• 한국임상수의학회지
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• 제35권5호
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• pp.175-177
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• 2018

The objective of this polymerase chain reaction (PCR)-based research was to determine the prevalence of vector-borne pathogens in stray cats in Yangju and Gwacheon cities, South Korea. Total 50 stray cats were sampled for this PCR-based survey; 33 samples and 17 samples were collected from Yangju and Gwacheon cities, respectively. Total positive presence rates were 6%, 6% and 24% for hemotropic mycoplasmas, Rickettsia spp. and Babesia spp., respectively in this study. Babesia spp. was the predominant pathogen present in the stray cats of both cities followed by hemotropic mycoplasmas and Rickettsia spp. It is recommended that a large-scale study of the prevalence of infectious agents among stray cats should be undertaken in all regions of South Korea.

• 대한바이러스학회지
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• 제28권4호
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• pp.377-382
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• 1998

Hemorrhagic fever with renal syndrome (HFRS), scrub typhus, murine typhus and leptospirosis have been the principal acute febrile diseases in Korea. To evaluate the seroepidemiologic patterns of acute febrile illness, sera collected from 2,423 patients in 1996 were examined for antibodies against Hantaan virus, Orientia tsutsugamushi, Rickettsia typhi, and Borrelia burgdorferi by indirect immunofluorescent antibody technique (IFA) and macroscopic agglutination test for Leptospira interogans. Seropositive cases against Otsutsugamushi, Rickettsia typhi, Leptospira interogans and Hantaan virus were 192 (7.9%), 193 (8.0%), 12 (0.5%) and 324 (13.4%), respectively. Male was more affected in HFRS and murine typhus contrasting to scrub typhus and leptospirosis in female. Most positive cases occurred during October and November for scrub typhus, and during November and December for HFRS. These results showed similar patterns with previous epidemical data for recent couple of years, and possibly implied no significant changes occurred in ecologic situations for acute febrile diseases in Korea.

• 한국동물위생학회지
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• 제39권1호
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• pp.29-34
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• 2016

This study was carried out to investigate the tick-borne pathogens from one hundred nineteen cattle farms (38 farms of Andong, 41 of Yeongju, 12 of Uiseong, 5 of Cheongsong, 5 of Yoengyang, 18 of Bonghwa) in northern areas of Gyeongbuk province by polymerase chain reaction (PCR). Among 119 cattle farms, the positive ratios against Babesia, Theileria, Anaplasma, Ehrlichia and Rickettsia were 3.4% (4/119), 10.1% (12/119), 6.7% (8/119), 1.7% (2/119) and 16.8% (20/119), respectively. Also, the PCR results revealed that 8 farms were positive for T. sergenti in positive of Theileria and 2 farms were positive for A. phagocytophilum in positive of Anaplasma. Therefore, further studies regarding vectors, environmental condition, interaction between domestic and wild animals and development of control program are needed to reduce the numbers of bovine tick-borne disease in northern areas of Gyeongbuk province.

• Parasites, Hosts and Diseases
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• 제59권5호
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• pp.489-496
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• 2021

Ticks can transmit pathogenic bacteria, protozoa, and viruses to humans and animals. In this study, we investigated the microbiomes of Haemaphysalis longicornis according to sex and life stages. The Shannon index was significantly higher for nymphs than adult ticks. Principal coordinates analysis showed that the microbiome composition of female adult and male adult ticks were different. Notably, Coxiella-like bacterium (AB001519), known as a tick symbiont, was found in all nymphs and female adult ticks, but only one out of 4 male adult ticks had Coxiella-like bacterium (AB001519). In addition, Rickettsia rickettsii, Coxiella burnetii, and Anaplasma bovis were detected in this study.

