• 생약학회지
• /
• 제45권2호
• /
• pp.147-153
• /
• 2014

This study was focused on the evaluation of radical scavenging effect and the protective activity against oxidative stress of the extract and fractions from Petasites japonicus. P. japonicus was extracted with methanol and then fractionated into 4 fractions [n-butanol, ethyl acetate (EtOAc), methylene chloride, and n-hexane]. The extract and fractions showed strong 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Among all the fractions, particularly, the EtOAc fraction showed the strongest effect with the $IC_{50}$ value of $0.02{\mu}g/ml$. In addition, the fractions also showed strong hydroxyl radical scavenging activity and nitric oxide scavenging activity as well. Furthermore, cell viability generated by the P. japonicus extract and 4 fractions were examined under C6 glial cellular model. The C6 glial cells showed high generation of reactive oxygen species (ROS) and decrease in cell viability by the treatment generator of hydrogen peroxide. However, the production of ROS formation was decreased by the treatment of the fractions of P. japonicus and also founded that the EtOAc fraction led to significant increase in the cell viability at concentration $100{\mu}g/ml$. Results from this work indicated that P. japonicus showed protective effects against oxidative stress and its EtOAc fraction may be served as a useful natural antioxidant.

• 생약학회지
• /
• 제43권4호
• /
• pp.297-301
• /
• 2012

In the course of screening for antioxidant compounds by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), a total extract of the twigs of Taxillus delavayi (Loranthaceae) was found to show potent radical scavenging activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of two phenolic compounds, catechin (1) and galloyl catechin (2), as active principles. Their structures were elucidated by spectroscopic studies. These two compounds showed the significant antioxidative effects on DPPH, and riboflavin- and xanthin-originated superoxide quenching activities. And these two compounds also showed strong nitric oxide radical scavenging effects. In riboflavin- and xanthin-originated superoxide quenching activities and nitric oxide radical scavenging effect, compound 2 showed better antioxidant activities than vitamin C.

• 한국식품조리과학회지
• /
• 제16권4호
• /
• pp.372-377
• /
• 2000

Applemint, geranium, lemonbalm, rosemary, sage, thyme등 6종 herb의 기능성을 검토하기 위하여 물 및 95% methanol로 추출하여 얻은 추출액의 전자공여능, 항균작용 및 아질산염 소거능을 조사하였다. 전자공여능은 60.7∼92.2%로 95% methanol추출물이 물추출물보다 높게 나타났고 geranium, sage, lemonbalm, thyme, rosemary, applemint 순으로 작용이 우수하였다. 각종 herb추출물의 항균력은 물보다 methanol추출물이 우수하였고 gram음성균보다는 양성균주에서 효과적이었으며 geranium과 rosemary 공시균주에 대하여 광범위한 항균활성을 나타내었고 분획별 항균력 측정결과 geranium은 극성용매 분획에서, rosemary는 비극성 용매 분획에 항균력이 집중되어 있음을 확인하였다. 아질산염 소거능 역시 물추출물 보다는 methanol추출물에서 높은 활성을 보였고 pH 1.2에서 가장 효과적이었으며 pH가 증가할수록 감소하였다.

• Archives of Pharmacal Research
• /
• 제28권7호
• /
• pp.804-809
• /
• 2005

The EtOAc-soluble fraction from a methanolic extract of Hovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1-8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds 6 and 8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound 6 had a DPPH free radical scavenging effect with an $IC_{50}$ value of $57.7{\mu}M$, and a superoxide anion radical scavenging effect with an $IC_{50}$ value of $8.0{\mu}M$. Both compounds 6 and 8 had ABTS cation radical scavenging effects with $IC_{50}$ values of $7.8{\mu}M\;and\;23.7${\mu}M$, respectively. These results suggest that compounds 6 and 8 could be neuroprotectants owing to their free radical scavenging activities.

