• 운동영양학회지
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• 제13권1호
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• pp.45-50
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• 2009

This study evaluated the effects of D-ribose supplementation on exercise time in a hypoxic chamber. Eight healthy young males participated in experiments under all four different conditions; placebo+normoxic, placebo+hypoxic, ribose+normoxic, and ribose+hypoxic. Subjects took 1 g per 10 kg body weight of ribose dissolved in drinking water 30 minutes before and immediately before running. We observed the run-to-exhaustion time, the maximum heart rate, and the changing pattern of the heart rate during exercise. The longest running time was achieved when subjects ran under normoxic condition with ribose supplementation. The shortest running time occurred when subjects ran under hypoxic conditions without ribose supplementation. We measured MDA and GPx to determine any changes in oxidative stresses or antioxidative systems. MDA was affected by the environmental conditions and the running time. The activity of GPx showed a significant difference only with the different environmental conditions of exercise. The results of this study indicate that ribose can be considered a possible ergogenic during exercise in specific conditions, but more detailed and well-controlled studies are needed to make a definitive conclusion.

• KSBB Journal
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• 제23권1호
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• pp.31-36
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• 2008

The use of L-carbohydrates and their corresponding nucleosides in medicinal application has greatly increased. For example L-ribose has been much in demand as the starting material for curing hepatitis B. High performance liquid chromatography (HPLC) method was studied for the analysis of ribose and arabinose fractions from ion exchange chromatography (IEC). Dowex Monosphere 99 Ca/320 resin was packed in IEC to separate ribose and arabinose under various operating conditions. $NH_{2}$ and sugar HPLC columns were then used to analyze the fractions from the IEC column. Pulse input method (PIM) was also used to measure adsorption isotherms of ribose and arabinose in the Dowex column and HPLC columns. Experimental results and simulations by ASPEN chromatography were compared with fair agreement.

• KSBB Journal
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• 제23권2호
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• pp.135-141
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• 2008

SMB 크로마토그래피를 위한 예비 실험으로서 L-ribose와 L-arabinose의 혼합물의 분리 특성을 $NH_2$ 컬럼을 사용하여 확인하였고 두 성분 분리 최적 조건으로 Acetonitrile과 증류수의 조성이 85/15 (v/v)에서 실험을 수행하였다. PIM(Pulse input method)과 Aspen chromatography 전산모사를 통해 L-ribose와 L-arabinose가 선형 흡착등온식을 따르는 것을 알 수 있었으며 각 성분의 Henry상수를 추산하였고 다음과 같다. $$C_{S,Ribose}=3.51{\cdot}C_{M.Ribose}$$ $$C_{S,Arabinose}=5.07{\cdot}C_{M.Arabinose}$$ 이를 바탕으로 SMB 크로마토그래피의 최적 운전조건을 수립하기 위하여 Triangle 이론에 의한 운전 조작변수를 추산함으로서 $m_2\;=\;3.51$, $m_3\;=\;5.07$일 때 즉, 삼각형에서 꼭지점의 조건에서 L-ribose와 L-arabinose의 순도가 각각 85, 80% 정도로 두 성분의 분리에 있어서 가장 효과적인 운전 조건임을 확인 할 수 있었다.

• IMMUNE NETWORK
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• 제5권4호
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• pp.199-204
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• 2005

Background: ADP-ribosyl pyrophosphatases (ADPRase) has been known to catalyze the hydrolysis of ADP-ribose to ribose-5-phosphate and AMP. The role of ADPRase has been suggested to sanitize the cell by removing potentially toxic ADP-ribose. In this study, we examined the effect of nitric oxide on ADPRase activity in macrophages. Methods: ADPRase activity was measured in NO-inducing J774 cells. For in vitro experiments, recombinant human ADPRase was prepared in bacteria. Results: ADPRase activity was increased by the treatment of exogenous NO generating reagent, sodium nitroprusside (SNP), in J774 cells. The increased ADPRase activity was mediated by the post-translational modification, likely to cause cADP-ribosylation via nitrosylation of cysteine residue on the enzyme. The stimulation with endogeneous NO inducers, $TNF-{\alpha}/IFN-{\gamma}$, also increased ADPRase activity through NO synthesis. Futhermore, ADPRase activity may be mediated by the post-translational modification of ADPRase, ADP-ribosylation. Conclusion: These results indicate that NO synthesized by macrophage activation plays a critical role in the increase in ADPRase activity following ADP-ribose metabolism.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.665-672
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• 2004

