• 제목/요약/키워드: rhoA

검색결과 3,664건 처리시간 0.029초

Phospholipase D Is Not Involved in Rho A-Mediated Activation of Stress Fiber Formation

  • Leem, Sun-Hee;Shin, In-Cheol;Kweon, Soo-Mi;Kim, Seung-Il;Kim, Jae-Hong;Ha, Kwon-Su
    • BMB Reports
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    • 제30권5호
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    • pp.337-341
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    • 1997
  • In order to investigate the role of a small GTP-binding protein RhoA in lysophosphatidic acid (LPA)-induced stress fiber formation, C3 ADP-ribosyltransferase was prepared by expressing in E. coli and then applied to Rat-2 fibroblasts. C3 transferase isolated from E. coli was as effective as the toxin from Clostridium botulinum in ADP-ribosylation of RhoA. Incubation of the cells with C3 transferase for 2 days induced ADP-ribosylation of RhoA by a dose-dependent manner, with a sub-maximal induction at $25\;{\mu}g/ml$. As expected, LPA-induced stress fiber formation was completely blocked by pre-incubation with C3 transferase for 2 days. However, exogenously added C3 transferase had no significant effect on the formation of phosphatidylethanol by LPA. These results suggested that phospholipase D was not activated by RhoA in the LPA-induced stress fiber formation.

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A Small GTPase RHO2 Plays an Important Role in Pre-infection Development in the Rice Blast Pathogen Magnaporthe oryzae

  • Fu, Teng;Kim, Joon-Oh;Han, Joon-Hee;Gumilang, Adiyantara;Lee, Yong-Hwan;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • 제34권6호
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    • pp.470-479
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    • 2018
  • The rice blast pathogen Magnaporthe oryzae is a global threat to rice production. Here we characterized RHO2 gene (MGG_02457) that belongs to the Rho GTPase family, using a deletion mutant. This mutant ${\Delta}Morho2$ exhibited no defects in conidiation and germination but developed only 6% of appressoria in response to a hydrophobic surface when compared to the wild-type progenitor. This result indicates that MoRHO2 plays a role in appressorium development. Furthermore, exogenous cAMP treatment on the mutant led to appressoria that exhibited abnormal morphology on both hydrophobic and hydrophilic surfaces. These outcomes suggested the involvement of MoRHO2 in cAMP-mediated appressorium development. ${\Delta}Morho2$ mutation also delayed the development of appressorium-like structures (ALS) at hyphal tips on hydrophobic surface, which were also abnormally shaped. These results suggested that MoRHO2 is involved in morphological development of appressoria and ALS from conidia and hyphae, respectively. As expected, ${\Delta}Morho2$ mutant was defective in plant penetration, but was still able to cause lesions, albeit at a reduced rate on wounded plants. These results implied that MoRHO2 plays a role in M. oryzae virulence as well.

HOMOTOPY FIXED POINT SET $FOR \rho-COMPACT$ TORAL GROUP

  • Lee, Hyang-Sook
    • 대한수학회보
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    • 제38권1호
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    • pp.143-148
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    • 2001
  • First, we show the finiteness property of the homotopy fixed point set of p-discrete toral group. Let $G_\infty$ be a p-discrete toral group and X be a finite complex with an action of $G_\infty such that X^K$ is nilpotent for each finit p-subgroup K of $G_\infty$. Assume X is $F_\rho-complete$. Then X(sup)hG$\infty$ is F(sub)p-finite. Using this result, we give the condition so that X$^{hG}$ is $F_\rho-finite for \rho-compact$ toral group G.

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SHARP FUNCTION AND WEIGHTED $L^p$ ESTIMATE FOR PSEUDO DIFFERENTIAL OPERATORS WITH REDUCED SYMBOLS

  • Kim, H.S.;Shin, S.S.
    • East Asian mathematical journal
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    • 제6권2호
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    • pp.133-144
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    • 1990
  • In 1982, N. Miller [5] showed a weighted $L^p$ boundedness theorem for pseudo differential operators with symbols $S^0_{1.0}$. In this paper, we shall prove the pointwise estimates, in terms of the Fefferman, Stein sharp function and Hardy Littlewood maximal function, for pseudo differential operators with reduced symbols and show a weighted $L^p$-boundedness for pseudo differential operators with symbol in $S^m_{\rho,\delta}$, 0{$\leq}{\delta}{\leq}{\rho}{\leq}1$, ${\delta}{\neq}1$, ${\rho}{\neq}0$ and $m=(n+1)(\rho-1)$.

