• 대한미생물학회지
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• 제35권3호
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• pp.263-271
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• 2000

A clinical isolate of Klebsiella pneumoniae K7746 produced the extended-spectrum ${\beta}$-lactamase (ESBL) SHV-12. A 6.6 kb BamHI fragment containing the $bla_{SHV-12}$ gene of K7746 strain was cloned into pCRScriptCAM vector resulting in the recombinant plasmid p7746-Cl. The restriction map of 3.6 kb inserted DNA and sequences immediately surrounding $bla_{SHV-12}$ of p7746-C1 were homologous to plasmid pMPA2a carrying $bla_{SHV-2a}$. In addition, both $bla_{SHV-12}$ and $bla_{SHV-2a}$ were expressed from a common hybrid promoter made of the -35 region derived from the left inverted repeat of IS26 and the -10 region from the $bla_{SHV}$ promoter itself. The results indicate that $bla_{SHV-12}$ and $bla_{SHV-2a}$ may have evolved from a common ancestor in the sequential order of $bla_{SHV-2a}$ first, followed by $bla_{SHV-12}$. Furthermore, by the PCR mapping method using primers corresponding to the IS26 and $bla_{SHV}$, the association between IS26 and $bla_{SHV}$ was studied in 12 clinical isolates carrying $bla_{SHV-2a}$, 27 clinical isolates carrying $bla_{SHV-12}$, and 5 reference strains carrying $bla_{SHV-1}$ to $bla_{SHV-5}$. All 39 strains carrying $bla_{SHV-2a}$ or $bla_{SHV-12}$ were positive by the PCR, providing confirmative evidence that IS26 has been involved in the evolution and dissemination of $bla_{SHV-2a}$ and $bla_{SHV-12}$. But 5 reference strains carrying $bla_{SHV-1}$ to $bla_{SHV-5}$ were negative by the PCR. Therefore, we concluded that the molecular evolutionary pathway of $bla_{SHV-2a}$ and $bla_{SHV-12}$ may be different from that of other $bla_{SHV-ESBL}$, e.g., $bla_{SHV-2}$, $bla_{SHV-3}$, $bla_{SHV-4}$, and $bla_{SHV-5}$.

• Molecules and Cells
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• 제28권4호
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• pp.383-388
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• 2009

The dehydration responsive element binding protein 2C (DREB2C) is a dehydration responsive element/C-repeat (DRE/CRT)-motif binding transcription factor that induced by mild heat stress. Previous experiments established that overexpression of DREB2C cDNA driven by the cauliflower mosaic virus 35S promoter (35S:DREB2C) resulted in increased heat tolerance in Arabidopsis. We first analyzed the proteomic profiles in wild-type and 35S:DREB2C plants at a normal temperature ($22^{\circ}C$), but could not detect any differences between the proteomes of wild-type and 35S: DREB2C plants. The transcript level of DREB2C in 35S: DREB2C plants after treatment with mild heat stress was increased more than two times compared with expression in 35S:DREB2C plants under unstressed condition. A proteomic approach was used to decipher the molecular mechanisms underlying thermotolerance in 35S:DREB2C Arabidopsis plants. Eleven protein spots were identified as being differentially regulated in 35S:DREB2C plants. Moreover, in silico motif analysis showed that peptidyl-prolyl isomerase ROC4, glutathione transferase 8, pyridoxal biosynthesis protein PDX1, and elongation factor Tu contained one or more DRE/CRT motifs. To our knowledge, this study is the first to identify possible targets of DREB2C transcription factors at the protein level. The proteomic results were in agreement with transcriptional data.

• Molecules and Cells
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• 제26권5호
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• pp.496-502
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• 2008

Cryparin, encoded as a single copy gene (Crp) of the chestnut blight fungus Cryphonectria parasitica, is the most abundant protein produced by this fungus. However, its accumulation is decreased remarkably in C. parastica strains containing the double-stranded (ds) RNA virus Cryphonectria hypovirus 1. To characterize the transcriptional regulatory element(s) for strong expression and viral regulation, promoter analysis was conducted. Serial deletion of the Crp promoter region resulted in a step-wise decrease in promoter activity, indicating a localized distribution of genetic elements in the cryparin promoter. Promoter analysis indicated two positive and a repressive cis-acting elements. Among them, the promoter region between nt -1,282 and -907 appeared to be necessary for hypoviral-mediated down-regulation. An electrophoretic mobility shift assay (EMSA) on the corresponding promoter region (-1,282/-907) indicated two regions at (-1,257/-1,158) and (-1,107/-1,008) with the characteristic AGGAGGA-N42-GAGAGGA and its inverted repeat TCCTCTC-N54-TCCTCCT, respectively, appeared to be specific binding sites for cellular factors.

