Second, It was found that the application of the step-by-step physical expression activity program had a positive effect on the students themselves. Third, It was found that it has an educational effect on the whole life of students beyond the curriculum and the field by attempting various fusion education. Based on this study, we propose the educational effects of the pre - curricular activities and the convergence lessons in elementary school during the follow - up research.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.422-427
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• 2003

Streptomyces setonii (ATCC 39116) is a Gram-positive thermophilic soil actinomycetes capable of degrading single aromatic compounds including phenol and benzoate via ortho-cleavage pathway. we isolated approximately 6.3-kb S. setonii DNA fragment containing a thermophilic catechol 1,2-dioxygenase(C12O) gene. Here we further revealed that the 6.3-kb S. setonii DNA fragment was organized into two putative divergently-transcribed clusters with 6 complete and one incomplete open reading frames (ORFs). The first cluster with 3 ORFs showed significant homologies to previously known benA, benB, and benC, implying a part of benzoate catabolic operon. The second cluster revealed an ortho-cleavage catechol catabolic operon with three translationally-coupled ORFs (catR, catB, catA). Each of these individually-cloned ORFs was expressed in E. coli and identified as a distinct protein band with a theoretical molecular weight in SDS-PAGE. The expression of the cloned S. setonii catechol operon was induced in a heterologous S. lividans by specific single aromatic compounds including catechol, phenol, and 4-chlorophenol. The simitar induction pattern was also observed using a luciferase gene-fused reporter system, implying that S. setonii employs an inducer-specific regulatory mechanism for aromatic compound metabolism.

• Parasites, Hosts and Diseases
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• v.37 no.3
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• pp.157-162
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• 1999

Serum from mouse orally ingested with tissue cyst forming stain (Me49) of Toxoplasma gondii was assayed by Western blot and immunofluorescene assay (IFA) to establish early responses in antigenicity of the parasite in mouse model of foodborne toxoplasmosis. Sera were collected weekly to blot the RH antigen transferred onto nitrocellulose paper after being separated by 12% SDS-PAGE. With the second week serum, 34 kDa protein (p34) was detected uniquely, and all antigens of T.gondii were detected with the sera from 3 or 4 weeks. p34 was not a member of the major surface membrane proteins and confirmed to be localized in the rhoptry by IFA. It was secreted into parasitophorous vacuolar membrane (PVM) during the entry into host cells. 10.3% of sera detected p34, while all the ELISA positive sera detected the band. It has diagnostic usefulness of presumed T.gondii infection. We suggest the name of the p34 protein as ROP9.

• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.40-48
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• 2003

Cylindrocarpon destructans is the major pathogen inducing the root rot disease in ginseng. Up to now, there is no reliable and convenient method to analyze the spore density or population of this pathogen in ginseng-growing soil or any contaminated farmlands. Therefore, it will be very valuable to develop a new and reliable method in detecting the spore of this pathogen. In this study, a molecular biological technique using two step nested PCR method, was developed. Two universal ITS primers, ITS5F and ITS4R were used in the first round of PCR to amplify a fragment of ITS region from the genomic DNA of C. destructans. The specific prmers Nest 1 and Nest 2 were designed and used in the second round of PCR to amplify a inner fragment from the first round PCR product of C. destructans. C. destructans spore, only soil samples from the diseased ginseng farm produced the positive bands, suggesting its usefulness in detecting the C. destructans spores in soil samples. Thus it is recommended to first extract the whole genomic DNA from soil samples and use it for the PCR reaction, thereby eliminating the inhibitory activity of soil components.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.5989-5994
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• 2012

