• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.1801-1806
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• 2004

Stochastic nature of subgrid-scale stress causes the predictability problem in large eddy simulation (LES) by which the LES solution field decorrelates with field from filtered directnumerical simulation (DNS). In order to evaluate the predictability limit in a priori sense, the information on the interplay between resolved scale and subgrid-scale (SGS) is required. In this study, the analysis on the inter-scale interaction is performed by applying tophat and cutoff filters to DNS database of flow over a circular cylinder at Reynolds number of 3900. The effect of filter shape is investigated on the interpretation of correlation between scales. A critique is given on the use of tophat filter for SGS analysis using DNS database. It is shown that correlations between Karman vortex and SGS kinetic energy drastically decrease when the cutoff filter is used, which implies that the small scale universality holds even in the presence of the large scale coherent structure.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.631-638
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• 2008

Tandem affinity purification (TAP) method combined with LC-MS/MS is the most accurate and reliable way to study the interaction of proteins or proteomics in a genome-wide scale. For the first time, we used a TAP-tag as a mutagenic tool to disrupt protein interactions at the specific site. Although lots of commonly used mutational tools exist to study functions of a gene, such as deletional mutations and site-directed mutagenesis, each method has its own demerit. To test the usefulness of a TAP-tag as a mutagenic tool, we applied a TAP-tag to RNA polymerase II, which is the key enzyme of gene expression and is controlled by hundreds of transcription factors even to transcribe a gene. Our experiment is based on the hypothesis that there will be interrupted interactions between Pol II and transcription factors owing to the TAP-tag attached at the C-terminus of each subunit of Pol II, and the abnormality caused by interrupted protein interactions can be observed by measuring a cell-cycle of each yeast strain. From ten different TAP-tagged strains, Rpb7- and Rpb12-TAP-tagged strains show severe defects in growth rate and morphology. Without a heterodimer of Rpb4/Rpb7, only the ten subunits Pol II can conduct transcription normally, and there is no previously known function of Rpb7. The observed defect of the Rpb7-TAP-tagged strain shows that Rpb7 forms a complex with other proteins or compounds and the interruption of the interaction can interfere with the normal cell cycle and morphology of the cell and nucleus. This is a novel attempt to use a TAP-tag as a proteomic tool to study protein interactions.

• ALGAE
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• 제17권1호
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• pp.41-51
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• 2002

Three dominant rocky intertidal macroalgae, the fucoids Fucus gardneri and Pelvetiopsis limitata (Phaeophyta) and the red alga Mazzaella cornucopiae (= Iridaea cornucopiae) on the west coast of Vancouver Island, British Columbia, Canada were used in a series of field experiments to examine interspecific interactions. These experiments showed complex patterns which included an interchange of negative (inhibition) and positive (facilitation) interactions depending on neighbor distance. Less fucoid recruitment occurred in the plots with greater percent cover of a turfforming red alga, M. cornucopiae. However, experimentally removing Mazzaella turf (the turf was considered to be "blocking" fucoid recruits or "shading" growing recruits) did not increase recruitment. This result indicated that there may be another factor(s) involved in the survivorship of juvenile fucoids in the turf-removed plots. Morphological differences in adult plants between Mazzaella and the two fucoids resulted in another type of interaction; these began when fucoids successfully settled and grew nearby or within the red algal turf. By monitoring microhabitat at the individual plant level for two years, I found that survivorship of fucoid recruits showed different species-specific patterns. The patterns also varied as the microhabitat changed from Mazzaella turf edge to open space. For F. gardneri, longevity of P.limitata at all distances tested was similar. A reason for greater longevity of F. gardneri individuals at edge microhabitats may be that these sites have one side open to light and nutrients and another site that buffers them from desiccation and wave impact. In the Mazzaella-Fucus interaction, neighbor distance was a key factor in determining whether the outcome of the interaction would be competition or facilitation (or protection). This study provides experimental evidence that detectable biological interactions occur in this upper intertidal algal community where physical conditions are usually severe, and also indicates the importance of small scale examination in understanding macroalgal interactions in intertidal habitats.

• 의학교육논단
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• 제21권3호
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• pp.150-154
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• 2019

When standardized patients (SPs) are used for educational purposes, the authenticity of role play and the quality of feedback are essential requirements of SPs. This study was conducted to investigate medical students' assessment of SPs and to identify the components of SPs' performance that were most strongly correlated with patient-physician interaction score. One hundred and forty-two fourth-year medical students were asked to complete the Maastricht Assessment of Simulated Patients (MaSP) at the end of a clinical performance examination. SPs evaluated the patient-physician interactions using a 4-point Likert scale (1=poor to 4=excellent). Medical students' assessment of SPs using the MaSP was positively correlated with patient-physician interactions (r=0.325, p<0.01). Items addressing the authenticity of role play (e.g., "SPs appear authentic," "SPs might be real patients," and "SPs answer questions in a natural manner") were closely correlated with patient-physician interactions (p<0.001, p=0.027, and p=0.017, respectively). These results showed that the MaSP appears to be a useful instrument for evaluating SPs' performance and that the authenticity of SPs' performance was positively correlated with medical students' interactions. In order to improve patient-physician interactions, medical students should be given opportunities to practice their skills with SPs who have been trained to portray patients with a specific condition in a realistic way.

