• 한국가축번식학회지
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• 제27권4호
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• pp.275-280
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• 2003

This study was carried out to investigate the effect of cysteamine addition during in vitro maturation, fertilization and culture of porcine oocytes. Oocytes were matured for the first 22 h in mTCM -199 media supplemented with or without 150 $\mu$M cysteamine. They then were matured for an additional 22 h in mTCM-199 media without hormones supplemented with or without 150 $\mu$M cysteamine. When cumulus-oocyte complexes (COCs) were matured in the mTCM-199 media supplemented with cysteamine, the rates of GVBD and maturation (metaphase II) were enhanced as compared to the media without the addition of cysteamine. Also, when COCs were matured in the mTCM-199 media supplemented with cysteamine, the rates of sperm penetration, male pronucleus formation, cleavage and blastocyst formation after in vitro fertilization were enhanced as compared to the media without the addition of cysteamine. In conclusion, it was suggested that oocytes matured for the first 22 h in mTCM-199 media supplemented with 150 $\mu$M cysteamine increased the rates of metaphase II, sperm penetration, male pronucleus and blastocyst formation were higher as compared to the media without addition of cysteamine.

• 복식
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• 제51권6호
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• pp.5-24
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• 2001

This paper is intended to explore femininity the ideal beauty of body and the features in fashion pursued in the Fashion system and the Anti-Fashion Movement in the Victorian period, on which the modern fashion is based. For the informative facts needed in this paper, books on history, fashion history, feminism, art history of aestheticism and the ideal beauty of body are referred to. On the part Ⅱ of this paper, the femininity and the ideal beauty of body implied in the Aesthetic Movement and Rational Dress Movement as the anti-fashion movement in the Victorian period will be reviewed. Following are the conclusion : First, the works in Aesthetic Movement mainly include the image of sensual female. The essence of femininity is categorized by cultural value, poetic spirit. appetite for sex and self-expression. The ideal beauty of body pursued in this movement is the beauty of immatured body, which means rejection of maternity as well as appetite for sex in the form of metaphor of the power and enthusiasm of female. The features of Aesthetic Movement emphasize the image of sensual and characteristic woman. These features are expressed in the natural waist line and the vertical H silhouette of high waist, natural exposure of body by means of drapery, simplicity and decency by design without fixed forms and seemingly faded colors. Second, Rational Dress Movement attempted to evaluate the femininity in a different way and ultimately pursued masculinity. Therefore, morality, liberty, intelligence, spirituality, self-control, willingness, which had been believed to exclusively belong to male, are added to the categories of femininity. The ideal beauty of body is expressed in the form of Venus Coelestic which is refined and strong. This symbolizes woman's freeing from the fate of reproduction and subordinate relationship with male, morality, decision of one's own and willingness. The features of Rational Dress Movement represent the image of strong-willed and moral woman in its internal meaning. The features of its fashions represent the concealment of the body, emphasis on activeness in pants without decoration and simplicity in its external form. All these features resulted from the pursuit of masculinity.

• 복식
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• 제51권2호
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• pp.169-180
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• 2001

This paper is intended to explore femininity, the ideal beauty of body and the features in fashion pursued in the fashion system and the Anti-Fashion Movement in the Victorian period, on which the modern fashion is based. For the informative facts needed in this paper, books on history, fashion history, feminism, art history of aestheticism and tole ideal beauty of body are referred to. On the part I of this paper, the femininity and the ideal beauty of body implied in the fashion system in the Victorian period will be reviewed. following are the conclusion : First, in the Victorian period, the value of femininity is put on the body of female by the discrimination of sex divided in two. Consequently, the characteristics of femininity mainly include dependence, passiveness, emotion, beauty, maternity, innocence, and purity. To emphasize the function of reproduction as primary duty and nature of female, the ideal beauty of body is represented in the form of Venus Naturalis, which symbolizes the fertility. And the external form of this body is expressed in slum waist line, ample busom and hip in fashion. Second, the features of this fashion are classified into three categories by their internal value : images of subordinate female, sensual female and maternal female 1) The image of subordinate female is expressed by concealment of legs, tightening the upper part of the body in corset and restriction on action by the crinoline 2) The image of sensual female is revealed in brazing colors and decoration, excessive exposure of the upper part of the body and hip by means of bustle. 3) The image of maternity is expressed in swollen skirt of crinoline, oval bustle silhouette metaphoring the extended womb resulted from pregnancy.