Background: There is little information on longitudinal patterns of participation in faecal occult blood test (FOBT) based colorectal cancer (CRC) screening or on demographic or behavioural factors associated with participation in re-screening. The lack of an agreed system for describing participatory behaviour over multiple rounds also hampers our ability to report, understand and make use of observed associations. Our aims were to develop a system for describing patterns of participatory behaviour in FOBT-based CRC screening programs and to identify factors associated with particular behavioural patterns. Methods: A descriptive framework was developed and applied to a data extract of screening invitation outcomes over two rounds of the NBCSP. The proportion of invitees in each behaviour category was determined and associations between behaviour patterns and demographic and program factors were identified using multivariate analyses. Results: We considered Re-Participants, Dropouts, Late Entrants and Never Participants to be the most appropriate labels for the four possible observed participatory categories after two invitation rounds. The screening participation rate of the South Australian cohort of the NBCSP remained stable over two rounds at 51%, with second round Dropouts (10.3%) being balanced by Late Entrants (10.5%). Non-Participants comprised 38.7% of invitees. Relative to Re-Participants, Dropouts were older, more likely to be female, of lower SES, had changed their place of residence between offers had a positive test result in the first round. Late Entrants tended to be in the youngest age band. Conclusions: Specific demographic characteristics are associated with behavioural sub-groups defined by responses to 2 offers of CRC screening. Targeted group-specific strategies could reduce dropout behaviour or encourage those who declined the first invitation to participate in the second round. It will be important to keep first round participants engaged in order to maximise the benefit of a CRC screening program.

• The Journal of Korean Medicine
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• v.28 no.2 s.70
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• pp.80-92
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• 2007

Objectives : Functional dyspepsia (FD) is one of the most common gastrointestinal diseases. Nevertheless, there are many unknown mechanisms of autonomic functioning in FD patients. This study was designed to investigate the relationship between FD and biosignals from heart rate variability (HRV) and Yangdorak diagnosis. Methods : 32 patients (22 female, 10 male; mean age 40) and 32 healthy volunteers (21 female, 11 malemean; age 38) participated in this study. First gastrointestinal symptoms rating scale (GSRS) was assessed by questionnaires in both groups to evaluate the types of gastrointestinal symptoms. Second, HRV and Yangdorak diagnosis were measured in both groups. Results : 1. The FD group in this study mainly had the complaint of 'bloating' symptoms. 2. There was statistically no significant difference between Yangdorak (total average and 24 acupoints) and HRV values except logarithmic low-frequency band (lnLF) and total power (TP) in frequency domain. 3. There was statistically no significant relationship between HRV and Yangdorak in either group. However, most Yangdorak values were positively related with some HRV values (low-frequency, low-frequency/high-frequency ratio and high-frequency, etc) in the control group. Conclusions : FD patients had relatively lesser sympathetic domain than healthy subjects, indicated by decreased lnLF and TP. Particularly, there were positive relationships and significant differences between Yangdorak and HRV in young healthy subjects. This suggests that biosignals from HRV may be a useful method that can differentiate FD from healthy state in those of young age.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Science of Emotion and Sensibility
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• v.23 no.2
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• pp.3-12
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• 2020

The purpose of this work is to suggest the optimal color temperature, which induces a sense of comfort for autonomous vehicle users through the analysis of biosignal using electroencephalography (EEG) and photoplethysmography (PPG). To achieve this purpose, we applied lighting with a color temperature of 3000 K, 4000 K, 5000 K, and 6000 K to the autonomous driving environment. We experimented in a laboratory equipped with a graphic driving simulator. The experimental procedure is as follows: 1) stabilization (5 min). 2) Uchida-Kraepelin test (3 min). 3) Automatic driving + lighting (3 min). This procedure was repeated four times under different color temperatures. We performed frequency analysis on a collected time-series data and calculated the power value for each frequency band through power spectrum analysis. In the case of EEG, we analyzed α- and β-waves, which are indicators of stability and arousal, respectively. In the case of PPG, we analyzed the sympathetic nervous system activity. To reduce deviations between the subjects, we normalized the data before analysis. The result of the first analysis revealed that α-wave increased only at 5000 K, while the β-wave increased at almost all color temperatures. In addition, in the case of PPG, sympathetic nervous system activity (SNSA) increased under driving conditions. The result of the second analysis revealed that the difference between β-wave and SNSA is insignificant. In conclusion, the increase in α-waves showed that EEG was most stable at 5000 K. The results of this study can be applied to the upcoming autonomous driving era to induce high driver satisfaction. Furthermore, this approach could eventually lead to the acceptance of autonomous vehicles by suggesting a positive effect of autonomous driving.