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Effect of the Injection Parameters on Diesel Spray Characteristics

  • Song Kyu Keun;Sim Sang Cherl;Jung Byong Koog;Kim Hyung Gon;Kim Jang Heon
    • Journal of Mechanical Science and Technology
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    • 제19권6호
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    • pp.1321-1328
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    • 2005
  • The characteristics of the diesel spray have affected certain aspects of engine performance, such as the power, fuel consumption, and emissions. Therefore, this study was performed to investigate the effects of various injection parameters. In order to obtain the effect of injection parameters on diesel spray characteristics, the experiment is performed by using a high temperature and pressure chamber. The behaviors of the spray are visualized by using a high speed video camera, spray angle, penetration, and various other things. The results of the experiment are summarized as follows. (1) The correlation of the spray penetration can be expressed as follows. $$0< t $$t_{b} (2) The correlation of the spray angle can be expressed as follows $$T_a=293K\;tan({\theta}/2)=0.59({\rho}a/{\rho}f)^{0.437}$$ $$T_a=473K\;tan({\theta}/2)=0.588({\rho}a/{\rho}f)^{0.404}$$ (3) The measured macro characteristics that include the spray tip penetration and spray angle corresponded with the established correlations.

ON A FUZZY BANACH SPACE

  • Rhie, G.S.;Hwang, I.A.
    • 충청수학회지
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    • 제13권1호
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    • pp.71-78
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    • 2000
  • The main goal of this paper is to prove the following theorem ; Let (X, ${\rho}_1$) be a fuzzy normed linear space over K and (Y, ${\rho}_2$) be a fuzzy Banach space over K. If ${\chi}_{B_{{\parallel}{\cdot}{\parallel}}}{\supseteq}{\rho}*$, then (CF(X,Y), ${\rho}*$) is a fuzzy Banach space, where ${\rho}*(f)={\vee}{\lbrace}{\theta}{\wedge}\frac{1}{t({\theta},f)}\;{\mid}\;{\theta}{\in}(0,1){\rbrace}$, $f{\in}CF(X,Y)$, $B_{{\parallel}{\cdot}{\parallel}}$ is the closed unit ball on (CF(X, Y), ${\parallel}{\cdot}{\parallel}$ and ${\parallel}f{\parallel}={\vee}{\lbrace}P^2_{{\alpha}^-}(f(x))\;{\mid}\;P^1_{{\alpha}^-}(x)=1,\;x{\in}X{\rbrace}$, $f{\in}CF(X,Y)$, ${\alpha}{\in}(0,1)$.

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FOLIATIONS ASSOCIATED WITH PFAFFIAN SYSTEMS

  • Han, Chong-Kyu
    • 대한수학회보
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    • 제46권5호
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    • pp.931-940
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    • 2009
  • Given a system of smooth 1-forms $\theta$ = ($\theta^1$,...,$\theta^s$) on a smooth manifold $M^m$, we give a necessary and sufficient condition for M to be foliated by integral manifolds of dimension n, n $\leq$ p := m - s, and construct an integrable supersystem ($\theta,\eta$) by finding additional 1-forms $\eta$ = ($\eta^1$,...,$\eta^{p-n}$). We also give a necessary and sufficient condition for M to be foliated by reduced submanifolds of dimension n, n $\geq$ p, and construct an integrable subsystem ($d\rho^1$,...,$d\rho^{m-n}$) by finding a system of first integrals $\rho=(\rho^1$,...,$\rho^{m-n})$. The special case n = p is the Frobenius theorem on involutivity.