• BMB Reports
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• 제53권5호
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• pp.248-253
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• 2020

Gene expression in HIV-1 is regulated by the promoters in 5' long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed that virus production can be increased by YY1 over-expression and decreased under YY1 depleted condition by siRNA treatment. To identify functional domain(s) of YY1 activation, we constructed a number of YY1 truncated mutants. Our data show that full-length YY1 enhances the viral transcription both through U3 and U3RU5 promoters. Moreover, the C-terminal region (296-414 residues) of YY1 is responsible for the transcriptional upregulation, which could be enhanced further in the presence of the viral Tat protein. The central domain of YY1 (155-295 residues) does not affect LTR activity but has a negative effect on HIV-1 gene expression. Taken together, our study shows that YY1 could act as a transcriptional activator in HIV-1 replication, at least in the early stages of infection.

• 식물조직배양학회지
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• 제22권5호
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• pp.241-260
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• 1995

Transposons, present in the genomes of all living organisms, are genetic element that can change positions, or transpose, within the genome. Most genomes contain several kinds of transposable elements and the molecular details of the mechanisms by which these transposons move have recently been uncovered in many families of transposable elements. Transposition is brought about by an enzyme known as transposaese encoded by the autonomous transposon itself, but, in the unautonomous transposon lacking the gene encoding the transposase, movement occurs only at the presence of the enzyme encoded by the autonomous one. There are two types of transposition events, conservative and replicative transposition. In the former the transposon moves without replication, both strands of the DNA moving together from one place to the other while in the latter the transposition frequently involves DNA replication, so one copy of transposon remains at its original site as another copy insole to a new site. The insertion of transposon into a gene can prevent it expression whereas excision from the gene may restore the ability of the gene to be expressed. There are marked similarities between transposons and certain viruses having single stranded Plus (+) RNA genomes. Retrotransposons, which differ from the ordinary transposons in that they transpose via an RNA-intermediate, behave much like retroviruses and have a structure of integrated retrovial DNA when they are inserted to a new target site. An insertional mutagenesis called transposon-tagging is now being used in a number of plant species to isolate genes involved in developmental and metabolic processes which have been proven difficult to approach by the traditional methods. Attempts to device a transposon-tagging system based on the maize Ac for use in heterologous species have been made by many research workers.

• 한국의류산업학회지
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• 제16권1호
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• pp.43-54
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• 2014

In textile industry, needs for various weave drafts have been increased to produce high qualified textile goods. One of disadvantages of traditional textile industry was spending time and money on manual sampling. Nowadays, however, weave draft design and sampling using CAD programs reduce these consumption efficiently. Therefore, this study aimed to provide high qualified woven fabrics by weave draft designs influenced by geometric patterns. First, We analyzed geometric patterns, except for dot, stripe, and checks, in fashion collections from 2009 to 2014 S/S. Then, based on these analyses, design concepts were decided. Third, weave drafts influenced by geometric patterns were designed with weave CAD program, TEX PRO 10.0 by Youngwoo CNI inc. Forth, We simulated fabrics woven by new drafts using CAD programs, depending on fibers, yarns, density of woven, colors, and finishes. Unclassified geometric patterns would be expressed by small size patterns that influenced by retro moods, square patterns with various color variation, zigzag lines, and pieces of puzzles. Three design concepts were decided as greenness, neoclassic, and romantic chic. Thus, geometric patterns for printing were created as drafts for general looms, and one repeat of each draft were provided. According to the design concepts, we designed 13 fabrics with 4 geometric patterns weaving drafts. All Drafts were designed with CAD programs. Finally, same drafts were simulated as woven fabrics for both S/S and F/W seasons by changing each element, such as fiber, yarns, density, colors, and finishes.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2003년도 춘계종합학술대회논문집
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• pp.160-164
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• 2003

현재 우리나라의 방송환경은 지난 수십년간 지속되어온 아날로그 방송시스템에서 디지털 방송시스템으로 변모해 가는 과도기를 맞고 있다. 이러한 방송환경의 변화와 더불어 디지털 방송 인프라에 담길 각종 디지털 방송 콘텐츠 산업과 고부가가치의 디지털 방송 콘텐츠에 대한 불법 복제 방지 기술에 대한 관심이 고조되고 있다. 디지털방송 환경으로 전환됨에 따라 복제방지에 대한 관심이 민감하게 대두되는 데에는 이유가 있다. 첫 번4, 아날로그 방송환경에서는 전송시의 오류를 수신기에서 완벽하게 제거할 수 없으나 디지털방송 환경의 경우 오류정정 기능에 의해 원본과 동일한 콘텐츠를 수신기에서 복원할 수 있다. 두 번째로는 아날로그의 경우 복제를 반복할수록 복사본의 품질이 원본에 비해 저하되어 가지만, 디지털 의 경우 원본과 동일한 품질을 유지하면서 복사의 횟수와 상관없이 무수히 많은 복사가 가능하다는 점이다. 그리고 세 번째 요소로서 아날로그와 달리 디지털 콘텐츠의 경우 인터넷의 발달과 더불어 온라인으로 누구에게나 손쉽게 전달이 가능하다는 점이다. 이러한 배경 하에서, 본 논문에서는 Triple DES 알고리즘을 이용하여 디지털 콘텐츠 불법 복제 및 배포를 방지하기 위한 디지털 콘텐츠 보호 시스템을 설계 및 구현하고자 한다.

