KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Determination of Adsorption Isotherms and Separation of L-arabinose and D-ribose in Cation Exchange Chromatography and HPLC

양이온 교환 크로마토그래피와 HPLC에서의 L-arabinose와 D-ribose의 분리 및 등온 흡착곡선 결정

  • Jeon, Young-Ju (Department of Chemical Engineering, Chungnam National University) ;
  • Kim, In-Ho (Department of Chemical Engineering, Chungnam National University)
  • Published : 2008.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

The use of L-carbohydrates and their corresponding nucleosides in medicinal application has greatly increased. For example L-ribose has been much in demand as the starting material for curing hepatitis B. High performance liquid chromatography (HPLC) method was studied for the analysis of ribose and arabinose fractions from ion exchange chromatography (IEC). Dowex Monosphere 99 Ca/320 resin was packed in IEC to separate ribose and arabinose under various operating conditions. $NH_{2}$ and sugar HPLC columns were then used to analyze the fractions from the IEC column. Pulse input method (PIM) was also used to measure adsorption isotherms of ribose and arabinose in the Dowex column and HPLC columns. Experimental results and simulations by ASPEN chromatography were compared with fair agreement.

Keywords

References

  1. De Muynck, C., Beauprez, J., Soetaert, W., and Erick, J. V. (2006), Boric Acid as a Mobile Phase Additive for High Performance Liquid Chromatography Separation of Ribose, Arabinose and Ribulose, J. Chromatogr A., 1101, 115-121 https://doi.org/10.1016/j.chroma.2005.09.068
  2. Zakaria, A., Tsuyoshi S., Shakhawat H., Masaru, V., Goro, T., and Ken I. (1999), Biochemical Preparation of L-Ribose and L-Arabinose from Ribitol : A New Approach, J. Biosci. Bioeng., 88(4), 444-448 https://doi.org/10.1016/S1389-1723(99)80225-4
  3. Stephen , J. A. (2005), L-ribose : An Easily Prepared Rare Sugar , Aust. J. Chem., 58, 58-59 https://doi.org/10.1071/CH04201
  4. http://www.dow.com/liquidseps/prod/chromato.htm
  5. Caruel, H., Rigal, H., and Gaset, A. (1991), Carbohydrate Separation by Ligand Exchange Liquid Chromatography, J. Chromatogr., 588, 84-104
  6. Kim, S. Y., Song, T., Lee, J. K., Suh, S. S., and Choi, M H. (2002), Adsorptive Correlations of Chromatography for Ibuprofen Enantiomers, HWAHAK KONGHAK, 40(3), 324-329
  7. Lee, E., Park, J. S., and Kim I. H. (2006), Determination of Loxoprofen Adsorption Isotherms by Frontal Analysis and Pulse Input Method, Korean J. Biotechnol. Bioeng., 21(5), 371-375
  8. Lambert A. T. V. and Jacques M. H. D. (1978), Ion Exchange Chromatography of Sugars, Sugar Alcohols, and Sugar Acids Using V.V. Spectrometry for Direct Detection, Carbohydr. Res., 62, 197-202 https://doi.org/10.1016/S0008-6215(00)80866-6
  9. Kim, J. I., Lee, C. H., and Koo, Y. M. (2005), Temperature Effret on the Retention Behavior of Sugar in Ion Exchange Chromatography, Korean Chem. Eng. Res., 43(6), 722-727