• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 추계학술대회
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• pp.227-227
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• 2017

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 춘계 학술대회지
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• pp.132-133
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• 1999

• Journal of Plant Biology
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• 제31권1호
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• pp.41-49
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• 1988

Several cultivars of rice were examined for induction of embryogenic callus on a medium containing MS salts, vitamins and 2, 4-D under darkness. Embryogenic callus was obtained from cultivar Cheonma with high ratio and embryo-like structures were formed from the callus on a medium with or without reduced 2, 4-D. Somatic embryoids with a plumule and radicle axis surrounded by a scutellum were observed. These embryoids germinated and produced plantlets in 30 days on the same medium. Protoplasts isolated from an embryogenic cell suspension culture derived from embryogenic callus were cultured either in liquid or in agar medium and protoplast derived cell colonies were obtained in 3-4 weeks.

• Journal of Biosystems Engineering
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• 제34권6호
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• pp.439-445
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• 2009

This study was performed to investigate the optimum abrasive and friction milling ratio. This was accomplished by determining changes in the quality, such as whiteness, moisture content, broken kernel, unstripped embryo rate, and surface characteristics or milling difference, during an abrasive and friction based milling process. When only abrasive was milled, the increase of whiteness was fast in the first milling, whereas the increasing rate of whiteness was small in the latter milling. The decreasing rate of moisture content and broken kernel increased as the friction milling ratio was increased. Combining with the friction milling was considered a suitable method because the unstripped embryo rate was high only when abrasive milling was used. In the case of a high abrasive milling ratio, a significant milling difference was observed in the initial milling. This indicated that the milling difference was not completely eliminated despite using friction milling in the latter milling. Consequently, it was necessary to minimize the milling difference in the initial milling. When milling quality was synthetically considered, the abrasive milling ratio was varied from 20~50%. When the abrasive milling ratio was greater than 40%, the external quality of the rice milled deteriorated since holes and defects generated on the surface in the initial milling were not removed. Due to this deterioration in surface characteristics, an abrasive milling ratio of 30% was identified as a suitable level.

• 식물조직배양학회지
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• 제22권3호
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• pp.143-148
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• 1995

벼의 뿌리조직을 기내배양하여 배발생캘러스의 형성과 식물체 재분화율의 품종간 차이 및 완숙배와 뿌리조직에서 유래된 캘러스의 현탁배양 기간별 부정배 형성정도와 식물체 재분화율 등에 대한 몇가지 실험을 수행하여 얻어진 결과를 요약하면 다음과 같다. 벼 뿌리조직으로부터 캘러스 형성 및 식물체 재분화 능력의 품종간 차이는 뚜렷하게 나타났으며, 자포니카형 품종들이 통일형 품종에 비해 캘러스의 생장량도 많고 식물체 재분화율도 높은 경향이었고, 공시품종 중 "영덕벼"의 식물체 재분화율이 13%로 가장 높았다. 뿌리 및 현미배양에서 형성된 캘러스를 고체배지에서 2 주 간격으로 7회 계대배양한 바, 계대배양 횟수가 증가됨에 따라 식물체 재분화율이 증가되었다가 5회째부터는 점차 감소하였다. 뿌리조직에서 형성된 캘러스의 현탁배양에서 모양이 둥근세포와 그들의 세포괴는 배양 24일 후에 최대치를 나타내었고 그 이후는 감소하였다. 현탁배양 기간별 뿌리조직에서 형성된 캘러스의 체세포배 형성률은 배양 90일까지는 배양기간이 길어질 수록 증가하였으나, 그 이후는 감소하였고, 식물체 재분화율도 배양기간이 길어질수록 감소하는 경향이었다.소하는 경향이었다.

• Journal of Plant Biotechnology
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• 제30권3호
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• pp.281-291
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• 2003

In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is the most prevalent technique to rapidly identify a large number of proteome analysis. However, the conventional Western blotting/sequencing technique has been used in many laboratories. As a first step to efficiently construct protein cata-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein sports are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins(i, e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 45% of total rice cDNA have been deposited in the EMBL database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that tuned out to be calreticulin, gibberellin-binding protein, which is ribulose-1.5-bisphosphate carboxylase/oxygense active in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins(http://genome.c.kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Also, the information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful be in the plant molecular breeding.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2004년도 춘계 학술대회지
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• pp.12-27
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• 2004

Thanks to spectacular advances in the techniques for identifying proteins separated by two-dimensional electrophoresis and in methods for large-scale analysis of proteome variations, proteomics is becoming an essential methodology in various fields of plant sciences. Plant proteomics would be most useful when combined with other functional genomics tools and approaches. A combination of microarray and proteomics analysis will indicate whether gene regulation is controlled at the level of transcription or translation and protein accumulation. In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is a most prevalent technique to identify rapidly a large of proteins in proteome analysis. However, the conventional Western blotting/sequencing technique us still used in many laboratories. As a first step to efficiently construct protein data-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein spots are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins (i. e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 30% of total rice cDNA have been deposited in the database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that fumed out to be calreticulin, gibberellin-binding protein, which is ribulose-1,5-bisphosphate carboxylase/oxygenase activate in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins (http://genome .c .kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Recently, we are separated proteins from grain filling and seed maturation in rice to perform ESI-Q-TOF/MS and MALDI-TOF/MS. This experiment shows a possibility to easily and rapidly identify a number of 2-DE separated proteins of rice by ESI-Q-TOF/MS and MALDI-TOF/MS. Therefore, the Information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful in the plant molecular breeding. Also, information from our study could provide a venue to plant breeder and molecular biologist to design their research strategies precisely.

