• 한국식품저장유통학회지
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• 제9권4호
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• pp.380-384
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• 2002

창포의 뿌리를 주근(main root)과 측근(lateral root)으로 분리하여 3$0^{\circ}C$ 열풍건조분말의 주된 포화지방산으로는 caprylic acid, pentadecanoic acid, stearic acid 및 heneicosanoic acid 등 10종이 확인되었고, 이들은 각각 주근과 측근의 경우 caprylic acid가 28.35%, 31.44%이었고, 불포화지방산으로는 linoleic acid, palmitoleic acid 및 linolenic acid 등 6종이 확인되었고 이들 중 가장 높은 함유량을 보인 linoleic acid가 주근에 25.55%, 측근에 23.43%가 함유되어 있었다. 주요 유기산으로는 주근과 측근에 succinic acid, malic acid 및 citric acid 등으로 구성되어 있었고, 특히 succinic acid가 주근과 측근에서 각각 2,790 mg%, 2,630 mg%로 높은 함량을 함유하고 있었다. 무기질로는 K, Ca, p, Mg, Na, Fe, Zn, Mn, Cu 등 9종을 함유하고 있었으며, 창포뿌리의 주된 무기질로 확인된 K의 함량은 측근에 454 mg%로 주근의 194 mg% 보다 매우 높은 함량을 나타내었다. Ascorbic acid 함량은 주근과 측근에 각각 113.8 $\mu\textrm{g}$/g, 125.1 $\mu\textrm{g}$/g을 함유하고 있었고, 총페놀성 물질 함량은 주근에 463 mg%, 측근에 474 mg%로 매우 높은 수준이었으며 조사포닌의 함량은 주근에 2.51%, 측근에 3.39%로 높은 함량을 보였다.

• 생약학회지
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• 제50권4호
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• pp.305-311
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• 2019

The purpose of this study was to examine the changes in seasonal bioactive components of 4 and 6 years old fresh ginseng (Panax ginseng) and to provide basic information on the development of functional food using fresh ginseng. Seasonal changes were investigated by ginseng saponin analysis using HPLC method and acidic polysaccharides by carbazole sulfuric acid method. Total saponins showed the highest content of fresh ginseng collected in May, followed by March, July, and September. Fresh ginseng collected in May showed 2.5 times (4 years old) - 3.0 times (6 years old) higher than fresh ginseng collected in September. Acidic polysaccharides showed high content of fresh ginseng collected in March and September, and low content of fresh ginseng collected in May and July. From these results, the fresh ginseng collected in May can develop high concentrations of saponin. On the other hand, fresh ginseng collected in March and September is thought to be able to develop high concentration products of acidic polysaccharides.

• 생약학회지
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• 제42권4호
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• pp.361-365
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• 2011

The objective of this study is to provide basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve the proposed objective ginsenoside compositions of Black (BG) and Red (RG) ginseng extract with 95% ethyl alcohol were examined by means of HPLC. The crude saponin and ginsenoside composition of processed ginseng products were analyzed and compared, with BG topping the list with a crude saponin content of 7.53%, followed by RG (5.29%). Ginseng prosapogenin (ginsenosides $Rg_2$, $Rg_3$, $Rg_5$, $Rg_6$, $Rh_1$, $Rh_4$, $Rk_1$, $Rk_3$, $F_1$ and $F_4$) in BG was found to be contained almost 2.6 times as much as that in RG. Ginsenosides $Rg_3$, $Rg_5$, $Rk_1$, $Rh_4$ and $F_4$ in BG in particular were found to be almost 3 times as much as those in RG. $Rg_6$ and $Rk_3$ in BG were also found to be almost 4 times as much as those in RG.

