Browse > Article
http://dx.doi.org/10.6114/jkood.2021.34.4.024

Anti-oxidative Effect of Chungpyesagan-tang in LPS Induced RAW 264.7 Cells  

Jeon, Bo-Hee (Dept Of Cosmetic Sciences Semyung University)
Kim, Tae-Jun (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Kim, Hee-Taek (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Kim, Yong-Min (Dept. Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.34, no.4, 2021 , pp. 24-36 More about this Journal
Abstract
Objectives : This study was conducted to confirm the anti-oxidative effect of Chungpyesagan-tang(CPSGT) extract. Methods : In this study, MTT assay was performed to confirm cell viability, and DPPH and ABTS were performed to confirm radical scavenging ability. The ROS scavenging ability and the protective effect against DNA damage were confirmed by 2,7-dichlorofluorescin diacetate(DCF-DA) and 4',6-diamidino-2-phenylindole(DAPI) staining and comet assay. mRNA expression of Heme oxygenase-1(HO-1) was measured by real-time PCR, and expression of HO-1 and Kelch-like ECH-associated protein 1(Keap1) proteins was measured by western blot. Results : CPSGT was not cytotoxic at 50-400㎍/㎖. The radical scavenging activity was increased, and the ROS scavenging activity and the protective effect against DNA damage were increased compared to the LPS-treated group. The mRNA expression and protein expression of HO-1 were increased in a concentration-dependent manner. The protein expression level of Keap1 was decreased in a concentration-dependent manner. Conclusion : This suggests that CPSGT has an antioxidant effect and can be used as a potential material for skin diseases.
Keywords
Chungpyesagan-tang(CPSGT); Anti-oxidative; ROS; Heme-oxygenase 1; DNA damage;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Shin DH. Research trends and directions of natural antioxidants. Food Science and Industry. 1997;30(1):14-21.
2 Cho SH, Choi YJ, Rho CW, Choi CY, Kim DS, Cho SH. Reactive Oxygen Species and Cytotoxicity of Bamboo. J Food Preserv. 2008;15(1):105-10.
3 Won JS. Dongeuisasangshinpyeon. Seoul:Jonghapeuiwonsa.1974:66-7.
4 Faculties of Korean medical pathology in the Korean Medical Colleges. Korean Medical Pathology. 2nd ed. Seoul:Omdbook. 2009:58-308.
5 Loft S, Astrup A, Buemann B, Poulsen HE. Oxidative DNA damage correlates with oxygen consumption in humans. FASEB J. 1994;8(8):534-7.   DOI
6 Lopes S, Jurisicova A, Sun JG. Casper RF. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum Reprod. 1998;13(4):896-900.   DOI
7 Maines MD. The heme oxygenase system: a regulator of second messenger gases. Annu Rev Pharmacol Toxicol. 1997;37:517-54.   DOI
8 Kong JH, Lee KY, Rho DH, Lee JC, An WG, Lee BK. Antioxidant and Anti-nflammatory Activites of Tetragonia tetragonoides Water Extract. Herbal Formula Science. 2020;28(2):137-45.   DOI
9 Choi DJ, Jung WS, Moon SK, Cho KH, Kim YS, Bae HS. The Clinical Efficacy of Chungpyesagan-tang on Acute Storke. J Korean Orental Med. 2002;23(4):9-14.
10 Zhu C, Dong Y, Liu H, Ren H, Cui Z. Hesperetin protects against H2O2-triggered oxidative damage via upregulation of the Keap1-Nrf2/HO-1 signal pathway in ARPE-19 cells. Biomed Pharmacother. 2017;88:124-33.   DOI
11 Moloney JN., Cotter TG. ROS signalling in the biology of cancer. Semin Cell Dev Biol. 2018;80:50-64.   DOI
12 Yun HJ, Lee JH, Lee SW, Kim YS, Lee SG, Lee KS. One case of Middle Cerebral Artery Infarction Patient Prescribed Chungpyesagan-tang. Korean J. Orent. Int. Med. 2007;28(1):199-207.
13 Jung GS, Kim BH, Hwang WD. Effect of Chungpaesagan-tang on Cerebral Ischemic Damage Induced by MCAO in Rats. Kor. J. Oriental Preventive Medical Society. 2009;13(1):13-27.
14 Hong SK, Kang BJ, Kim YJ, Kang SM, Cho DW. Protective effects of Chungpesagan-tang against ischemia/reperfusion induced cell injury. Korean J. Orent. Int. Med. 1999;5(1):111-7.
