The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-oxidative Effect of Chungpyesagan-tang in LPS Induced RAW 264.7 Cells

LPS로 유도된 RAW 264.7 세포에서 청폐사간탕(淸肺瀉肝湯)의 항산화 효과

  • Jeon, Bo-Hee (Dept Of Cosmetic Sciences Semyung University) ;
  • Kim, Tae-Jun (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Hee-Taek (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Yong-Min (Dept. Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
  • 전보희 (세명대학교 화장품과학과) ;
  • 김태준 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김용민 (세명대학교 화장품뷰티생명공학부)
  • Received : 2021.10.01
  • Accepted : 2021.11.11
  • Published : 2021.11.25
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was conducted to confirm the anti-oxidative effect of Chungpyesagan-tang(CPSGT) extract. Methods : In this study, MTT assay was performed to confirm cell viability, and DPPH and ABTS were performed to confirm radical scavenging ability. The ROS scavenging ability and the protective effect against DNA damage were confirmed by 2,7-dichlorofluorescin diacetate(DCF-DA) and 4',6-diamidino-2-phenylindole(DAPI) staining and comet assay. mRNA expression of Heme oxygenase-1(HO-1) was measured by real-time PCR, and expression of HO-1 and Kelch-like ECH-associated protein 1(Keap1) proteins was measured by western blot. Results : CPSGT was not cytotoxic at 50-400㎍/㎖. The radical scavenging activity was increased, and the ROS scavenging activity and the protective effect against DNA damage were increased compared to the LPS-treated group. The mRNA expression and protein expression of HO-1 were increased in a concentration-dependent manner. The protein expression level of Keap1 was decreased in a concentration-dependent manner. Conclusion : This suggests that CPSGT has an antioxidant effect and can be used as a potential material for skin diseases.

Keywords

Acknowledgement

본 연구는 2021년도 세명대학교 대학혁신 지원 사업에 의해 진행되었습니다.