Hall effect of K-doped $BaFe_2As_2$ superconducting thin films

  • Son, Eunseon;Lee, Nam Hoon;Hwang, Tae-Jong;Kim, Dong Ho;Kang, Won Nam
    • 한국초전도ㆍ저온공학회논문지
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    • 제15권3호
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    • pp.5-8
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    • 2013
  • We have studied Hall effect for potassium (K)-doped $BaFe_2As_2$ superconducting thin films by analyzing the relation between the longitudinal resistivity (${\rho}_{xx}$) and the Hall resistivity (${\rho}_{xy}$). The thin films used in this study were fabricated on $Al_2O_3$ (000l) substrates by using an ex-situ pulsed laser deposition (PLD) technique under a high-vacuum condition of ~$10^{-6}$ Torr. The samples showed the high superconducting transition temperatures ($T_c$) of ~ 40 K. The ${\rho}_{xx}$ and the ${\rho}_{xy}$ for K-doped $BaFe_2As_2$ thin films were measured by using a physical property measurement system (PPMS) with a temperature sweep (T-sweep) mode at an applied current density of $100A/cm^2$ and at magnetic fields from 0 up to 9 T. We report the T-sweep results of the ${\rho}_{xx}$and the ${\rho}_{xy}$ to investigate Hall scaling behavior on the basis of the relation of ${\rho}_{xy}={A{\rho}_{xx}}^{\beta}$. The ${\beta}$ values are $3.0{\pm}0.2$ in the c-axis-oriented K-doped $BaFe_2As_2$ thin films, whereas the thin films with various oriented-directions like a polycrystal showed slightly lower ${\beta}$ than that of c-axis-oriented thin films. Interestingly, the ${\beta}$ value is decreased with increasing magnetic fields.

대식세포의 혈청으로 식균된 자이모잔의 탐식능에 대한 삼잎국화 추출물의 효과 (Effects of Rudbeckia laciniata Extract on Phagocytosis of Serum-Opsonized Zymosan Particles in Macrophages)

  • 김준섭
    • 한국식품영양학회지
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    • 제29권3호
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    • pp.341-346
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    • 2016
  • Phagocytosis is a primary and an essential step of host defense, and is triggered by the interaction of particles with specific receptor of macrophages. In this study, we investigated the effect of extracts of Rudbeckia laciniata (RLE) on the phagocytic activity of macrophage, by monitoring the phagocytosis-associated signal transduction. RLE markedly increased phagocytosis of serum-opsonized zymosan particles (SOZ), while phagocytosis of IgG-opsonized zymosan particles (IOZ) or none-opsonized zymosan particles (NOZ) remained unaffected. However, RLE did not affect the binding of opsonized zymosan particles (OZ) with the cell surface of macrophage. This suggests that RLE may regulate SOZ-induced intracellular signaling during phagocytosis of macrophage. To confirm this hypothesis, we investigated whether RLE was involved in the RhoA-mediated signal transduction during phagocytosis of SOZ. Inhibitors of the RhoA-mediated signaling pathway, such as Y-27632 (for ROCK), ML-7 (for MLCK), and Tat-C3 (for RhoA), totally blocked phagocytosis of SOZ enhanced by RLE, as well as phagocytosis of SOZ. Additionally, RhoA activity was markedly increased when cells were treated with RLE, suggesting that RLE could increase the phagocytic activity of macrophage via RhoA-ROCK/MLCK signal pathway. Thus, RLE may be used to develop functional foods for immunity.

디젤분무 특성에 미치는 분사인자의 영향 (Effect of Injection Parameters on Diesel Spray Characteristics)

  • 심상철;정병국;안병규;김장헌;정재연;송규근
    • Journal of Advanced Marine Engineering and Technology
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    • 제29권1호
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    • pp.17-24
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    • 2005
  • The characteristics of diesel spray have effect on the engine Performance such as power. fuel consumption and emissions. Therefore, This study was Performed to investigate the effect of various injection parameters. In this study. the experiment is performed by using the high temperature and high pressure chamber. Spray behaviors are visualized by using the high speed camera and spray angle. Penetration etc. are measured. Experimental results are summarized as follows ; 1) Correlations of spray Penetration is expressed as follows $$0 $$t_b 2) Correlations of spray Angle is expressed as follows $$T_a=293K \;;\; tan({\theta}/2)=0.59({\rho}_a/{\rho}_f)^{0.437}$$ $$T_a=473K\;;\; tan({\theta}/2)=0.588({\rho}_a/{\rho}_f)^{0.404}$$ 3) The measured macro characteristics - spray tip penetration and spray angle agreed well with established correlations.