• 통상정보연구
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• 제9권2호
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• pp.103-120
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• 2007

The knowledge based economy requires more and more people to learn new knowledge and skills in a timely and effective manner. These needs and new technology such as computer and Internet are fueling a transition in e-learning. According to specialist's opinion, imagination experience studying is generalized, and learning environment that language barrier by studying, multi-language studying Machine that experience past things that disappear through simulation, and travel area, and experience future changed state disappears is forecasting to come. This is previewing finally that it may become future education that education and IT, element of entertainment is combined. Already, became story that argument for party satellite of e-Learning existence passes one season already. e-Learning is utilized already in all educations that we touch by effectiveness by corporation's competitive power improvement and implement of lifelong education in educational institutions through present e-Learning. It is obvious that when see from our viewpoint which is defining e-Learning by one industry and rear by application to education as well as one new growth power about these, e-Learning industry becomes very important means that can solve dilemma of growth real form. Only, special quality of digital industry that e-Learning is being same with other digital industry and repeat putting out a fire rapidly, and is repeating sudden change that these evolution is not gradual growth of accumulation and improvement of technology that is appearing consider need to. In the meantime, we need to observe about evolution of Information Technology. Because there is some scholars who e-Learning's concept foresees to evolve by u-Learning.(although, a person who see that these concept is not more in marketing terminology by some scholars' opinion is). This u-Learning's concept means e-Learning that take advantage of ubiquitous technology as Ubiquitous-Learning's curtailment speech. Ubiquitous, user means Information-Communication surrounding that can connect to network freely regardless of place without feeling network or computer. There is controversy about introduction time regarding these direction, but e-Learning is judged to evolve by u-Learning necessarily. Because keep in step and age that study all contents that learner wants under environment of 3A (any time, any whrer, any device) by individual order thoroughly is foreseen to come in ubiquitous learning environment that approach more festinately.

• Genomics & Informatics
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• 제3권4호
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• pp.133-141
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• 2005

Most of the endogenous retroviral genes integrated into the primate genome after the split of New World monkeys in the Oligocene era, approximately 33 million years ago. Because they can change the structure of adjacent genes and move between and within chromosomes they may play important roles in evolutionas well as in many kinds of disease and the creation of genetic polymorphism. Comparative analysis of HERVs (human endogenous retroviruses) and their LTR (long terminal repeat) elements in the primate genomes will help us to understand the possible impact of HERV elements in the evolution and phylogeny of primates. For example, HERV-K LTR and SINE-R elements have been identified that have been subject to recent change in the course of primate evolution. They are specific elements to the human genome and could be related to biological function. The HERV-M element is related to the superfamily of HERV-K and is integrated into the periphilin gene as the truncated form, 5'LTR-gag-pol-3'LTR. PCR and RT-PCR approaches indicated that the insertion of various retrotransposable elements in a common ancestor genome may make different transcript variants in different primate species. Examination of the HERV-W elementrevealed that env fragments were detected on human chromosomes 1, 3-7, 12, 14, 17, 20, and X, whilst the pol fragments were detected on human chromosomes 2-8, 10-15, 20, 21, X, and Y. Bioinformatic blast search showed that almost full-length of the HERV-W family was identified on human chromosomes 1-8, 11-15, 17, 18, 21, and X. Expression analysis of HERV-W genes (gag, pol, and env) in human tissues by RT-PCR indicated that gag and pol were expressed in specific tissues, whilst env was constituitively expressed in all tissues examined. DNA sequence based phylogenetic analysis indicated that the gag, pol and env genes have evolved independently during primate evolution. It will thus be of considerable interest to expand the current HERV gene information of various primates and disease tissues.

• 한국자원식물학회지
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• 제16권3호
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• pp.238-244
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• 2003

더덕 뿌리에서 유래한 EST 라이브러리로부터 dehydrin 유전자와 높은 상동성 을 나타내는 full clone cDNA를 얻었다. 더덕의 dehydrin, ClDhn1은 893 bp의 cDNA로 159개의 아미노산을 코딩하는 480 bp의 ORF를 가지고 있다. ClDhn1의 아미노산을 분석해 보면, 전체적으로 높은 친수성을 나타내며, lysine이 풍부한 K 반복구간(KIKEKLPG)을 카르보닐기 쪽에 2개 가지고 있다. 또한, 여러 dehydrin들의 공통적인 특징인 7개의 연속적인 serine잔기가 첫 번째 K반복 구간 앞에 위치한다. 그러나, 아미노기쪽의 DEYGNP보존 구간은 변형(DEHGNP)되어 있다. ClDhn1 유전자는 전사 단계에서 더덕의 뿌리에서 가장 높은 발현 양상을 보이며, 줄기와 잎에서는 적은 양이 발현되었다.