15 Park SN. Skin aging and antioxidants. J Korean Soc Cosmetic Chem. 1997;23(1):75-132.
16 Mittler R. ROS Are Good. Trends Plant Sc. 2017;22(1):11-9.   DOI
17 Veskoukis AS. Tsatsakis AM, Kouretas D. Dietary oxidative stress and antioxidant defense with an emphasis on plant extract administration. Cell Stress Chaperones. 2012;17(1):11-21.   DOI
18 Hong JG. A Study on Skin Aging Caused by Free-Radical and on Efficacy of Antioxidant Vitamins. Kor J Aesthet Cosmetol. 2009;7(2):51-62.
19 Jung HS, Noh KH, Cho HY, Park JY, Choi CY, Kwon TW, et al. Effect of buchu (Allium tuberosum) on lipid peroxidation and antioxidative defense system instreptozotocin-induced diabetic rats. J. Life Sci. 2003;13(3):333-42.   DOI
20 Indo HP, Davidson M, Yen HC, Suenaga S, Tomita K, Nishii T, et al. Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage. 2007;7(1-2):106-18.   DOI
21 Koo JS, Kim JH. Effects of Chungpyesagantang on the diabetic rats induced by streptozotocin. J Korean Orient Pediatr. 1997;11(1):227-48.
22 Srinivas, US, Tan BWQ, Vellayappan BA, Jeyasekharan AD. ROS and the DNA damage response in cancer. Redox Biol. 2019;25:101084.   DOI
23 Jeon SH, Yu JS, Lee EJ. Clinical Practice Guideline for Taeeumin Disease of Sasang Constitutional Medicine: Liver Heat-based Interior Heat (Gansuyeol-liyeol) disease. J Sasang Constitut Med. 2015;27(1):57-70.   DOI
24 Park YJ, Kim SJ, Yang GE, Lee MJ, Lee JS, Kang DH, et al. Anti-platelet Aggregation Effect of Cheongpyesagan-tang In Vitro. Korean J. Orent. Int. Med. 2010;31(4):714-21.
25 Kim EY, Sul YK, Choi JJ, Jeon SH, Kim H, Kim JW. Effect of Cheongpesagan-tang on the change of inhibitory effect against lipase activity and weight loss, plasma and UCP1, 2mRNA expression in db/db mouse. J Sasang Const Med. 2007;19(1):171-85.
26 Kim, TY, Leem KH. Effect of Cheongpyesagan-tang on LPS Induced Inflammation in RAW 264.7 Cells, J of Physiology & Pathology in Korean Med. 2019;33(1):31-8.   DOI
27 Kim JH, Park SS. The Effect of Chungpyesagantang on Lipopolysaccharide induced Arthritis in Mice. J Sasang Const Med. 2002;14(3):114-31.
28 Jung HY, Aging Free radical Arteriosclerosis. Life Science. 1991;1(1):2-14.
29 Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-26.   DOI
30 Soh YJ. Protective Effect of Metabolized Chunpesagan-tang on Hypoxia/Reperfusion Induced-PC12 Cell Damage. Kor J. Pharmacogn. 2005;36(2):151-7.
31 Lee TS, Chau LY. Heme oxygenase-1 mediates the anti-inflammatory effect of interleukin-10 in mice. Nat Med. 2002;8(3):240-6.   DOI
32 de Souza LF, Barreto F, da Silva EG, Andrades ME, Guimaraes ELM, Behr GA, Moreira JCF, et al. Regulation of LPS stimulated ROS production in peritoneal macrophages from alloxan-induced diabetic rats: Involvement of high glucose and PPAR γ. Life Sciences. 2007;81(2):153-9.   DOI
33 Rowe LA, Degtyareva N, Doetsch PW. DNA damage-induced reactive oxygen species (ROS) stress response in Saccharomyces cerevisiae. Free Radic Biol Med. 2008;45(8):1167-77.   DOI
34 Zhao CR, Gao ZH, Qu XJ. Nrf2-ARE signaling pathway and natural products for cancer chemoprevention. Cancer Epidemiol. 2010;34(5):523-33.   DOI
35 National Sasang Medicine University Textbook Editing Board. Sasang Medicine. 2nd ed. Seoul:Jipmoondang. 2011:280-300.
36 Otterbein LE, Choi AMK. heme-oxyganase : colors of defense against cellular stress. Am J Physiol Lung Cell Mol Physiol. 2000;279(6):1029-37.   DOI
37 Balogun E, Hoque M, Gong P, Killeen E, Green CJ, Foresti R, et al. Curcumin activates the heme oxygenase-1 gene via regulation of Nrf2 and the antioxidant responsive element. Biochem. J. 2003;371(3):887-95.   DOI
38 Lee JM. Dongeuisusebowon. Seoul:Henglim Publishing Inc. 1986:7-138.
39 Lee JH, Lee EJ. Clinical Practice Guideline for Taeeumin and Taeyangin Disease of Sasang Constitutional Medicine: Diagnosis and Algorithm. J Sasang Constitut Med. 2015;27(1):13-41.   DOI