• Journal of Applied Biological Chemistry
• /
• 제62권2호
• /
• pp.167-172
• /
• 2019

Napier grass (Penninsetum purpureum)의 50% 에탄올 추출물의 수율, 폴리페놀 함량 및 항산화 활성을 확인하였다. Napier grass 추출물의 수율은 $6.3{\pm}0.35%$이었고, 총 페놀성 화합물 함량은 $79.6{\pm}3.65{\mu}g$ gallic acid equivalents/mg-추출물로 나타났다. Napier grass 추출물의 유리라디칼과 양이온라디칼 소거능, 환원력, 아질산염 소거능 및 지질과산화 억제능은 모두 추출물의 농도에 비례하여 증가하였다. Napier grass 추출물의 DPPH 유리라디칼에 대한 $EC_{50}$값은 $1,930.0{\mu}g/mL$로 유리라디칼에 대한 소거활성이 높지 않았다. Napier grass 추출물의 ABTS 양이온라디칼 소거, 환원력 및 아질산염 소거에 대한 $EC_{50}$값은 각각 350.0, 840.0 및 $1,470.0{\mu}g/mL$로 나타나 유리라디칼 소거활성에 비해 높은 항산화 능력을 보유하고 있었다. 특히 Napier grass 추출물은 $85.0{\mu}g/mL$의 농도에서 과산화물의 생성을 74.6% 억제할 정도로 뛰어난 지질과산화 억제활성을 보여주었다.

• 한국식품저장유통학회지
• /
• 제22권5호
• /
• pp.708-713
• /
• 2015

고구마 잎을 건조 채소화 하기 위한 기초자료를 제공하기 위해서 열풍건조한 고구마 잎 메탄올 추출물에 함유된 총 탄닌, 총 플라보노이드, 총 폴리페놀 함량을 분석하고, 항산화 효과로 DPPH 라디칼 소거활성, ABTS 라디칼 소거능, 아질산염 소거능 등을 분석 비교 검토하였다. 총 탄닌 함량은 신미 $40^{\circ}C$에서 10.87 mg/g, $70^{\circ}C$에서 7.28 mg/g으로 감소되었고, 총 플라보노이드는 하얀미 $40^{\circ}C$에서 55.37 mg/g, $70^{\circ}C$에서 39.63 mg/g으로 감소되었다. 즉 저온건조가 고온건조보다 이들 물질의 함량이 많았다. DPPH 라디칼소거 활성은 신미와 하얀미 $40^{\circ}C$에서 84.33%와 85.25%로 가장 높았으며, ABTS 라디칼 소거능도 $40^{\circ}C$ 처리구에서 80%가 넘는 높은 값이었다. 아질산염 소거능은 신미와 하얀미가 $40^{\circ}C$에서 76.15%와 73.74%로 가장 높았고 $70^{\circ}C$에서는 낮았다. 품종 간에는 하얀미가 신미보다 페놀성 물질과 항산화 물질이 더 많은 것으로 나타났다. 즉 건조한 고구마 잎의 항산화 효과는 품종간의 차이는 있었으나, $40^{\circ}C$ 시료에서 높고, $70^{\circ}C$ 시료에서 낮았다. 이는 건조온도에 의해 영향을 받는 것으로서 낮은 온도에서 건조하는 것이 유효성분의 감소가 적어 항산화 능력이 높은 결과이었다.

• 생약학회지
• /
• 제44권2호
• /
• pp.193-199
• /
• 2013

We investigated the antioxidative and antimicrobial activities of the methanol extracts from Korean folk plants (MKs) in Chungcheong Province. Among 30 MKs, 16 plants at $100{\mu}g/ml$ showed over 90% scavenging activity of 1,1-diphenyl-2-picrylhydrazy (DPPH) and 30 plants exerted the hydroxyl radical scavenging effect over 55%. Fourteen plants at the concentration $50{\mu}g/30{\mu}l$ showed strong microbial inhibition activity against Escherichia coli and Staphylococcus aureus, with clear zone greater than 11 mm in disc assays. Furthermore, the protective effect against anti-inflammatory system using RAW 264.7 macrophage cell was also studied. The treatment of LPS & INF-${\gamma}$ to RAW 264.7 cell induced nitric oxide (NO), however inhibit the formation of NO less than 50% of 5 plants. The present result indicates that the 30 species of MKs exerts protective effect of oxidative stress, antimicrobial activities and anti-inflammatory. In particular, Rhus javanica and Cornus controversa showed stronger effect on not only radical scavenging activity and inhibits growth of S. aureus but also highest protective effects from inflammation.