• Applied Biological Chemistry
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• 제47권1호
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• pp.61-66
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• 2004

신선찰거대배아미, 화청거대배아미 및 남풍거대배아미 등 거대배 돌연변이 계통 쌀 3종류 및 일반미의 70% 에탄올 추출물을 제조하여, 이들의 항산화 활성 및 항변이원성을 비교 검정하였다. 거대배아미 추출물의 항산화 활성은 DPPH radical 및 Fenton 반응에 의해서 유도되는 hydroxy radical의 소거활성, hypoxanthine/xanthine oxidase system에서 생성되는 활성산소종인 superoxide radical의 소거활성, linoleic acid자동산화에 대한 지질 과산화 억제활성 및 토끼 적혈구 막지질의 과산화 억제활성 둥으로써 검정하였으며, 항변이원성은 E. coli PQ 37 균주를 사용하여 화학적 변이원 mitomycin C에 대한 변이원성 억제효과를 SOS chromotest에 의해서 검정하였다. 일반미 품종에 비해서 거대배아미 품종의 항산화 활성 및 항변이원성이 모두 높았으며, 거대배아미 품종 중에서는 남풍거대배아미가 가장 효과적인 경향이 있었다. 남풍거대배아미의 DPPH radical, superoxide radical과 hydroxyl radical 소거활성, 그리고 지질과산화 억제활성은 일반미보다 각각 2.3배, 3.3배, 1.7배 및 2.5배 정도까지 더 높았다.

• Journal of Plant Biology
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• 제38권1호
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• pp.55-62
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• 1995

벼(Oryza sativa L. cv. Kye Hwa) 성숙종자에서 유도한 배발생 세포(embryogenic cell, EC)의 동위효소 발현양상과 효소활성을 조사하였다. 배발생 캘러스로부터 확립된 EC 현탁배양은 세포가 둥글며 세포질이 충만한 세포들로 이루어졌으나 비배발생 세포(nonembryogenic cell, NEC)의 현탁배양은 크게 신장한 액포화된 세포들로 구성되었다. 이러한 EC와 NEC의 peroxidase, esterase, acid phosphatase 그리고 malate dehydrogenase의 동위효소 양상과 활성도를 분석한 결과 밴드의 수와 특이성 그리고 밴드 활성 등에 현저한 차이를 보여주었으며 또한 이들 동위효소의 효소 활성도 측정 결과 EC에서 훨씬 더 높게 나타났다. NEC에는 나타나지 않은 EC의 특이 밴드와 강한 밴드 활성 그리고 높은 효소 활성 등은 EC의 형태적, 분화능과의 관련성을 내포하고 있다. 이러한 결과들은 이들 효소의 특이 밴드들이 벼에서 EC의 생화학적 표지자로서 사용될 수 있음을 내포하고 있다.

• 한국응용곤충학회지
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• 제23권2호
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• pp.126-131
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• 1984

1. 영남작물시험장, 호남작물시험장 및 충남농촌진흥원에서 분양받은 18개의 벼종자시료와 충남지역 일반농가에서 수확한 8개의 벼종자시료 등 모두 26개의 종자시료를 공시하여 표준습지법으로 조사하였던 바 21종의 균류가 검출되었으며, 벼 갈샐잎마름병균(Gerlachia oryzae)은 22개의 벼 종자시료에서 $1.0\~45.0\%$의 범위로 검출되었다. 2. 종자전염성 G. oryzae의 효과적인 검출방법을 찾기 위하여 표준습지법, 냉동습지법, 물한천법 등으로 비교 조사한 결과, 냉동습지법에서 검출율이 가장 높았다. 3. 벼종자의 각 부위를 무균적으로 분리하여 부위별로 G. oryzae의 감염여부를 표준습지법으로 조사한 결과, 벼 종자의 껍질에서 가장 많이 검출되었으며, 심하게 이병된 종자는 배유 및 종피 뿐 만 아니라 배에 까지 감염되어 있었다. 4. G. oryzae에 감염된 벼 종자를 파종하여 심하게 이병된 종자는 종자부패 및 묘입고를 일으켰으며, 약하게 이병된 종자는 초엽, 1엽, 2엽에 갈색의 병징감염을 초래하였다.