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http://dx.doi.org/10.5851/kosfa.2015.35.5.707

Whitening Effect of Watersoluble Royal Jelly from South Korea  

Han, Sang Mi (Department of Agricultural Biology, National Academy of Agricultural Science)
Kim, Jung Min (Department of Agricultural Biology, National Academy of Agricultural Science)
Hong, In Phyo (Department of Agricultural Biology, National Academy of Agricultural Science)
Woo, Soon Ok (Department of Agricultural Biology, National Academy of Agricultural Science)
Kim, Se Gun (Department of Agricultural Biology, National Academy of Agricultural Science)
Jang, Hye Ri (Department of Agricultural Biology, National Academy of Agricultural Science)
Park, Kwan Kyu (Catholic University of Daegu School of Medicine)
Pak, Sok Cheon (School of Biomedical Sciences, Charles Sturt University)
Publication Information
Food Science of Animal Resources / v.35, no.5, 2015 , pp. 707-713 More about this Journal
Abstract
Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.
Keywords
royal jelly; water-soluble royal jelly; α -MSH; tyrosinase; TRP-1; TRP-2;
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Times Cited By KSCI : 4  (Citation Analysis)
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