• 한국식품영양학회지
• /
• 제15권2호
• /
• pp.97-103
• /
• 2002

감귤류(자몽. 레몬, 금귤, 밀감 및 오렌지)를 착즙하여 얻은 쥬스를 rep-pak $C_{18}$ cartridge를 사용하여 as-corbate 및 phenolic 획분으로 분리한 다음 pH를 달리한 in vitro 반응계에서 아질산염 소거 및 N-nitroso-dimenthylamine(NDMA) 생성 억제 효과를 분석하였다. 감귤류 쥬스ascorbate 획분의 아질산염 소거능은 5ml 첨가시 pH 2.5에서 79.9~98.6%, pH 4.2에서 48.5~86.3%였으나, pH 6.0에서는 35.2% 이하였다. Phenolic 획분은 ascorbate 획분에 비해 높은 소거능을 보였으며 특히 pH 6.0에서는 ascorbate 획분에 비해 2배 이상의 높은 소거능을 나타내었다. NDMA 생성억제 효과는 phenolic 획분의 첨가시에 월등히 뛰어나 PH 2.5의 반 응용액에 금귤, 밀감 및 오렌지쥬스의 phenolic 획분을 첨가했을 때 92.8% 이상이었으나, ascorbate 획분이 첨가된 경우에는 NDMA 생성억제 효과를 거의 나타내지 못하였다. 따라서 감귤류 쥬스의 NDMA생성억제와 관련된 주된 인자는 pheno 화합물인 것으로 사료된다.

• 한방안이비인후피부과학회지
• /
• 제34권4호
• /
• pp.24-36
• /
• 2021

Objectives : This study was conducted to confirm the anti-oxidative effect of Chungpyesagan-tang(CPSGT) extract. Methods : In this study, MTT assay was performed to confirm cell viability, and DPPH and ABTS were performed to confirm radical scavenging ability. The ROS scavenging ability and the protective effect against DNA damage were confirmed by 2,7-dichlorofluorescin diacetate(DCF-DA) and 4',6-diamidino-2-phenylindole(DAPI) staining and comet assay. mRNA expression of Heme oxygenase-1(HO-1) was measured by real-time PCR, and expression of HO-1 and Kelch-like ECH-associated protein 1(Keap1) proteins was measured by western blot. Results : CPSGT was not cytotoxic at 50-400㎍/㎖. The radical scavenging activity was increased, and the ROS scavenging activity and the protective effect against DNA damage were increased compared to the LPS-treated group. The mRNA expression and protein expression of HO-1 were increased in a concentration-dependent manner. The protein expression level of Keap1 was decreased in a concentration-dependent manner. Conclusion : This suggests that CPSGT has an antioxidant effect and can be used as a potential material for skin diseases.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권11호
• /
• pp.1553-1561
• /
• 2004

Two feeding trials were conducted with 128 pullets from 4 to 20 weeks of age and 96 laying hens from 23 to 63 weeks of age to evaluate the effects of scavenging and type of protein supplement on the feed intake and performance of improved pullets and laying hens. The experiments had a completely randomized design with four dietary treatments and four replicates. Treatments were: Control (Cont), scavenging but with access to a balanced concentrate at night; confinement (CF) and given the control feed ad libitum; scavenging and supplemented at night with the control feed, but with soybean meal replaced by cassava leaf meal (CLM); scavenging and supplemented at night with the control feed, but with fishmeal replaced y soybean meal (SBM). The mean daily dry matter (DMI), metabolizable energy (MEI) and crude protein intakes (CPI) of the pullets and laying hens, respectively, were 28%, and 18% higher for the confinement treatment (CF) compared to the scavenging treatments (p<0.001). The DMI, MEI and CPI of the pullets were not significantly different among scavenging treatments (p>0.05), but for the layers DMI, MEI and CPI were significantly higher for the CLM and SBM treatments compared to the Cont treatment (p<0.001). In the growing period, the average daily weight gain (ADG), supplement feed conversion ratio (FCR) and supplement feed cost/kg eggs (FCS) were not significantly different for CF compared to Cont, and among scavenging treatments (p>0.05). In the laying period, the hen-day production was significantly lower, and supplement FCR and FCS significantly higher for the CF compared to the scavenging treatments (p<0.001). Egg weight, and yolk, albumen and shell percentage and shape index were not significantly different among the scavenging treatments (p>0.05). However, shell and yolk percentages were significantly lower for the CF compared to the Cont treatment (p<0.01). Mortality was significantly higher for the CF compared to the scavenging treatments for pullets, and was significantly lower for the CF compared to scavenging treatments for laying hens (p<0.001). It was concluded that scavenging pullets and layers were getting around 28% and 18%, respectively, of their nutrient requirements from scavenging activities, resulting in correspondingly lower supplement feed conversion ratios and feed costs. Daily gains of the pullets were not affected by scavenging or protein supplement, but egg production and mortality were lower for the confined hens.