• 한국축산식품학회지
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• 제35권5호
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• pp.707-713
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• 2015

Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.

• Journal of Korean Biological Nursing Science
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• 제13권3호
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• pp.211-219
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• 2011

Purpose: The study compared the rest-activity rhythm and sleep pattern of elderly with young group. Methods: The subjects were 22 over than 65 years old and 23 under 65 years old. An actigraph, sleep diary, Pittsburgh Sleep Quality Index and Insomnia Severity Index scale were used as measurement tools for this study. The data were analyzed with $x^2$, Lamda test, t-test and correlation with SPSS 15.0 program. Results: The elderly had lower curve than the young group in rest-activity rhythm on each time zone. In particular, the elderly group had lower rest-activity rhythm curve of 8, 9, 14, 18, 19, 20, 21, 22 and 23 time zone than those of young group. Sleep pattern had statistical difference in the total sleep time, PSQI and insomnia. Total sleep time of elderly had lower score and PSQI and Insomnia had higher score than young group. Age had correlation with rest-activity rhythm, sleep efficiency, PSQI and insomnia. Conclusion: Rest-activity rhythm of the elderly showed an increase in activity in the early morning because of earlier get up than the young group and an decrease in activity in the afternoon because of taking a nap at this time. Elderly sleep was that total sleep time increased but sleep efficiency decreased and insomnia intensified. This sleep pattern was related to age and rest-activity rhythm.

• BMB Reports
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• 제51권1호
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• pp.5-13
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• 2018

Formation of toxic protein aggregates is a common feature and mainly contributes to the pathogenesis of neurodegenerative diseases (NDDs), which include amyotrophic lateral sclerosis (ALS), Alzheimer's, Parkinson's, Huntington's, and prion diseases. The transglutaminase 2 (TG2) gene encodes a multifunctional enzyme, displaying four types of activity, such as transamidation, GTPase, protein disulfide isomerase, and protein kinase activities. Many studies demonstrated that the calcium-dependent transamidation activity of TG2 affects the formation of insoluble and toxic amyloid aggregates that mainly consisted of NDD-related proteins. So far, many important and NDD-related substrates of TG2 have been identified, including $amlyoid-{\beta}$, tau, ${\alpha}-synuclein$, mutant huntingtin, and ALS-linked trans-activation response (TAR) DNA-binding protein 43. Recently, the formation of toxic inclusions mediated by several TG2 substrates were efficiently inhibited by TG2 inhibitors. Therefore, the development of highly specific TG2 inhibitors would be an important tool in alleviating the progression of TG2-related brain disorders. In this review, the authors discuss recent advances in TG2 biochemistry, several mechanisms of molecular regulation and pleotropic signaling functions, and the presumed role of TG2 in the progression of many NDDs.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1456-1463
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• 2009

Vibrio parahaemolyticus possessing urease-positive property is relatively rare, but such strains consistently exhibit the TDH-related hemolysin (TRH) gene. In this study, we examined the effects of incubation temperature on urease activity expression, using the TH3996 and AQ4673 strains where the enzyme activity is known to be temperature-dependent and -independent, respectively. In the TH3996 strain, $\beta$-galactosidase activity was 4.4-fold lower after $30^{\circ}C$ cultivation than after $37^{\circ}C$ in a ureR-lacZ fusion strain, but temperature dependency was not found in ureD- or nikA-lacZ fusion strains. However, ureR-, ureD-, and nikA-lacZ fusions of the AQ4673 strain was not influenced by incubation temperature. We compared the promoter sequences of ureR between the above two strains. Intriguingly, we detected mismatches of two nucleotides between the two strains located at positions -66 and -108 upstream of the methionine initiation codon for UreR. Additionally, urease activity was not affected by culture temperature at either $30^{\circ}C$ or $37^{\circ}C$ by allelic introduction of the AQ4673 ureR gene into the TH3996 ureR deletion mutant. Taken together, our study demonstrates that the transcriptional factor UreR is involved in the temperature dependency of urease activity, and two nucleotides within the ureR promoter region are of particular importance for the urease activity dependency of V. parahaemolyticus.

• 지역사회간호학회지
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• 제19권3호
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• pp.506-520
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• 2008

Purpose: The purpose of this study was to investigate factors influencing college students' health promotion lifestyle. Methods: The subject was 606 students. Statistical analysis with SPSS used descriptive statistics, t-test, one way ANOVA, Pearson correlation and Stepwise Multiple Regression. Results: The average item score for health promotion lifestyle was 2.58. The subscale showing the highest score was interpersonal relationship (3.03), which was followed by spiritual growth (2.94). stress management (2.54), nutrition (2.52), physical activity (2.16) and health responsibility (2.15). There were significant differences according to age, gender, BMI, perceived health state, religion, economic state, live together, major and health promotion lifestyle. The most powerful Predictors of health promotion lifestyle were the prior related behavior (51.8%) and self-efficacy (7.7%). The combination of prior related behavior, perceived self-efficacy, activity-related affect, social support, perceived stress, commitment to a plan of action accounted for 67.9% of the variance of health promotion lifestyle. Conclusion: Prior related behavior was the most powerful variable of health promotion lifestyle. Therefore, health promotion programs for changing and maintaining prior related behavior and increasing self-efficacy should be developed to promote a healthy lifestyle in college students.

