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Effect of Cysteamine on In Vitro Maturation, Fertilization and Culture of Porcine Oocytes  

Yi, Y. J (Division of Animal Science ＆ Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Kim, M. Y. (Division of Animal Science ＆ Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Lee, S. H. (College of Visual Image ＆ Health, Kongju National University)
T. S. Min (Department of Life Science, Korea Science and Engineering Foundation)
D. I. Jin (Division of Animal Science ＆ Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Park, C. S. (Division of Animal Science ＆ Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.4, 2003 , pp. 275-280 More about this Journal
Abstract
This study was carried out to investigate the effect of cysteamine addition during in vitro maturation, fertilization and culture of porcine oocytes. Oocytes were matured for the first 22 h in mTCM -199 media supplemented with or without 150 $\mu$M cysteamine. They then were matured for an additional 22 h in mTCM-199 media without hormones supplemented with or without 150 $\mu$M cysteamine. When cumulus-oocyte complexes (COCs) were matured in the mTCM-199 media supplemented with cysteamine, the rates of GVBD and maturation (metaphase II) were enhanced as compared to the media without the addition of cysteamine. Also, when COCs were matured in the mTCM-199 media supplemented with cysteamine, the rates of sperm penetration, male pronucleus formation, cleavage and blastocyst formation after in vitro fertilization were enhanced as compared to the media without the addition of cysteamine. In conclusion, it was suggested that oocytes matured for the first 22 h in mTCM-199 media supplemented with 150 $\mu$M cysteamine increased the rates of metaphase II, sperm penetration, male pronucleus and blastocyst formation were higher as compared to the media without addition of cysteamine.
Keywords
Maturation; Cysteamine; Male pronucleus; Blastocyst;
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