• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1841-1848
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• 2006

Insights into gene expression have the potential for improvement of antibiotic yield and the development of robust production hosts for use in recombinant biomolecule production. $Cubicin^{TM}$ (daptomycin for injection) is a recently approved antibiotic active against many Gram(+) pathogens, including those resistant to methicillin, vancomycin, and fluoroquinolones. Daptomycin is produced as a secondary metabolite by Streptomyces roseosporus. A 128 kb region of DNA including the daptomycin biosynthetic gene cluster (dpt) has been cloned. and sequenced. Using a selected array of nucleic acid probes representing this region, we compared the expression levels of the dpt genes between S. roseosporus wild-type (WT) and derived S. roseosporus high-producer of daptomycin (HP). We observed that the majority of the biosynthetic genes were upregulated in HP compared with WT; a total of 12 genes, including those encoding daptomycin synthetase, showed consistently and significantly higher expression levels, at least 5-fold, in HP compared with WT. In contrast, some genes, flanking the dpt cluster, were expressed at higher levels in the WT strain. The expression of housekeeping genes such as S. roseosporus rpsL, rpsG, and 16S (positive controls) and presumptive intergenic regions in the dpt cluster (negative control) were identical in the two strains. In addition, we compared transcription during the early, mid-log, and early-stationary phases of growth in the HP strain. The same set of genes was upregulated and downregulated under all conditions examined; housekeeping genes showed no relative change in expression level over the periods of growth tested. Analyses of this type would be of value in studies of strain improvement and also for the identification of gene regulation processes that are important for secondary metabolite production.

• Biomolecules & Therapeutics
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• v.16 no.3
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• pp.184-189
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• 2008

The proto-oncogene protein DEK has been implicated in various human disease including cancer. We have shown that DEK induces caspase-dependent apoptosis in Drosophila by regulating histone acetylation. Reverse transcription-polymerase chain reaction (RT-PCR) method based on annealing control primers was used to screen and identify differentially expressed genes (DEGs) in DEK overexpressed HeLa cells. Among the genes identified, clusterin and fibrillarin have major role in apoptosis pathway regulation. TFIIIC and RPS24 are implicated in HAT mediated transcriptional initiation and cololectal cancer, respectively. To further analyze DEK's role in apoptosis, multiplex PCR was performed. Caspase-3, -7, and -10 and proapoptotic gene bid were newly identified as possible target genes regulated by DEK expression.

• Korean Journal of Plant Taxonomy
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• v.41 no.3
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• pp.194-201
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• 2011

In order to acquire information on chloroplast DNA markers to evaluate the genetic diversity of evergreen broad leaved plants, we investigated the intraspecific variation of cpDNA in eight non-coding regions of nine species commonly distributed in East Asia. Although no variations were detected in psbA-trnH, rpoB-trnC, rpl16 and atpB-rbcL regions, a relatively large amount of intraspecific variations was detected in the psbC-trnS, rps16 and trnL-F regions. These results suggested that these three cpDNA markers are suitable to assess genetic diversity of the species investigated in this study. In contrast, intraspecific variations were detected in seven taxa except Hedera rhombea and Neolitsea aciculata. Neolitsea sericea and the taxa of Quercus had many polymorphic sites.

• Korean Journal of Plant Taxonomy
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• v.52 no.2
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• pp.85-96
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• 2022

The plant "Hong-do-go-deul-ppae-gi" has been considered as Crepidiastrum × muratagenii, a hybrid between C. denticulatum and C. lanceolatum, based on its morphological traits and geographical distribution. To reveal the hybrid origin of Hong-do-go-deul-ppae-gi, we examined additional morphological traits of this plant and its putative parents (C. denticulatum, C. lanceolatum, C. platyphyllum) and analyzed one nuclear ribosomal internal transcribed spacer (ITS) region and four chloroplast regions (trnT-L, trnL-F, rpl16 intron, and rps16 intron). As a result of examining the morphological traits, putative hybrid individuals were classified into three types based on the habit, cauline leaf, outer phyllary, and achene beak traits. A molecular analysis found that the ITS sequences of Type 1 and Type 2 individuals showed additive species-specific sites of C. denticulatum and C. lanceolatum. Plastid sequences of Type 1 and Type 2 individuals showed C. denticulatum and C. lanceolatum sequences, respectively. However, Type 3 individuals had ITS and plastid sequences corresponding to C. denticulatum. Accordingly, Type 1 and Type 2 individuals not only share morphological traits with C. denticulatum and C. lanceolatum but also show additive species-specific sites for C. denticulatum and C. lanceolatum, and not C. platyphyllum, supporting its origin as a hybrid between C. denticulatum and C. lanceolatum. Type 3 had morphological traits similar to other hybrid types but was distinguished with respect to outer phyllaries and demonstrated some resemblance to C. denticulatum. In a molecular analysis, Type 3 was found to be identical with regard to the sequence of C. denticulatum and was judged to be an ecological variation of C. denticulatum.

• The Optical Journal
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• s.122
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• pp.35-37
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• 2009

대한사진영상신문사가 주최하는 2009 디지털포토포럼 및 사진기자재전 기자 간담회가 지난 6월16일 신도림 테크노마트 11층 기자회견실에서 열렸다. 이번 기자 간담회에서는 대한사진영상신문사, 광학세계, 사진예술, 디시인사이드, 다나와, 디지털캐치 등의 기자들이 참석한 가운데 한국후지필름(주)의 신개념 포토 솔루션 '후지 포토매니저 시스템', 송화시스템기술(주)의 포토북 제작 솔루션 '픽토리', 한국HP(주)의 디지털 포토 솔루션 'HP RPS' 등 각 제품 소개와 공급사의 마케팅 전략 발표 시간을 가졌다. 아울러 국내 사진시장의 환경변화에 따른 스튜디와 및 미니랩 현상소의 대응 전략을 발표하는 세미나장과 사진기자재 업체들의 관련 제품 전시장을 둘러보는 시간을 가졌다.