• 인지과학
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• 제16권4호
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• pp.255-270
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• 2005

항목이 연달아 반복되는 경우(집중반복)보다 간격을 두고 반복되는 경우(간격반복) 기억 수행이 더 우수한 현상을 간격효과라 한다. 단서기억과제(재인과제)의 간격효과 및 성공적 부호화와 관련된 신경기전을 밝히기 위해 event-related fMRI연구를 수행하였는데, 부호화단계에서 단어의 반복제시 간격을 조작하고서 fMRI 영상을 구하였다. 그 결과, 재인 수행에서 전형적인 간격효과가 관찰되었고 집중반복조건에 비해 간격반복조건 뿐만 아니라 비반복조건에서 좌반구의 배외측 전두피질(DLFC), 복외측 전두피질(VLFC)의 높은 활성화가 관찰되었다. 이러한 결과는 간격효과 및 이와 관련된 성공적 부호화가 높은 수준의 주의통제와 의미적 처리에 기인한다는 것을 시사한다.

• 한국지역사회생활과학회지
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• 제25권3호
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• pp.373-382
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• 2014

This study was explored the relationship of fit-related factors and, clothing evaluation criteria to clothing satisfaction. A total of 247 completed responses were obtained from a convenient sample of female students in their twenties. The statistical analysis methods included factor analysis, an ANOVA, and a multiple regression analysis. First, clothing evaluation criteria included practicality, product trait, and symbolism. Second, a comparison of clothing evaluation criteria and clothing satisfaction in groups divided by fit-related factors showed significant differences. In particular, the fat body type group showed higher level of the product trait and the thin body type group showed lower level of clothing satisfaction. The high activity group showed higher levels of clothing evaluation criteria and lower level of clothing satisfaction. Third, body type perception (-) and activity importance (-), fit-related factors, and practicality (+), clothing evaluation criterion had significant effects on clothing satisfaction. Therefore, These results suggest that fashion firms should make efficient use of fit-related factors and clothing evaluation criteria to enhance consumers' clothing satisfaction and to stimulate their purchase intention toward fashion goods.

• 대한간호학회지
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• 제35권1호
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• pp.104-112
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• 2005

Purpose: This research was conducted to determine the effects of a fall prevention program on knowledge, self-efficacy, prevention activity, and depression in the low-income elderly women. Method: The design of this study was a nonequivalent control group pretest-posttest design. There were 22 subjects in the experimental group and 22 in the control group. A fall prevention program was performed for approximately 40 minutes, once a week for 6 weeks. Results: The results of this study were as follows: There were statistically significant differences of knowledge, self-efficacy, and prevention activity related to falls and depression between the experimental group and control group. Conclusion: In conclusion, the fall prevention program used in this study is appropriate for the elderly, therefore this program is strongly recommended for community-based health and welfare centers.

• Journal of Applied Biological Chemistry
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• 제58권1호
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• pp.5-8
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• 2015

This study was to isolate an active component of the chloroform fraction from the methanol extract of Ruta chalepensis leaves and to measure inhibitory effects against ${\alpha}$-glucosidase or ${\alpha}$-amylase. The inhibitory compound of R. chalepensis leaves was isolated using chromatographic methods and identified as quinoline. Quinoline and its structurally related derivatives were tested for their inhibitory activities by evaluating the $IC_{50}$ values against ${\alpha}$-amylase or ${\alpha}$-glucosidase and were compared with that of acarbose. Based on the $IC_{50}$ values, quinazoline exhibited the greatest inhibitory activity ($20.5{\mu}g/mL$), followed by acarbose ($66.5{\mu}g/mL$), and quinoline ($80.3{\mu}g/mL$) against ${\alpha}$-glucosidase. In case of ${\alpha}$-amylase, quinazoline had potent inhibitory activity, followed by quinoline ($179.5{\mu}g/mL$) and acarbose ($180.6{\mu}g/mL$). These results indicate that R. chalepensis extract, quinoline, and quinazoline could be useful for inhibiting ${\alpha}$-glucosidase or ${\alpha}$-amylase.

• 대한지구과학교육학회지
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• 제3권2호
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• pp.124-131
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• 2010

The purpose of this study is examine relationship of attribution styles and attitude toward Science and Science Process Skills of Science-gifted, to understand unique characteristics of the Science-gifted and to give useful information that can be use in develop special programs for the Science-gifted. The result of this study were as follows: First, there was no difference between genders. But there was a significant difference in attribution of luck. Second, there was a correlation between internal tendencies and Scientific attitude. Especially attribution of effort correlated with sub - constituent of Scientific attitude. Internal tendencies correlated with the Cognition in Scientific Professions that sub - constituent of the attitude toward Science. Third, There was a correlation between external tendencies and the interest activity in Science that sub - constituent of the attitude toward Science. There are correlations between sub - constituent of the attitude toward Science and sub - constituent of external tendencies that attribution of luck and interest in Scientific Professions, attribution of task difficulty and Cognition activity in Science. Fourth There was no correlation between Attribution styles and Science Process Skills. But Internal tendencies correlated with classification that sub - constituent of Science Process Skills. And classification correlated with attribution of ability that sub - constituent of external tendencies. Attribution of effort that sub - constituent of internal tendencies correlated with Science Process Skills.