[KSCI] Korea Science Citation Index Service

Chromosome Aberrations in Porcine Embryo Produced by Nuclear Transfer with Somatic Cell

K. S. Chung (Animal Resource Research Center, Konkuk University)
Ko, S. A (Animal Resource Research Center, Konkuk University)
S. J. Song (Animal Resource Research Center, Konkuk University SamSung Cheil Hospital, lab of Reproductive Biology and infertility)
J. T. Do (College of Visual Image & Health, Kongju National University & Research Center for Transgenic Cloned Pigs.)
Park, Y. S. (SamSung Cheil Hospital, lab of Reproductive Biology and infertility)
Lee, H. T. (Department of National Science & technology, Seoul National University)

Publication Information

Korean Journal of Animal Reproduction / v.26, no.4, 2002 , pp. 385-394 More about this Journal

Abstract

This study was constructed the correlations of the embryonic developmental rates and the frequency of chromosome aberration using ear-skin-fibroblast cell in nuclear transfer (NT) derived embryos. Karyoplast-oocyte complexes were fused and activated simultaneously, then cultured for seven days to assess development. The developmental rates of NT and in vitro fertilization (IVF) embryos were 55.4% vs 63.5%, 31.7% vs 33% and 13.4% vs 16.8% in 2 cell, 8 cell and blastocyst, respectively. Firstly, the frequency of chromosome aberrations were evaluated using fluorescent in situ hybridization (FISH) technique with porcine chromosome 1 submetacentric specific probe. Chromosome aberration was detected at day 3 on the embryo culture, the percentages of chromosomal aneuploidy in NT and IVF embryos at 4-cell stage were 40%, 31.3%, respectively. Secondly, embryonic fragmentation was evaluated at 4-cell stage embryo. Frequency of embryonic fragmentations was in 51.3% of NT, 61.3% of IVF, 28.9% of parthenogenetic activation at 4-cell stage. The proportion of fragmentation in NT embryos was higher than activation embryos. This result indicates that chromosomal abnormalities and embryonic fragments are associated with low developmental rate in porcine NT embryo. It is also suggest that abnormal porcine embryos produced by NT related with lower implantation rate, increased abortion rate and production of abnormal fetuses.

Keywords

Porcine; Low development; Chromosome abnormality; Fragmentation;

