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Antimicrobial Activities and Free Radical Scavenging Effect of Korean Folk Plants  

Choi, Jung Ran (Department of Food Science and Nutrition, Pusan National University)
Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University)
Ku, Jajung (Department of Forest Resource Conservation, Korea National Arboretum)
Lee, Sang Yong (Department of Forest Resource Conservation, Korea National Arboretum)
Kim, Hyun Ji (Department of Forest Resource Conservation, Korea National Arboretum)
Park, Kwang-Woo (Department of Forest Resource Conservation, Korea National Arboretum)
Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
Publication Information
Korean Journal of Pharmacognosy / v.44, no.2, 2013 , pp. 193-199 More about this Journal
Abstract
We investigated the antioxidative and antimicrobial activities of the methanol extracts from Korean folk plants (MKs) in Chungcheong Province. Among 30 MKs, 16 plants at $100{\mu}g/ml$ showed over 90% scavenging activity of 1,1-diphenyl-2-picrylhydrazy (DPPH) and 30 plants exerted the hydroxyl radical scavenging effect over 55%. Fourteen plants at the concentration $50{\mu}g/30{\mu}l$ showed strong microbial inhibition activity against Escherichia coli and Staphylococcus aureus, with clear zone greater than 11 mm in disc assays. Furthermore, the protective effect against anti-inflammatory system using RAW 264.7 macrophage cell was also studied. The treatment of LPS & INF-${\gamma}$ to RAW 264.7 cell induced nitric oxide (NO), however inhibit the formation of NO less than 50% of 5 plants. The present result indicates that the 30 species of MKs exerts protective effect of oxidative stress, antimicrobial activities and anti-inflammatory. In particular, Rhus javanica and Cornus controversa showed stronger effect on not only radical scavenging activity and inhibits growth of S. aureus but also highest protective effects from inflammation.
Keywords
Antimicrobial activity; DPPH; Hydroxyl radical; NO; RAW 264.7 macrophage cell;
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