• Parasites, Hosts and Diseases
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• 제29권2호
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• pp.181-188
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• 1991

최근 전국적으로 높은 이환률을 보이고 있는 쭈쭈가무시병에 대한 방제 대책이 시급한 현실에 반해 본 질병에 대한 연구가 거의 이루어져 있지 않기 때문에 저자들은 1989년 7월부터 1950년 9월까지 9개 지역에서 숙주동물에 대한 역학적 조사를 실시하여 다음과 같은 결과를 얻었다. 1. 모두 794개의 Sherman식 live trap을 설치하여 111마리의 들쥐로 포획하여 trapping rate가 평균 14.0%이었다. 계절별로 보면 봄, 여름, 가을에 각각 11.7%, 11.2%, 12.8%이었고 겨울에는 24.0%로 가장 높았다. 포획한 111마리 중 103마리가 등줄쥐 (Apodemus agrarius)로 92.8%를 차지하여 우점종이었고, 땃쥐 (Crocidura Zasiura)가 7마리로 6.3%이었으며. 갈밭쥐 (Microtns fortis)는 1마리가 채집되어 0.9%이었다. 들쥐의 털진드기 기생률을 보면, 등줄쥐의 경우 103마리 중 84마리로 81.6%이었고, 땃쥐의 경우 7마리 중 2마리로 28.6%이었다. Chigger index는 등줄쥐의 경우 43.0개체였고 만취는 0.9개체였다. 2. 등줄쥐 혈청의 Rickettsia tsutsugamushi 항체 양성률을 보면 평균 41.8%이었는데, 조사 지역에 따라 큰 차이가 있어 고양군 도래 5리 54.9%, 고양군 고양리 20%, 고양군 원홍 2리 28.6%, 청인군 시목리 40%이었고 고양군 행신 1리, 파주군 광탄 3리 와 예산군 덕산리에서는 모두 음성이었다. 땃쥐의 경우는 7마리 모두 음성이었다. 동일 장소에서의 등줄쥐 혈청의 R. tsutsugamushi 항체 양성률의 계절적 변동은 11월부터 3웜까지는 평균 75.8%이었는데 4일에는 30.3%로 감소하였고 5월부터 8월에 걸쳐 평균 13.3%로 크게 감소하였다. 조사한 모든 양성 혈청의 항체가를 보면 Karp, Gilliam및 Kato 주에 대해 서로 교차반응을 나타냈는데 Karp주와 Kato 주 간에 보다 강한 교차반응을 보였다. 3. 총 94마리의 등줄쥐로부터 마우스 복강접종법과 간접 면역형광법으로 R. tsutsugamushl치 분리를 시도한 바 3마리에서 분리되어 감염률은 3.2%이었다.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.653-659
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• 2015

The objective of this study was to investigate the prevalence of tick-borne pathogens in the Korean water deer (Hydropotes inermis argyropus). Pathogens were identified using PCR which included Anaplasma, Ehrlichia, Rickettsia, and Theileria. Rickettsia was not detected, whereas Anaplasma, Ehrlichia, and Theileria infections were detected in 4, 2, and 8 animals, respectively. The most prevalent pathogen was Theileria. Of the 8 Theileria-positive animals, 2 were mixed-infected with 3 pathogens (Anaplasma, Ehrlichia, and Theileria) and another 2 animals showed mixed-infection with 2 pathogens (Anaplasma and Theileria). Sequencing analysis was used to verify the PCR results. The pathogens found in this study were identified as Anaplasma phagocytophilum, Ehrlichia canis, and Theileria sp. To the best of our knowledge, this is the first report identifying these 3 pathogens in the Korean water deer. Our results suggest that the Korean water deer may serve as a major reservoir for these tick-borne pathogens, leading to spread of tick-borne diseases to domestic animals, livestock, and humans. Further studies are needed to investigate their roles in this respect.