References

  1. Park SN. Skin aging and antioxidants. J Korean Soc Cosmetic Chem. 1997;23(1):75-132.
  2. Mittler R. ROS Are Good. Trends Plant Sc. 2017;22(1):11-9. https://doi.org/10.1016/j.tplants.2016.08.002
  3. Moloney JN., Cotter TG. ROS signalling in the biology of cancer. Semin Cell Dev Biol. 2018;80:50-64. https://doi.org/10.1016/j.semcdb.2017.05.023
  4. Veskoukis AS. Tsatsakis AM, Kouretas D. Dietary oxidative stress and antioxidant defense with an emphasis on plant extract administration. Cell Stress Chaperones. 2012;17(1):11-21. https://doi.org/10.1007/s12192-011-0293-3
  5. Hong JG. A Study on Skin Aging Caused by Free-Radical and on Efficacy of Antioxidant Vitamins. Kor J Aesthet Cosmetol. 2009;7(2):51-62.
  6. Jung HS, Noh KH, Cho HY, Park JY, Choi CY, Kwon TW, et al. Effect of buchu (Allium tuberosum) on lipid peroxidation and antioxidative defense system instreptozotocin-induced diabetic rats. J. Life Sci. 2003;13(3):333-42. https://doi.org/10.5352/JLS.2003.13.3.333
  7. Srinivas, US, Tan BWQ, Vellayappan BA, Jeyasekharan AD. ROS and the DNA damage response in cancer. Redox Biol. 2019;25:101084. https://doi.org/10.1016/j.redox.2018.101084
  8. Indo HP, Davidson M, Yen HC, Suenaga S, Tomita K, Nishii T, et al. Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage. 2007;7(1-2):106-18. https://doi.org/10.1016/j.mito.2006.11.026
  9. Otterbein LE, Choi AMK. heme-oxyganase : colors of defense against cellular stress. Am J Physiol Lung Cell Mol Physiol. 2000;279(6):1029-37. https://doi.org/10.1152/ajplung.2000.279.6.L1029
  10. Balogun E, Hoque M, Gong P, Killeen E, Green CJ, Foresti R, et al. Curcumin activates the heme oxygenase-1 gene via regulation of Nrf2 and the antioxidant responsive element. Biochem. J. 2003;371(3):887-95. https://doi.org/10.1042/BJ20021619
  11. Lee JM. Dongeuisusebowon. Seoul:Henglim Publishing Inc. 1986:7-138.
  12. National Sasang Medicine University Textbook Editing Board. Sasang Medicine. 2nd ed. Seoul:Jipmoondang. 2011:280-300.
  13. Lee JH, Lee EJ. Clinical Practice Guideline for Taeeumin and Taeyangin Disease of Sasang Constitutional Medicine: Diagnosis and Algorithm. J Sasang Constitut Med. 2015;27(1):13-41. https://doi.org/10.7730/JSCM.2015.27.1.013
  14. Jeon SH, Yu JS, Lee EJ. Clinical Practice Guideline for Taeeumin Disease of Sasang Constitutional Medicine: Liver Heat-based Interior Heat (Gansuyeol-liyeol) disease. J Sasang Constitut Med. 2015;27(1):57-70. https://doi.org/10.7730/JSCM.2015.27.1.057
  15. Kong JH, Lee KY, Rho DH, Lee JC, An WG, Lee BK. Antioxidant and Anti-nflammatory Activites of Tetragonia tetragonoides Water Extract. Herbal Formula Science. 2020;28(2):137-45. https://doi.org/10.14374/HFS.2020.28.2.137
  16. Choi DJ, Jung WS, Moon SK, Cho KH, Kim YS, Bae HS. The Clinical Efficacy of Chungpyesagan-tang on Acute Storke. J Korean Orental Med. 2002;23(4):9-14.
  17. Yun HJ, Lee JH, Lee SW, Kim YS, Lee SG, Lee KS. One case of Middle Cerebral Artery Infarction Patient Prescribed Chungpyesagan-tang. Korean J. Orent. Int. Med. 2007;28(1):199-207.
  18. Jung GS, Kim BH, Hwang WD. Effect of Chungpaesagan-tang on Cerebral Ischemic Damage Induced by MCAO in Rats. Kor. J. Oriental Preventive Medical Society. 2009;13(1):13-27.
  19. Hong SK, Kang BJ, Kim YJ, Kang SM, Cho DW. Protective effects of Chungpesagan-tang against ischemia/reperfusion induced cell injury. Korean J. Orent. Int. Med. 1999;5(1):111-7.
  20. Soh YJ. Protective Effect of Metabolized Chunpesagan-tang on Hypoxia/Reperfusion Induced-PC12 Cell Damage. Kor J. Pharmacogn. 2005;36(2):151-7.
  21. Park YJ, Kim SJ, Yang GE, Lee MJ, Lee JS, Kang DH, et al. Anti-platelet Aggregation Effect of Cheongpyesagan-tang In Vitro. Korean J. Orent. Int. Med. 2010;31(4):714-21.
  22. Kim, TY, Leem KH. Effect of Cheongpyesagan-tang on LPS Induced Inflammation in RAW 264.7 Cells, J of Physiology & Pathology in Korean Med. 2019;33(1):31-8. https://doi.org/10.15188/kjopp.2019.02.33.1.31
  23. Koo JS, Kim JH. Effects of Chungpyesagantang on the diabetic rats induced by streptozotocin. J Korean Orient Pediatr. 1997;11(1):227-48.
  24. Kim JH, Park SS. The Effect of Chungpyesagantang on Lipopolysaccharide induced Arthritis in Mice. J Sasang Const Med. 2002;14(3):114-31.
  25. Kim EY, Sul YK, Choi JJ, Jeon SH, Kim H, Kim JW. Effect of Cheongpesagan-tang on the change of inhibitory effect against lipase activity and weight loss, plasma and UCP1, 2mRNA expression in db/db mouse. J Sasang Const Med. 2007;19(1):171-85.
  26. Jung HY, Aging Free radical Arteriosclerosis. Life Science. 1991;1(1):2-14.
  27. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-26. https://doi.org/10.4103/0973-7847.70902
  28. Shin DH. Research trends and directions of natural antioxidants. Food Science and Industry. 1997;30(1):14-21.
  29. Cho SH, Choi YJ, Rho CW, Choi CY, Kim DS, Cho SH. Reactive Oxygen Species and Cytotoxicity of Bamboo. J Food Preserv. 2008;15(1):105-10.
  30. Won JS. Dongeuisasangshinpyeon. Seoul:Jonghapeuiwonsa.1974:66-7.
  31. Faculties of Korean medical pathology in the Korean Medical Colleges. Korean Medical Pathology. 2nd ed. Seoul:Omdbook. 2009:58-308.
  32. Loft S, Astrup A, Buemann B, Poulsen HE. Oxidative DNA damage correlates with oxygen consumption in humans. FASEB J. 1994;8(8):534-7. https://doi.org/10.1096/fasebj.8.8.8181672
  33. de Souza LF, Barreto F, da Silva EG, Andrades ME, Guimaraes ELM, Behr GA, Moreira JCF, et al. Regulation of LPS stimulated ROS production in peritoneal macrophages from alloxan-induced diabetic rats: Involvement of high glucose and PPAR γ. Life Sciences. 2007;81(2):153-9. https://doi.org/10.1016/j.lfs.2007.04.035
  34. Rowe LA, Degtyareva N, Doetsch PW. DNA damage-induced reactive oxygen species (ROS) stress response in Saccharomyces cerevisiae. Free Radic Biol Med. 2008;45(8):1167-77. https://doi.org/10.1016/j.freeradbiomed.2008.07.018
  35. Lopes S, Jurisicova A, Sun JG. Casper RF. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum Reprod. 1998;13(4):896-900. https://doi.org/10.1093/humrep/13.4.896
  36. Zhao CR, Gao ZH, Qu XJ. Nrf2-ARE signaling pathway and natural products for cancer chemoprevention. Cancer Epidemiol. 2010;34(5):523-33. https://doi.org/10.1016/j.canep.2010.06.012
  37. Lee TS, Chau LY. Heme oxygenase-1 mediates the anti-inflammatory effect of interleukin-10 in mice. Nat Med. 2002;8(3):240-6. https://doi.org/10.1038/nm0302-240
  38. Maines MD. The heme oxygenase system: a regulator of second messenger gases. Annu Rev Pharmacol Toxicol. 1997;37:517-54. https://doi.org/10.1146/annurev.pharmtox.37.1.517
  39. Zhu C, Dong Y, Liu H, Ren H, Cui Z. Hesperetin protects against H2O2-triggered oxidative damage via upregulation of the Keap1-Nrf2/HO-1 signal pathway in ARPE-19 cells. Biomed Pharmacother. 2017;88:124-33. https://doi.org/10.1016/j.biopha.2016.11.089