• Corrosion Science and Technology
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• 제16권2호
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• pp.64-68
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• 2017

The interactions between corrosion pits on stainless steel under loading conditions are studied by using a cellular automata model coupled with finite element method at a mesoscopic scale. The cellular automata model focuses on a metal/film/electrolyte system, including anodic dissolution, passivation, diffusion of hydrogen ions and salt film hydrolysis. The Chopard block algorithm is used to improve the diffusion simulation efficiency. The finite element method is used to calculate the stress concentration on the pit surface during pit growth, and the effect of local stress and strain on anodic current is obtained by using the Gutman model, which is used as the boundary conditions of the cellular automata model. The transient current characteristics of the interactions between corrosion pits under different simulation factors including the breakdown of the passive film at the pit mouth and the diffusion of hydrogen ions are analyzed. The analysis of the pit stability product shows that the simulation results are close to the experimental conclusions.

• 아동학회지
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• 제31권2호
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• pp.1-15
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• 2010

The purpose of this study was to analyze the teacher-child relationship as it is self-conceived by teachers in regards to children's peer-competence and self-regulation and to also examine how the teacher-child relationship affects peer-competence and self-regulation. 17 teachers were surveyed using the Student-Teacher Relationship Scale, Peer-Competence Scale and Self-Regulation Scale in regard to approximately 270 children (aged 4-5 years old). Our results indicated that the pro-sociality, sociality, and leadership qualities of child peer-competence correlated positively with the friendliness of teacher-child relationships but correlated negatively in cases of conflicts within the teacher-child relationship. The factors of self-appraisal, self-determination and behavior inhibition of child self-regulation were found to be positively related with the friendliness of teacher-child relation but related negatively with presence of the conflict within the teacher-child relationship. It was also found that sociable and self-determinative children tended to be on more friendly terms with teachers.

• Tribology and Lubricants
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• 제14권1호
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• pp.54-63
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• 1998

In many practical lubricated contacts such as a rough concentrated contact on the sliding of nominally flat surfaces, the fluid may be of molecular (nanometer) scale owing to the asperity interactions on the surfaces. Under this condition, there is insufficient lubricant on the concentrated contact spot to maintain a realistic continuum. Rheological behavior for this kind of concentrated contact has been studied extensively to know whether the application of viscous fluid model is appropriate. The interaction of two rough surfaces is simplified as perfectly flat-rough surfaces contact under certain conditions by "composite topography" and for a nanometer scale fluid film, three kinds of rheological fluid behavior are analyzed in elastohydrodynamic asperity point contact.t contact.

• Interaction and multiscale mechanics
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• 제2권1호
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• pp.15-30
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• 2009

The theoretical framework of the interaction fields for multiple scales based on field theory is applied to one-dimensional problem mimicking dislocation substructure sensitive intra-granular inhomogeneity evolution under fatigue of Cu-added steels. Three distinct scale levels corresponding respectively to the orders of (A)dislocation substructures, (B)grain size and (C)grain aggregates are set-up based on FE-RKPM (reproducing kernel particle method) based interpolated strain distribution to obtain the incompatibility term in the interaction field. Comparisons between analytical conditions with and without the interaction, and that among different cell size in the scale A are simulated. The effect of interaction field on the B-scale field evolution is extensively examined. Finer and larger fluctuation is demonstrated to be obtained by taking account of the field interactions. Finer cell size exhibits larger field fluctuation whereas the coarse cell size yields negligible interaction effects.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 1997년도 제26회 추계학술대회
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• pp.173-182
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• 1997

In many practical lubricated contacts such as a rough concentrated contact on the sliding of nominally flat surfaces, the fluid may be of molecular (nanometer) scale owing to the asperity interactions on the surfaces. Under this condition, there is insufficient lubricant on the concentrated contact spot to maintain a realistic continuum. Rheological behavior for this kind of concentrated contact was studied to know whether the application of viscous fluid model is appropriate. The interaction of two rough surfaces is simplified as perfectly flat-rough surfaces contact under certain conditions by "composite topography" and for a nanometer scale fluid film, three kinds of rheological fluid behavior are analyzed in elastohydrodynamic asperity point contact.t contact.

• 천문학회보
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• 제40권2호
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• pp.29.2-29.2
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• 2015

Interactions such as mergers and flybys play a fundamental role in shaping galaxy morphology. We used the Horizon Run 4 cosmological N-body simulations to study the aligments of spins and shapes of interacting haloes as a function of the halo mass and large-scale density. Interactions preferentially occur in the plane of rotation, and in the direction of the major axis of prolate haloes, and the trajectories are preferentially radial and prograde. We found a very strong alignment of the shapes already at redshift as high as 4. The spins are initially unaligned or even anti-aligned, and become more and more aligned as the redshift decreases. The alignment signals are stronger and evolve more at lower densities, and mass plays a secondary role.