• Parasites, Hosts and Diseases
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• 제56권6호
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• pp.589-596
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• 2018

Ticks are the vectors of various pathogens, threatening human health and animal production across the globe. Here, for the first time we detected Ricketssia spp., Borrelia spp. and protozoan in ticks from Poyang Lake region in Jiangxi Province of eastern China. In 3 habitat categories and on 12 host species, 311 ticks from 11 species were collected. Haemaphysalis longicornis was the predominant species, accounting for 55.63%, followed by Rhipicephalus microplus, Haemaphysalis flava and Ixodes granulatus. Of the collected ticks, 7.07% were positive for tick-borne pathogens, and H. longicornis and H. flava were found to be co-infected with Ricketssia spp. and protozoan. H. flava was the most detected positive for tick-borne pathogens, whereas H. longicornis had the lowest infection rate, and the difference in infection rates between tick species was significant (${\chi}^2=61.24$, P<0.001). Furthermore, adult ticks demonstrated remarkably greater infection rate than immature ticks (${\chi}^2=10.12$, P=0.018), meanwhile ticks on Erinaceidae showed significantly higher positivity than ticks collected on other host species (${\chi}^2=108.44$, P<0.001). Genetic fragment sequencing and analyses showed at least 4 pathogen species presence in ticks, namely Borrelia yangtzensis, Rickettsia slovaca or Rickettsia raoultii related genospecies, Babesia vogeli and Hepatozoon canis or Hepatozoon felis related genospecies. The finding indicates that the abundant ticks can carry diverse pathogens in Poyang Lake region, and pathogen infection is highly related to species, vertebrate hosts and life stages of ticks.

• Journal of Medicine and Life Science
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• 제15권2호
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• pp.84-88
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• 2018

The aim was to evaluate different results from using crude incidence rate (CIR) instead of agestandardized incidence rate (AIR) when comparing groups having different age structures. After selecting a report using CIRs, AIRs and their 95% confidence intervals(CI) was calculated from the raw data. The statistically significant difference between CIR and AIR was decided based on AIR's 95% CI. Comparing with CIRs, AIRs were under-estimated. In addition, there were no statistical significance of annual trends(P=0.59). These findings are an additional evidence using AIR when comparing level of occurring infectious diseases on groups with different age structures.

• 한국군사과학기술학회지
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• 제22권4호
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• pp.575-580
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• 2019

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

• Tuberculosis and Respiratory Diseases
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• 제52권4호
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• pp.367-375
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• 2002

연구배경: 발진열은 벼룩에 의해 매개되며 전세계적으로 발생되는 리케치아질환으로 대개 경한 임상경과를 보이나 간혹 중증으로 발현된다. 저자들은 발진열로 유발된 급성호흡곤란증후군 6예를 경험하게 되어 그 임상상을 분석하고자 하였다. 방 법: 1991년 이후 서울중앙병원에 발열을 주소로 내원하여 발진열로 진단된 환자들을 찾아 호흡곤란증후군을 동반한 군과 그렇지 않은 군으로 나누어 각군에 대한 연령, 성별, 혈액학적 검사 및 미생물학적 검사에 대해 조사하여 비교하였다. 발진열의 진단은 특이적 면역형 광항체검사법을 시행하여 초회 항체역가가 1:512 이상이거나 추적 항체가의 4배이상 증가가 있으면서 발진열에 합당한 임상양상이 있는 경우로 한정하였고 급성호흡곤란증후군은 American-European Consensus Conference에 제시된 기준에 따라 정의하였다. 결 과: 호흡곤란증후군이 동반된 6례를 포함 모두 15예가 있었다. 호흡곤란증후군이 동반된 6명의 환자 중 2명이 여자였고 3명이 도시에 거주하였다. 발진은 모두에서 관찰되지 않았다. 한명이 치료도중 사망하였다. 급성호흡곤란증후군과 연관된 인자로 진단 및 치료시까지의 지연시간과 저알부민혈증, 혈중 bilirubin의 증가, 높은 APACHE III 점수와 MOD 점수가 통계적 유의성을 보였다. 결 론: 발진열은 풍토병의 성격을 띤 지역에서 원인미상의 급성호흡곤란증후군의 한 원인으로 고려될 수 있고 최근 도시거주자에게도 발병되고 있다. 발진열에 의한 급성호흡곤란증후군의 예후는 다른 원인에 의한 경우보다 좋지만 조속한 진단 및 치료가 중요할 것으로 생각된다.