• 생약학회지
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• 제48권3호
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• pp.255-259
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• 2017

This study was conducted to provide basic information on ginseng saponin of dried ginseng radices. In order to achieve the proposed objective ginsenoside compositions of dried ginseng radices extract with 70% ethyl alcohol were examined by HPLC. The total saponin content, the sum of all ginsenosides, showed that Wild simulated ginseng (WSG), White fine ginseng (WFG), Skin White ginseng (SWG), and White ginseng (WG) stood at 2.510%, 1.643%, 0.587, and 0.429%, respectively. WSG in PPD/PPT ratio was highest at 3.190, WFG (1.934), WG (1.600), SWG (1.386) in order. In the content of ginsenoside Rb1, one of the marker compounds of ginseng, WSG (1.095%) showed the highest content, and WFG (0.527%), SWG (0.246%), WG (0.133%) in this order. The content of ginsenoside Rb1 of WSG (1.095%) was 4.5 times higher than SWG (0.246%). WSG (0.230%) showed the highest content in ginsenoside Rg1, a marker compounds of ginseng, followed by WFG (0.180%), SWG (0.141%) and WG (0.086%). The content of ginsenoside Rg1 of WSG (0.230%) was 1.6 times higher than SWG (0.141%).

• 생약학회지
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• 제16권3호
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• pp.171-174
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• 1985

Panax ginseng root has been widely used as an important drug for thousands years in China, Korea and Japan. The main effective components of ginseng have been believed to be saponins. However, ginseng cultivation is very difficult and needs many years for growth. It has already been shown that Panax ginseng callus produces a considerable amount of the same kinds of saponins as in intact plants. Various culture conditions were examined for increased production of ginseng saponins by cell culture. The saponin contents and the growth rates in two cell lines of ginseng callus were compared in static and suspension cultures, rotary and reciprocal shaking cultures. It was shown that the growth rate in rotary shaking cultures of D5-B2K-B2K callus was the highest and ginseng saponin production was most effective in reciprocal cultures of D5-B2K-B2K callus. The saponin content per fresh weight of the culture was 1.03 times higher than that of the fresh ginseng root.

• Food Science and Biotechnology
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• 제18권2호
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• pp.513-518
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• 2009

This study was carried out to investigate the changes of yield, total phenolics, saponin content and composition, antimicrobial, and antioxidant activities of various fractions of fine ginseng root (Panax ginseng C.A. Mayer) by maceration method in the order of increasing polarity (hexane, chloroform, ethyl acetate, butanol, and water). Butanol fraction showed the highest total saponin content compare to other fractions. Hexane fraction could harvest significantly high ginsenoside Rg2, Rg1, and Rf (p<0.05). And the contents of ginsenoside Rh1, Rg3, and Rg1 showed relatively higher in the fraction of ethyl acetate than other fractions. The system of hexane-chloroform-ethyl aceate-butanol showed relatively high content of ginsenoside Re, Rd, Rc, Rb3, and Rb1. However, the last fraction of water still remained lots of Rb2 content. The fraction of water was the highest phenolics. The 1,1-diphenyl-2-picryhydrazil, superoxide, and hydroxyl radical scavenging activity of water fraction was higher than the other fractions. In antimicrobial activity, the fraction of hexane showed relatively high antimicrobial activity against Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, Bacillus cereus, and Escherichia coli. And the fractions of the chloroform and ethyl acetate showed higher antimicrobial activities than the other samples in against P. aeruginosa and S. typhimurium.

• Biomolecules & Therapeutics
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• 제2권4호
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• pp.316-321
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• 1994

This study was undertaken to estimate whether nalbuphine, a mixed agonist/antagonist opioid analgesic produced psychic dependence. Moreover, the physical dependence liability of nalbuphine was compared with that of morphine after 7 days administrations of the drugs in mice and rats, and the effects of ginseng total saponin (GTS) on the development of physical dependence on nalbuphine were also studied. Nalbuphine did not produce psychic dependence. However, various abstinence signs precipitated by naloxone were observed in nalbuphine-dependent mice and rats. As the nature of the dependence syndrome produced by nalbuphine 30 mg/kg under these conditions seems similar to that induced by morphine 10 mg/kg, it is clear that nalbuphine possesses the substantial abuse potential. Therefore, nalbuphine may be needed to initiate more stringent controls for the prevention of nalbuphine abuse. On the other hand, GTS inhibited the development of physical dependence on nalbuphine and reduced the contents of dopamine and its metabolite in the brains of mice. Accordingly, results of this study suggest that the inhibitory effects of GTS on the development of physical dependence on nalbuphine may involve dopaminergic mechanism. GTS may be useful for the therapy of physical dependence on nalbuphine.

