The Comparison of Ginseng Saponin Composition and Contents in Dried Ginseng Radices

건삼류 생약의 인삼사포닌 성분 비교

  • Lee, Jae Bum (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Kim, Min Young (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Cho, Soon Hyun (Department of Pharmaceutical Quality Control, Daewon University College) ;
  • Ko, Sung Kwon (Department of Oriental Medical Food & Nutrition, Semyung University)
  • 이재범 (세명대학교 한방식품영양학부) ;
  • 김민영 (세명대학교 한방식품영양학부) ;
  • 조순현 (대원대학교 제약품질과) ;
  • 고성권 (세명대학교 한방식품영양학부)
  • Received : 2017.09.01
  • Accepted : 2017.09.15
  • Published : 2017.09.30

Abstract

This study was conducted to provide basic information on ginseng saponin of dried ginseng radices. In order to achieve the proposed objective ginsenoside compositions of dried ginseng radices extract with 70% ethyl alcohol were examined by HPLC. The total saponin content, the sum of all ginsenosides, showed that Wild simulated ginseng (WSG), White fine ginseng (WFG), Skin White ginseng (SWG), and White ginseng (WG) stood at 2.510%, 1.643%, 0.587, and 0.429%, respectively. WSG in PPD/PPT ratio was highest at 3.190, WFG (1.934), WG (1.600), SWG (1.386) in order. In the content of ginsenoside Rb1, one of the marker compounds of ginseng, WSG (1.095%) showed the highest content, and WFG (0.527%), SWG (0.246%), WG (0.133%) in this order. The content of ginsenoside Rb1 of WSG (1.095%) was 4.5 times higher than SWG (0.246%). WSG (0.230%) showed the highest content in ginsenoside Rg1, a marker compounds of ginseng, followed by WFG (0.180%), SWG (0.141%) and WG (0.086%). The content of ginsenoside Rg1 of WSG (0.230%) was 1.6 times higher than SWG (0.141%).

Keywords

References

  1. Namba, T. (1980) The Encyclopedia of Wakan-Yaku with Color Pictures (I), 1-5, Hoikusha, Osaka.
  2. Lee, D. C., Lee, M. O., Kim, C. Y. and Clifford, D. H. (1981) Effect of ether, ethanol and aqueous extracts of ginseng on cardiovascular function in dogs. Can. J. Comp. Med. 45: 182-187.
  3. Jie, Y. H., Cammisuli, S. and Baggiolini, M. (1984) Immunomodulatory effects of Panax ginseng C.A. Meyer in the mouse. Agents Actions. 15: 386-391. https://doi.org/10.1007/BF01972376
  4. Kim, Y. C., Kim, S. R., Markelonis, G. J. and Oh, T. H. (1998) Ginsenosides $Rb_1$ and $Rg_3$ protect cultured rat cortical cells from glutamate-induced neurodegeneration. J. Neurosci. Res. 53: 426-432. https://doi.org/10.1002/(SICI)1097-4547(19980815)53:4<426::AID-JNR4>3.0.CO;2-8
  5. Joo, C. N., Koo, J. D., Kim, D. S. and Lee, S. J. (1977) Biochemical studies of ginseng saponins. XI. The effects of ginseng saponins on alcohol dehydrogenase. Hanguk Saenghwa Hakhoe Chi 10: 109-120.
  6. Tahara, M., Kono, H., Mune, S. and Odashima, S. (1985) Action of ginsenosides on tumor cells. Growth inhibition and redifferentiation of neoplasia. Wakan Yaku Gakkaishi 2: 170-171.
  7. Yokozawa, T., Kobayashi, T., Oura, H. and Kawashima, Y. (1985) Studies on the mechanism of the hypoglycemic activity of ginsenoside-$Rb_2$ in streptozotocin-diabetic rats. Chem. Pharm. Bull. 33: 869-872. https://doi.org/10.1248/cpb.33.869
  8. Park, J. D. (1996) Recent studies on the chemical constituents of Korean ginseng. Korean J. Ginseng Sci. 20: 389-415.
  9. Shibata, S., Tanaka, T., Ando, T., Sado, M., Tsushima, S. and Ohsawa, T. (1966) Chemical studies on oriental plant drugs (XIV). Protopanaxadiol, a genuine sapogenin of ginseng saponins. Chem. Pharm. Bul. 14: 595-600. https://doi.org/10.1248/cpb.14.595
  10. Lee, C. R., Whang, W. K., Shin, C. G., Lee, H. S., Han, S. T., Im, B. O. and Ko, S. K. (2004) Comparison of ginsenoside composition and contents in fresh ginseng roots cultivated in Korea, japan, and China at various ages. Korean J. Food Sci. Technol. 36: 847-850.
  11. Jang, J. G., Lee, K. S., Kwon, D. W., Nam, K. Y. and Choi, J. H. (1983) Study on the changes of saponin contents in relation to root age of Panax ginseng. Korean J. Food & Nutrition 12: 37-40.
  12. Namba, T., Yoshizaki, M., Tomimori, T., Kobashi, K. and Mitsui, K. (1974) Chemical and biochemical evaluation of ginseng and related crude drugs. Yakugaku Zasshi 94: 252-260. https://doi.org/10.1248/yakushi1947.94.2_252
  13. Jo, H. K., Sung, M. C. and Ko, S. K. (2011) The comparison of ginseng prosapogenin composition and contents in Red and Black ginseng. Kor. J. Pharmacogn. 42: 361-365.
  14. Han, S. T., Shin, C. G., Yang, B. W., Hahm, Y. T., Sohn, U. D., Im, B. O., Cho, S. H., Lee, B. Y. and Ko, S. K. (2007) Analysis of ginsenoside composition of woods grown ginseng roots. Food Sci. & Biotechnol. 16: 281-284.
  15. Ko, S. K., Lee, K. H., Hong, J. K., Kang, S. A., Sohn, U. D., Im, B. O., Han, S. T., Yang, B. W., Chung, S. H. and Lee, B. Y. (2005) The change of ginsenoside composition in ginseng extract by the vinegar process. Food Sci. Biotechnol. 14: 509-513.
  16. Ko, S. K. and Im, B. O. (2009) The science of Korean ginseng, 47-52, Yakupsinmunsa, Seoul.