• 한국가축번식학회지
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• 제26권4호
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• pp.347-351
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• 2002

Transgenes in HSY-TK gene driven by the lck promoter was tested for the expression in immune cells (Jurkat cells) to apply xenotransplantation of human cells into transgenic animals for the potential use of the proliferation or differentiation of human stem cells in the large animal such as an pig. Also, lck-CFP gene was used for transfection experiment into Jurkat cell to confirm the proper regulation of lck promoter for transgene expression in the T cells. Transfection of lck-GFP gene into Jurkat ceils induced CFP expression in transfected cells. The expression of Ick-TK and Ick-CFP genes was confirmed by RT-PCR using RNAs extracted from Jurkat cells, When Jurkat cells transfected with TK and CFP genes were selected against G418 or gancyclovir treatments, Jurkat cells transfected with TK gene were not proliferated in G4i8 and gancyclovir medium while intact cells or cells transfected with CFP gene could grow in gancyclovir medium. However, Jurkat cells transfected with TK or GFP gene were proliferated in G418 medium probably due to Neo$^{r}$ gene in the vector. Gancyclovir treatment destroyed Jurkat cells expressing TK gene indicating that T-cells expressing TK gene can be selectively eliminated by TK gene expression driven by lck promoter.

• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2008년도 학술대회 2부
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• pp.470-474
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• 2008

미디어 이미지는 변화를 계속하고 있다. 그에 따라 이미지는 다양한 분야에서 다른 방식으로 만들어지고, 재생산되고 있다. 이미지의 생산과 소비에 관한 패러다임은 점점 가속화되고 순환하고 있다. 이미지의 생산과 소비에 관한 관계 또한 변화하고 있으며, 생산자와 소비자의 경계 또한 모호해져 가고 있다. 미디어 이미지가 보여주는 다른 이미지의 차용이나 변형은 오랫동안 커뮤니케이션의 주요 수단으로 사용되었던 문자나 부호, 기호마저도 본연의 의미를 상실하고, 새로운 이미지 표현 수단 및 미학적 표현으로 사용되고 있다. 따라서 본 논문에서는 기호의 하나인 등고선을 이용하여 새로운 미학적 표현 수단으로 발전시키고자 하였다. 또한 그 방법에 있어서, 이미지의 생성에 소비자를 적극적으로 개입시켜 이미지 소비자와 생산자와의 관계를 변화시켜, 일방적인 소비와 생성의 관계가 아닌 소비자가 이미지를 생성시키는 주체로 작용시키고자 하였다. 즉, 기능적인 역할의 등고선을 본연의 의미를 상실시켜 새로운 미학적 표현의 방법으로 등고선을 이용한 라인드로잉의 이미지를 생성하고, 시간과 관객의 행동에 따라 끊임없이 재생성 되어 관객이 이미지의 생성에 직접적으로 작용하고자 한다. 이것은 기호를 커뮤니케이션을 위한 약속으로 바라보는 고정적 시각에서 벗어나 미학적 표현 가능성을 보여주고, 지형에 대한 새로운 인식을 환기시키고자 한다.

