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http://dx.doi.org/10.9766/KIMST.2019.22.4.575

Development and Validation Study of Biological Agent Detection Kit  

Joe, Hae Eun (The 4th Research and Development Institute, Agency for Defense Development)
Publication Information
Journal of the Korea Institute of Military Science and Technology / v.22, no.4, 2019 , pp. 575-580 More about this Journal
Abstract
In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.
Keywords
Real-Time RT-PCR Kit; Biological Agents; Detection;
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Times Cited By KSCI : 1  (Citation Analysis)
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