D-Ribose is a five-carbon sugar used for the commercial synthesis of riboflavin, antiviral agents, and flavor enhancers. Batch fermentations with transketolase-deficient B. subtilis JY1 were carried out to optimize the production of D-ribose from xylose. The best results for the fermentation were obtained with a temperature of $37^{\circ}C$ and an initial pH of 7.0. Among various sugars and sugar alcohols tested, glucose and sucrose were found to be the most effective for both cell growth and D-ribose production. The addition of 15 g/l xylose and 15 g/l glucose improved the fermentation performance, presumably due to the adequate supply of ATP in the xylose metabolism from D-xylulose to D-xylulose-5-phosphate. A batch culture in a 3.7-1 jar fermentor with 14.9 g/l xylose and 13.1 g/l glucose resulted in 10.1 g/l D-ribose concentration with a yield of 0.62 g D-ribose/g sugar consumed, and 0.25 g/l-h of productivity. Furthermore, the sugar utilization profile, indicating the simultaneous consumption of xylose and glucose, and respiratory parameters for the glucose and sucrose media suggested that the transketolase-deficient B. subtilis JY1 lost the glucose-specific enzyme II of the phosphoenolpyruvate transferase system.

• 한국식품영양과학회지
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• 제40권3호
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• pp.458-465
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• 2011

본 연구는 반응표면분석법(response surface methodology, RSM)을 적용하여 소맥 글루텐 산 가수분해물의 염 농도와 기질인 ribose와 소맥 글루텐 산 가수분해물의 농도가 MRP의 갈변도와 항산화 활성, 기호도에 미치는 영향을 분석하여 기질조건을 최적화 하고자 하였다. 종속변수 회귀식의 결정 계수($R^2$)는 각각 0.975, 0.960, 0.854로 나타났으며 반응모형에서 갈변도와 항산화 활성은 모두 ribose와 소맥 글루텐산 가수분해물의 농도가 증가함에 따라 증가하여 두 기질 농도에 영향을 크게 받았다. 그러나 갈변도는 소맥 글루텐산 가수분해물의 염 농도가 높아짐에 따라 감소하는 경향을 보였고 DPPH radical 소거활성은 염 농도의 영향을 받지 않았다. 관능적 기호도로 평가된 고기향미에 대한 향 특성은 소맥 글루텐의 염 농도가 낮으며 ribose의 농도에 비해 소맥글루텐 산 가수분해물의 농도가 높을 때 발현되었다. 본 실험에서 ribose와 소맥 글루텐 산 가수분해물을 이용한 MRP의 높은 DPPH radical 소거활성과 관능적으로 고기향미에 근접한 향 특성을 나타내는 최적조건으로서, 소맥 글루텐산 가수분해물의 염 농도가 3%, ribose 농도 6.2%와 소맥글루텐 산 가수분해물의 농도 13.27%로 나타났다. 이때 DPPH radical 소거활성은 700(DF)과 관능점수 8.42점을 만족시킬 수 있는 MRP를 얻을 수 있었다. 따라서 ribose와 소맥 글루텐 산 가수분해물로 제조된 MRP의 저염화에 따른 갈변도와 항산화 효과 및 관능적 기호도에 대하여 두 기질의 농도를 조절하여 최적화함으로써 이를 향상시킬 수 있을 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.560-564
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• 2013

${\small{D}}$-Ribose is a value-added five-carbon sugar used for riboflavin production. To investigate the effects of oxygen supply and mixed sugar concentration on microbial production of ${\small{D}}$-ribose, a transketolase-deficient Bacillus subtilis SPK1 was cultured batch-wise using xylose and glucose. A change of agitation speed from 300 rpm to 600 rpm at 1 vvm of air supply increased both the xylose consumption rate and ${\small{D}}$-ribose production rate. Because the sum of the specific consumption rates for xylose and glucose was similar at all agitation speeds, metabolic preferences between xylose and glucose might depend on oxygen supply. Although B. subtilis SPK1 can take up xylose and glucose by the active transport mechanism, a high initial concentration of xylose and glucose was not beneficial for high ${\small{D}}$-ribose production.