Citations & Related Records

Reference

1	Campbell, K. H. S., McWhir, J., Ritchie, W. A. and Wilmut, J. 1996. Sheep cloned by nuclear transfer from a cultured cell line. Nature, 380: 64-66
2	Cibelli, J. B., Stice, S. L., Gollueke, P. J., Kane, J. J., Jerry, J., Blackwell, C., Ponce de Leon, A. and Robl, J. M. 1998. Cloned transgenic calves produced from non-quiescent fetal fibroblasts. Science, 280: 1256-1258 DOI PUBMED ScienceOn
3	Dasso, M. and Newport, J. W. 1990. Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus. Cell, 61:811-823 DOI ScienceOn
4	Kawarasakki, T., Sone, M., Yoshida, M. and Bamba, K. 1996. Rapid and simultaneous detection of chromosome Y-and I-bearing porcine spermatozoa by fluorescence in situ hybridization. Mol. Reprod. Dev., 43:548-553 DOI ScienceOn
5	King, W. A. 1990. Chromosomal abnormalities and pregnancy failure in domastic animals. In: McFeely RA(ed.), Advances in Veterinary Science and Comparative Medicine. San Diego, CA: Academic Press, 34:229-250
6	Tao, T., Machaty, Z., Boquest, A. C., Day, B. N. and Prather, R. S. 1999. Development of pig embryos reconstructed by micro injection of culture fetal fibroblast cells into in vitro matured oocytes. Anim. Reprod. Sci., 56: 133-141 DOI ScienceOn
7	Viuff, D., Greve, T., Avery, B., Hyttel, P., Brockhoff, P. B. and Thomsen, P. D. 2000. Chromosome aberrations in in vitro-produced bovine embryos at days 2 - 5 post-insemination. BioI. Reprod., 63:1143-1148
8	Sullivan, W., Daily, D. R., Fogarty, P., Yook, K. J. and Pimpinelli, S. 1993. Delays in anaphase initiation occur in individual nuclei of the syncytial Drosophila embryo. Mol. Biol. Cell., 4:885-896 DOI PUBMED
9	Hassanane, M., Kovacs, A., Laurent, P., Lindblad, K. and Gustavsson, I. 1999. Stimutaneous detection of X-and Y-bearing bull spermatozoa by double colour fluorescence hybridization. Mol. Reprod. Dev., 53:407-412 DOI ScienceOn
10	Wang, W. H., Abeydeera, L. R., Han, Y. M., Prather, R. S. and Day, B. N. 1999. Morphologic evaluation and actin filament distribution in porcine embryos produced in vitro and in vivo. BioI. Reprod., 60:1020-1028 DOI ScienceOn
11	Verma, P. J., Du, Z. T., Crocker, L., Faast, R., Grupen, C. G., Mcllfatrick, S. M., Ashman, R. J., Lyons, I. G. and Nottle, M. B. 2000. In vitro development of porcine nuclear transfer embryos constructed using fetal fibroblasts. Mol. Reprod. Dev., 57:262-269 DOI ScienceOn
12	Wells, D. N., Misica, P. M., Tervit, H. R. and Vivanco, W. H. 1998. Adult somatic cell nuclear transfer in used to preserve the last surviving cow of the Enderby Island cattle breed. Reprod. Ferti!. Dev., 10:369-378 DOI ScienceOn
13	Magnuson, T., Debrot, S. and Dimpfl, J. 1985. The early lethality of autosomal monosomy in mouse. J. Exp. Zool., 236:353-360 DOI ScienceOn
14	Rosenkrans, C. F., Zeng, J. R., G. Q., McNamara, G. T., Schoff, P. K. and First, N. L. 1993. Development of bovine embryos in vitro as affected by energy substrates. BioI. Reprod., 49:459-462 DOI ScienceOn
15	Wilmut, I., Schnieke, A. E., McWhir, J, Kind, A. J. and Campbell, K. H. S. 1997. Viable off-spring derived from fetal and adult mammalian cells. Nature, 385:810-813 DOI ScienceOn
16	Kawahara, M., Mori, T., Tanaka, H. and Shimizu, H. 2002. The suppression of fragmentation by stabilization of actin filament in porcine enucleated oocytes. Theriogenology, 58:1081-1095
17	Wells, D. N., Misica, P. M., Day, T. A. and Tervit, H. R. 1997. Production of cloned lambs from an established embryonic cell line: a comparison between in vivo and in vitro matured cytoplasts. BioI. Reprod., 57:385-393 DOI ScienceOn
18	Wakayama, T., Perry, A. C. F., Zuccotti, M., Johnson, K. R. and Yanagimachi, R. 1998. Full -term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394:369-374 DOI ScienceOn
19	Telford, N. A., Watson, A. and Schultz, G. A. 1990. Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Mol. Reprod. Dev., 26:90-100 DOI ScienceOn
20	Jantsch, M., Hamilton, B., Mayr, B. and Schweizer, D. 1990. Meiotic chromosome behaviour reflects levels of sequence divergence in Sus scrofa domestica satellite DNA Chromosome, 99:330-335
21	Betthauser, J., Forsberg, E., Augenstein, M., Childs, L., Eilertsen, K., Enos, J., Forsythe, T., Golueke, P., Jurgella, G., Koppang, R., Lesmeister, T., Mallon, K., Mell, G., Misica, P., Pace, M., Pfister-Genskow, M., Strelchenko, N., Voelker, G., Watt, S., Thompson, S. and Bishop, M. 2000. Production of cloned pigs from in vitro systems. Nat. Biotech., 18:1055-1059
22	Cassar, G., King, W. A. and King, G. J. 1994. Influence of sex on early growth of pig conceptuses. J. Reprod. Fertil., 101:317-320 DOI PUBMED ScienceOn
23	Cheong, H. T., Ikesa, K., Martinez, M. A., Kataguri, S. and Takahashi, Y. 2000. Development of reconstituted pig embryos by nuclear transfer of cultured cumulus cells. Reprod. Fertil. Dev., 12:15-20
24	Handyside, A. H. and Delhanty, J. D. A. 1997. Detection of chromosomal abnormalities in human preimplantation embryos using FISH. Assist. Reprod. Genet., 13:137-139
25	Parrish, J. J., Susko-Parrish, J., Winer, M. A. and First, N. L. 1988. Capacitation of bovine sperm by heparin. BioI. Reprod., 38:1171-1180 DOI ScienceOn
26	Pergament, E., Fiddler, M., Cho, N., Johnson, D. and Holmgren, W. J. 1994. Sexual differentiation and preimplantation cell growth. Hum. Reprod., 9:1730-1732 DOI PUBMED
27	Kawarasaki, T., Matsumoto, K., Chikyu, M., Itagati, Y. and Horiuchi, A. 2000. Sexing of porcine embryo by in situ hybridization using chromosome Y-and I-specific DNA probes. Theriogenology, 53:1501-1509
28	Kuhholzer, B. and Prather, R. S. 2000. Advances of livestock nuclear transfer. Proc. Soc. Exp. BioI. Med., 224:240-245 DOI ScienceOn
29	Polejaeva, I. A., Chen, S. H., Vaught, T. D., Page, R. L., Mullins, J., Ball, S., Dai, Y. F., Boone, J., Walker, S., Ayares, D. L., Colman, A. and Campbell, K. H. S. 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature, 407:86-90
30	Burgoyne, P. S., Holland, K. and Strephens, R. 1991. Incidence of numerical chromosome abnormalities in human pregnancy estimated from induced and spontaneous abortion data. Hum. Reprod., 6:555-565 DOI PUBMED
31	Onishi, A., Iwamoto, M., Akita, T., Mikawa, S., Takeda, K., Awata, T., Hanada, H. and Perry, A. C. F. 2000. Pig cloning by microinjection of fetal fibroblast nuclei. Science, 289:1188-1190
32	Kopscny, V. 1989. High resolution autoradiographic studies of comparative nuc1eogenesis and genome activation during early embryo genesis in pig, man and cattle. Reprod. Nutr. Dev., 29:589-600 DOI PUBMED
33	McGrath, J. and Solter, D. 1986. Nuclearcytoplasmic interactions in the mouse embryo. J. Embryol. Exp. Morph. Suppl., 97:277-289
34	Tsunoda, Y., Tokunaga, T. and Sugie, T. 1985. Altered sex ratio of live young after transfer of fast and slow developing mouse embryos. Gamete Res., 12:301-304 DOI
35	Petters, R. M. and Wells, K. D. 1993. Culture of pig embryos. J. Reprod. Fertil. Suppl., 48:61-73 PUBMED
36	Baguisi, A., Behboodi, E., Melican, D. T., Pollock, J. S., Destrempes, M. M., Cammuso, C., Williams, J. L., Nims, S. D., Porter, C. A, Midura, P., Palacios, M. J., Ayres, S. L., Denniston, R. S., Hayes, M. L., Ziomek, C. A, Meade, H. M., Godke, R. A, Gavin, W. ., Overstrom, E. W. and Echelard, Y. 1999. Production of goats by somatic cell nuclear transfer. Nat. Biotechnol., 17:456-461.