• 한국가축번식학회지
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• 제26권4호
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• pp.385-394
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• 2002

This study was constructed the correlations of the embryonic developmental rates and the frequency of chromosome aberration using ear-skin-fibroblast cell in nuclear transfer (NT) derived embryos. Karyoplast-oocyte complexes were fused and activated simultaneously, then cultured for seven days to assess development. The developmental rates of NT and in vitro fertilization (IVF) embryos were 55.4% vs 63.5%, 31.7% vs 33% and 13.4% vs 16.8% in 2 cell, 8 cell and blastocyst, respectively. Firstly, the frequency of chromosome aberrations were evaluated using fluorescent in situ hybridization (FISH) technique with porcine chromosome 1 submetacentric specific probe. Chromosome aberration was detected at day 3 on the embryo culture, the percentages of chromosomal aneuploidy in NT and IVF embryos at 4-cell stage were 40%, 31.3%, respectively. Secondly, embryonic fragmentation was evaluated at 4-cell stage embryo. Frequency of embryonic fragmentations was in 51.3% of NT, 61.3% of IVF, 28.9% of parthenogenetic activation at 4-cell stage. The proportion of fragmentation in NT embryos was higher than activation embryos. This result indicates that chromosomal abnormalities and embryonic fragments are associated with low developmental rate in porcine NT embryo. It is also suggest that abnormal porcine embryos produced by NT related with lower implantation rate, increased abortion rate and production of abnormal fetuses.

• 세라미스트
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• 제21권1호
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• pp.55-63
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• 2018

Recently, display technology has been focused in regard with with color reproduction, contrast ratio, image resolution and color bit. Among these technologies, the color reproducibliity of White, Red, Green, and Blue is associated with the TV plaform and is expressed as a major technology. Major TV platforms are divided into three categories since 2015, including LCD-based phosphor coverted LED BLU technology, QD sheet technology using nano-sized quantum dots, and OLED technology. In this paper, we describe the color reproducibility definition and background, luminescent materials with wide color gamut, color reproducibility of TV display performance, and discuss about next luminescent materials.

• 한국학교수학회논문집
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• 제19권1호
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• pp.1-19
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• 2016

본 연구는 오늘날 사용되는 식의 표기를 괄호 생략의 관점에서 고찰하였다. 먼저 연산 기호 생략 규칙과 관련한 초 중등 교과 내용을 검토해본 결과, '$48{\div}2(9+3)$의 계산 결과는 얼마인가?'라는 물음에 답할 명시적 근거를 찾기 어려웠다. 다음 표기에 관한 근본적인 탐구를 위해 전위 표기법, 중위 표기법, 후위 표기법의 특징을 살펴보고, 중위 표기법이 갖는 장 단점을 확인하였다. 동시에 표기법의 발전을 괄호 생략의 관점에서 조명해 보았다. 이러한 고찰로부터 연산 생략은 곧 함수의 이미지라는 관점이 괄호 생략을 원활하게 해석할 수 있는한 방법임을 확인할 수 있었다. 이를 통해 역사 발생적 원리에 따른 수학적 표기 지도, 중위 표기법의 선택 맥락 이해와 난점 극복을 위한 노력의 재현, 연산자를 포함한 생략된 괄호를 찾아 근본 식을 파악하는 활동, 곱셈이 생략된 경우를 함수의 이미지로서 간주하는 관점의 명시 등을 포함한 몇 가지 지도방안을 제안하였다.

• 펄프종이기술
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• 제39권2호
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• pp.31-37
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• 2007

Lithography like off-set printing is processed using the repellent properties between water and oil, so all inks for off-printing must work with dampening solution(water). The dampening water may cause the emulsification of ink by the printing pressure in the printing nip. This study aimed to investigate the effect of emulsified inks on print mottle. The cyan ink was emulsified artificially with the different IPA(isopropyl alcohol) content in this study. We evaluated the print mottle by densitometer and image analysis method. The print mottle phenomenon was directly affected by IPA content. The emulsification of inks also had an influence on flow properties of inks and it increased ink transfer rate. It, however, caused low ink density. Moreover the emulsified inks were spreaded to around dots and cause the thinning density on the non-printing area like print mottle. The trial printing showed that the emulsified inks also cause scumming on the printing result with little mistake of adjusting dampening solution and mostly decreasing dot reproduction. We could, therefore, find out the obvious effect of emulsified inks on print mottle.

• Reproductive and Developmental Biology
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• 제37권4호
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• pp.247-253
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• 2013

Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young piglet separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was carried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age dependent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.