• Natural Product Sciences
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• 제25권1호
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• pp.1-10
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• 2019

The ginsenoside content was compared with wild simulated ginseng (Panax ginseng) collected every season at 11 wild simulated ginseng plantations in Korea. As a result, the total saponin of 7 years old wild simulated ginseng showed the highest content of 4.5% in spring sampling wild simulated ginseng, 2.0% in summer sampling wild simulated ginseng, 1.2% in winter sampling wild simulated ginseng and 1.0% in autumn sampling wild simulated ginseng. And also, the total saponin of 10 years old wild simulated ginseng showed the highest content of 3.9% in spring sampling wild simulated ginseng, summer sampling wild simulated ginseng (1.8%), winter sampling wild simulated ginseng (1.6%) and autumn sampling wild simulated ginseng (0.6%). Therefore, the total saponin of spring sampling wild simulated ginseng was about 4.5 - 6.5 times higher than that of autumn sampling wild simulated ginseng regardless of cultivation period.

• 한국식품과학회지
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• 제46권6호
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• pp.769-772
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• 2014

도라지 건조 온도에 대한 기본 정보를 제공하고자 건조 온도에 따른 환원당 그리고 사포닌 함량을 조사한 결과를 요약하면 아래와 같다. Sucrose 함량은 $85^{\circ}C$에서 3,825 mg% $45^{\circ}C$에서 1,226 mg%로 건조 온도가 낮을수록 감소하였지만, glucose함량은 건조 온도가 높을수록 감소하였다. 본 시험에서 분석한 도라지 4종의 사포닌 중에 platycodin D 함량은 $85^{\circ}C$에서 197.5 mg%, $65^{\circ}C$에서는 334.7 mg%로 $45^{\circ}C$에서 956.0 mg%로 가장 높게 나타나 건조 온도가 낮을수록 증가하였으며, 고온(85) 건조에서 사포닌 중 polygalacin D의 함량이 가장 많았다. Sucrose와 사포닌 함량 간의 상관계수는 각각$-0.987^{**}$로 고도의 음의 상관을 보였다(p<0.01). 건조 온도 $45^{\circ}C$가 다른 건조 온도에 비해 색이 밝게 나타남을 확인할 수 있었다. 이상의 결과를 통하여 도라지의 건조 온도가 도라지의 사포닌 함량과 당 함량에 영향을 미치는 것을 확인할 수 있었으며, 도라지의 이용 용도에 따라 건조 온도를 달리 할 필요성이 있을 것으로 사료된다.

• 한국환경보건학회지
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• 제33권3호
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• pp.195-201
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• 2007

The objective of present study was to examine the composition of functional components (isoflavones, phytic acid, and saponin) in hypocotyls and cotyledons of six traditional Korean soybeans, namely Cheongtae, Seoritae, Jinjoori, Subaktae, Yutae, and Huktae. Three 'family' of isoflavones, as four chemical structures were present in hypocotyls and cotyledons of six soybean cultivars: the aglycones genistein, daidzein, and glycitein; the glycosides genistin, daidzin, and glycitin; the acetylglycosides 6"-O-acetylgenistin, 6"-O-acetyldaidzin, and 6"-O-acetylglycitin; and the malonylglycosides 6"-O-malonylgenistin, 6"-O-malonyldaidzin, and 6"-O-malonylglycitin. Isoflavone contents of hypocotyls and cotyledons differed among the cultivars, and glucosides and malonylglucosides accounted for more than 90% of the total phytoestrogens, with the remaining $1\sim7%$ taken up by aglycones. Concentrations of isoflavones in cotyledons were approximately about $10\sim20%$ of respective hypocotyls. Contents of phytic acids in hypocotyls and cotyledons of the selected soybean cultivars were $1.21\sim1.70%$ and $2.59\sim3.01%$, respectively. Hypocotyls of Seoritae showed the lowest content of phytic acid with 1.21%, while cotyledons of Cheongtae showed the highest content with 3.01%. The sapogenol concentrations ranged from $13.58mg/100g\sim20.82mg/100g$ for hypocotyls and $0.95mg/100g\sim2.55mg/100g$ for cotyledons showing that concentrations of saponin are $7\sim10$ times higher in hypocotyls than in cotyledons of respective soybeans. For both hypocotyls and cotyledons the sapogenol A were present in higher concentrations than soyasapogenol B.