• 한국동물학회지
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• 제39권3호
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• pp.248-256
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• 1996

본 연구의 목적은 인간의 Poly(ADP-ribose) synthetase (PARS)의 cDNA를 클로닝하여 발현시키기 위해 수행하였다. 먼저, 인간의 PARS cDNA 전체를 포함한 pPARS3.1을 pGEM-7Zf(+) 등의 발현 벡터 클로닝하였다. 이 결과로 생성된 재조합 플라스미드 pPARS6.1이 인간의 PARS cDNA 전체를 포함하고, 올바른 방향으로 삽입되었는지를 확인하기 위해 제한효소 지도를 작성하였고, random primed DNA probe을 이용한 Southern blot 분석에 의해서 PARS가 클로닝되었다는 것을 확인하였다. 또한, 염기서열 분석 결과, 단백질 합성이 시작되는 유전 암호가 정확한 순서로 위치하고 있음을 확인하였다. 재조합된 pPARS6.1의 발현을 위해 배양시 0.4 mM IPTG로 처리하여 만들어진 인간의 PARS 단백질이 10% SDS-PAGE에서 120 kDa 위치에 이동하였다는 것을 nick-translated된 DNA를 probe로 이용하여 확인하였고, Southwestern blot 실험 결과 120 kDa과 98kDa에 위치하는 단백질이 DNA와 결합함을 알 수 있었다.

• 생명과학회지
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• 제20권12호
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• pp.1829-1837
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• 2010

Maillard 반응의 기본이 되는 당과 아미노산을 결정하기 위하여 미더덕 체액, 6탄당인 glucose와 5탄당인 ribose를 기본으로 하여, thiamine, 아미노산으로는 methionine, taurine, glycine, alanine, threonine, cystine 등을 사용하였고, 실용적인 방안을 강구하기 위하여 식용하는 양파, 마늘, 감귤, 생강, 녹차, 파 등을 첨가하여 반응액을 만들었다. Ribose와 thiamine 구가 패널로부터 가장 높은 평가를 받았고, 이어서 glucose와 thiamine 구가, ribose와 methionine 구는 아주 나쁜 풍미를 형성하는 것으로 나타났다. 함황화합물인 taurine을 첨가한 glucose 및 ribose 구는 보통의 평가로 나타났고, 체액에 당과 아미노산을 첨가한 경우도 thiamine 구만 보통으로 평가되었으나 나머지 아미노산의 경우 싫어 하는 것으로 나타났다. 기본의 액즙-당 반응구에서는 장쇄지방산의 함량이 31.32-62.71%로 가장 높은 함량을 보였고, A, B, C구에서는 1,2-bezenedicarboxylic acid dibutyl ester의 함량도 20%를 상회하는 것으로 나타나 이들 화합물이 전체 향기성분의 50% 이상으로 이들이 체액-당 반응계의 주된 화합물로 생성되어, 체액을 활용한 조미료의 생산을 저렴한 비용으로 할 수 있는 것으로 나타났다.

• Journal of Magnetics
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• 제19권1호
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• pp.20-27
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• 2014

This study examined the relationships between protein expression and Poly ADP ribose polymerase in brain cell death in brains damaged by thrombotic stroke and treated with the Full Wave- Cockroft Walton (FWCW) method of Transcranial Magnetic Stimulation (TMS). The two-way switching element for TMS drove a half-bridge inverter of the current resonance of direct current voltage (+) and direct current voltage (-), and the experiment was conducted by stimulating the mice with thrombotic stroke through a range of pulses. Thrombotic stroke was caused of ligation of the common carotid artery of male SD mice, and blood reperfusion was conducted five minutes later. Protein expression was examined in immune reaction cells, which reacted to an antibody to Poly ADP ribose polymerase in the cerebrum cells, and western blotting. Observations of the PARP changes after thrombotic stroke showed that the number of Poly ADP ribose polymerase reactions were significantly lower (p < 0.05) in the group treated with TMS of the FWCW than the group with thrombotic stroke 24 hours after its onset. The application of FWCW-TMS helped prevent the necrosis of nerve cells and might prevent the brain damage that occurs as a result of thrombotic stroke, and improve the function recovery and disorder of brain cells.