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Neuroprotective and Free Radical Scavenging Activities of Phenolic Compounds from Hovenia dulcis  

Li, Gao (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology, College of Pharmacy, Yanbian University)
Min, Byung-Sun (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology)
Zheng, Chang-Ji (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology, College of Pharmacy, Yanbian University)
Lee, Joong-Ku (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology)
Oh, Sei-Ryang (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology)
Ahn, Kyung-Seop (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology)
Lee, Hyeong-Kyu (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Archives of Pharmacal Research / v.28, no.7, 2005 , pp. 804-809 More about this Journal
Abstract
The EtOAc-soluble fraction from a methanolic extract of Hovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1-8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds 6 and 8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound 6 had a DPPH free radical scavenging effect with an $IC_{50}$ value of $57.7{\mu}M$, and a superoxide anion radical scavenging effect with an $IC_{50}$ value of $8.0{\mu}M$. Both compounds 6 and 8 had ABTS cation radical scavenging effects with $IC_{50}$ values of $7.8{\mu}M\;and\;23.7${\mu}M$, respectively. These results suggest that compounds 6 and 8 could be neuroprotectants owing to their free radical scavenging activities.
Keywords
Hovenia dulcis; HT22 Cells; Neuroprotection; (-)-Catechin; (+)-Afzelechin; DPPH; ABTS; Superoxide;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 9  (Related Records In Web of Science)
Times Cited By SCOPUS : 7
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