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http://dx.doi.org/10.5423/PPJ.OA.04.2018.0069

A Small GTPase RHO2 Plays an Important Role in Pre-infection Development in the Rice Blast Pathogen Magnaporthe oryzae  

Fu, Teng (Division of Bioresource Sciences, and Bioherb Research Institute, Kangwon National University)
Kim, Joon-Oh (Division of Bioresource Sciences, and Bioherb Research Institute, Kangwon National University)
Han, Joon-Hee (Division of Bioresource Sciences, and Bioherb Research Institute, Kangwon National University)
Gumilang, Adiyantara (Division of Bioresource Sciences, and Bioherb Research Institute, Kangwon National University)
Lee, Yong-Hwan (Department of Agricultural Biotechnology, and Center for Fungal Genetic Resources, Seoul National University)
Kim, Kyoung Su (Division of Bioresource Sciences, and Bioherb Research Institute, Kangwon National University)
Publication Information
The Plant Pathology Journal / v.34, no.6, 2018 , pp. 470-479 More about this Journal
Abstract
The rice blast pathogen Magnaporthe oryzae is a global threat to rice production. Here we characterized RHO2 gene (MGG_02457) that belongs to the Rho GTPase family, using a deletion mutant. This mutant ${\Delta}Morho2$ exhibited no defects in conidiation and germination but developed only 6% of appressoria in response to a hydrophobic surface when compared to the wild-type progenitor. This result indicates that MoRHO2 plays a role in appressorium development. Furthermore, exogenous cAMP treatment on the mutant led to appressoria that exhibited abnormal morphology on both hydrophobic and hydrophilic surfaces. These outcomes suggested the involvement of MoRHO2 in cAMP-mediated appressorium development. ${\Delta}Morho2$ mutation also delayed the development of appressorium-like structures (ALS) at hyphal tips on hydrophobic surface, which were also abnormally shaped. These results suggested that MoRHO2 is involved in morphological development of appressoria and ALS from conidia and hyphae, respectively. As expected, ${\Delta}Morho2$ mutant was defective in plant penetration, but was still able to cause lesions, albeit at a reduced rate on wounded plants. These results implied that MoRHO2 plays a role in M. oryzae virulence as well.
Keywords
appressorium formation; Magnaporthe oryzae; pathogenicity; Rho GTPase;
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