• IEMEK Journal of Embedded Systems and Applications
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• v.7 no.4
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• pp.173-179
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• 2012

In this paper, we propose an efficient architecture for 2D IWT using an existing 1D IWT. Lifting based IWT is the architecture of which a multiplier is replaced by adders and shift registers. The structure is relatively simple and modular. The proposed architecture to process an image size with 256x256 pixels consists of 16 adders, 8 shift registers, and some memories. By processing two rows at the same time, 2D sub-band coefficients can be calculated immediately after 1D sub-band coefficients have been processed. The architecture is designed so that each image can be inputted consecutively. The number of adders and shift registers is increased by twice comparing the existing architecture, but the memory size and the execution time are decreased by half. The proposed architecture is implemented using Verilog-HDL and simulated using iSim. It is synthesized and demonstrated at ISE for xc5vlx330 in RPS3K board.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.67 no.1
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• pp.16-21
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• 2018

As a result of the Fukushima disaster and climate change due to excessive greenhouse gases, international energy affairs are currently focused on establishing safe and environment-friendly policies. To achieve this, Republic of Korea has established a plan for environment-friendly energy supplies. It is expected that policy enforcement will be accompanied by an increase in energy supply costs. An analysis of energy supply costs is necessary before the establishment of any national energy policy. This paper analyzes and compares the energy supply costs accompanying environmental and nuclear energy policies, based on the Korean National Energy Master Plan and the Basic Plan for Long-Term Electricity Supply and Demand, in order to understand the implications of these national energy policies.

• The Plant Pathology Journal
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• v.27 no.4
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• pp.301-309
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• 2011

The filamentous fungus Fusarium graminearum is an important cereal pathogen. Although quantitative realtime PCR (qRT-PCR) is commonly used to analyze the expression of important fungal genes, no detailed validation of reference genes for the normalization of qRT-PCR data has been performed in this fungus. Here, we evaluated 15 candidate genes as references, including those previously described as housekeeping genes and those selected from the whole transcriptome sequencing data. By a combination of three statistical algorithms (BestKeeper, geNorm, and NormFinder), the variation in the expression of these genes was assessed under different culture conditions that favored mycelial growth, sexual development, and trichothecene mycotoxin production. When favoring mycelial growth, GzFLO and GzUBH expression were most stable in complete medium. Both EF1A and GzRPS16 expression were relatively stable under all conditions on carrot agar, including mycelial growth and the subsequent perithecial induction stage. These two genes were also most stable during trichothecene production. For the combined data set, GzUBH and EF1A were selected as the most stable. Thus, these genes are suitable reference genes for accurate normalization of qRT-PCR data for gene expression analyses of F. graminearum and other related fungi.

• Korean Journal of Plant Taxonomy
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• v.51 no.1
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• pp.109-114
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• 2021

Veronica nakaiana Ohwi (Plantaginaceae) is an endemic taxon on Ulleungdo Island, Korea. We report the second complete chloroplast genome sequence of V. nakaiana. Its genome size is 152,319 bp in length, comprising a large single-copy of 83,195 bp, a small single-copy of 17,702 bp, and a pair of inverted repeat regions of 25,711 bp. The complete genome contains 115 genes, including 51 protein-coding genes, four rRNA genes, and 31 tRNA genes. When comparing the two chloroplast genomes of V. nakaiana, 11 variable sites are recognized: seven SNPs and four indels. Two substitutions in the coding regions are recognized: rpoC2 (synonymous substitution) and rpl22 (nonsynonymous substitution). In nine noncoding regions, one is in the tRNA gene (trnK-UUU), one is in the intron of atpF, and seven are in the intergenic spacers (trnH-GUG~psbA, trnK-UUU, rps16~trnQ-UUG, trnC-GCA~petN, psbZ~trnG-GCC, ycf3~trnS-GGA, ycf4~cemA, and psbB~psbT). The data provide the level of genetic variation in V. nakaiana. This result will be a useful resource to formulate conservation strategies for V. nakaiana, which is a rare endemic species in Korea.

• The Journal of the Acoustical Society of Korea
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• v.16 no.4
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• pp.57-63
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• 1997

In this paper, we study the efficient feature vector extraction method and front-end processing to improve the performance of the speech recognition system using KT(Korea Telecommunication) database collected through various telephone channels. First of all, we compare the recognition performances of the feature vectors known to be robust to noise and environmental variation and verify the performance enhancement of the recognition system using weighted cepstral distance measure methods. The experiment result shows that the recognition rate is increasedby using both PLP(Perceptual Linear Prediction) and MFCC(Mel Frequency Cepstral Coefficient) in comparison with LPC cepstrum used in KT recognition system. In cepstral distance measure, the weighted cepstral distance measure functions such as RPS(Root Power Sums) and BPL(Band-Pass Lifter) help the recognition enhancement. The application of the spectral subtraction method decrease the recognition rate because of the effect of distortion. However, RASTA(RelAtive SpecTrAl) processing, CMS(Cepstral Mean Subtraction) and SBR(Signal Bias Removal) enhance the recognition performance. Especially, the CMS method is simple but shows high recognition enhancement. Finally, the performances of the modified methods for the real-time implementation of CMS are compared and the improved method is suggested to prevent the performance degradation.