한국가축번식학회지 (Korean Journal of Animal Reproduction)

한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Chromosome Aberrations in Porcine Embryo Produced by Nuclear Transfer with Somatic Cell

  • K. S. Chung (Animal Resource Research Center, Konkuk University) ;
  • Ko, S. A (Animal Resource Research Center, Konkuk University) ;
  • S. J. Song (Animal Resource Research Center, Konkuk University SamSung Cheil Hospital, lab of Reproductive Biology and infertility) ;
  • J. T. Do (College of Visual Image & Health, Kongju National University & Research Center for Transgenic Cloned Pigs.) ;
  • Park, Y. S. (SamSung Cheil Hospital, lab of Reproductive Biology and infertility) ;
  • Lee, H. T. (Department of National Science & technology, Seoul National University)
  • 발행 : 2002.12.01
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초록

This study was constructed the correlations of the embryonic developmental rates and the frequency of chromosome aberration using ear-skin-fibroblast cell in nuclear transfer (NT) derived embryos. Karyoplast-oocyte complexes were fused and activated simultaneously, then cultured for seven days to assess development. The developmental rates of NT and in vitro fertilization (IVF) embryos were 55.4% vs 63.5%, 31.7% vs 33% and 13.4% vs 16.8% in 2 cell, 8 cell and blastocyst, respectively. Firstly, the frequency of chromosome aberrations were evaluated using fluorescent in situ hybridization (FISH) technique with porcine chromosome 1 submetacentric specific probe. Chromosome aberration was detected at day 3 on the embryo culture, the percentages of chromosomal aneuploidy in NT and IVF embryos at 4-cell stage were 40%, 31.3%, respectively. Secondly, embryonic fragmentation was evaluated at 4-cell stage embryo. Frequency of embryonic fragmentations was in 51.3% of NT, 61.3% of IVF, 28.9% of parthenogenetic activation at 4-cell stage. The proportion of fragmentation in NT embryos was higher than activation embryos. This result indicates that chromosomal abnormalities and embryonic fragments are associated with low developmental rate in porcine NT embryo. It is also suggest that abnormal porcine embryos produced by NT related with lower implantation rate, increased abortion rate and production of abnormal fetuses.

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참고문헌

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