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http://dx.doi.org/10.5483/BMBRep.2020.53.5.222

Human transcription factor YY1 could upregulate the HIV-1 gene expression  

Yu, Kyung Lee (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea)
Jung, Yu Mi (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea)
Park, Seong Hyun (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea)
Lee, Seong Deok (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea)
You, Ji Chang (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea)
Publication Information
BMB Reports / v.53, no.5, 2020 , pp. 248-253 More about this Journal
Abstract
Gene expression in HIV-1 is regulated by the promoters in 5' long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed that virus production can be increased by YY1 over-expression and decreased under YY1 depleted condition by siRNA treatment. To identify functional domain(s) of YY1 activation, we constructed a number of YY1 truncated mutants. Our data show that full-length YY1 enhances the viral transcription both through U3 and U3RU5 promoters. Moreover, the C-terminal region (296-414 residues) of YY1 is responsible for the transcriptional upregulation, which could be enhanced further in the presence of the viral Tat protein. The central domain of YY1 (155-295 residues) does not affect LTR activity but has a negative effect on HIV-1 gene expression. Taken together, our study shows that YY1 could act as a transcriptional activator in HIV-1 replication, at least in the early stages of infection.
Keywords
HIV-1; Tat; transcription regulation; YY1;
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1 Romerio F, Gabriel MN and Margolis DM (1997) Repression of human immunodeficiency virus type 1 through the novel cooperation of human factors YY1 and LSF. J Virol 71, 9375-9382   DOI
2 Bernhard W, Barreto K, Raithatha S and Sadowski I (2013) An upstream YY1 binding site on the HIV-1 LTR contributes to latent infection. PLoS One 8, e77052   DOI
3 Gaynor R (1992) Cellular transcription factors involved in the regulation of HIV-1 gene expression. AIDS 6, 347-363   DOI
4 Pereira LA, Bentley K, Peeters A, Churchill MJ and Deacon NJ (2000) A compilation of cellular transcription factor interactions with the HIV-1 LTR promoter. Nucleic Acids Res 28, 663-668   DOI
5 Jakobovits A, Smith DH, Jakobovits EB and Capon DJ (1988) A discrete element 3' of human immunodeficiency virus 1 (HIV-1) and HIV-2 mRNA initiation sites mediates transcriptional activation by an HIV trans activator. Mol Cell Biol 8, 2555-2561   DOI
6 Boris-Lawrie KA, Brady JN and Kumar A (1992) Sequences within the R region of the long terminal repeat activate basal transcription from the HIV-1 promoter. Gene Expr 2, 215-230
7 Rizkallah R and Hurt MM (2009) Regulation of the transcription factor YY1 in mitosis through phosphorylation of its DNA-binding domain. Mol Biol Cell 20, 4766-4776   DOI
8 Ylisastigui L, Kaur R, Johnson H et al (2005) Mitogenactivated protein kinases regulate LSF occupancy at the human immunodeficiency virus type 1 promoter. J Virol 79, 5952-5962   DOI
9 Barton K and Margolis D (2013) Selective targeting of the repressive transcription factors YY1 and cMyc to disrupt quiescent human immunodeficiency viruses. AIDS Res Hum Retroviruses 29, 289-298   DOI
10 Ott M, Schnolzer M, Garnica J et al (1999) Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity. Curr Biol 9, 1489-1492   DOI
11 Wu S, Shi Y, Mulligan P et al (2007) A YY1-INO80 complex regulates genomic stability through homologous recombination-based repair. Nat Struct Mol Biol 14, 1165-1172   DOI
12 Dorr A, Kiermer V, Pedal A et al (2002) Transcriptional synergy between Tat and PCAF is dependent on the binding of acetylated Tat to the PCAF bromodomain. EMBO J 21, 2715-2723   DOI
13 Atchison L, Ghias A, Wilkinson F, Bonini N and Atchison ML (2003) Transcription factor YY1 functions as a PcG protein in vivo. EMBO J 22, 1347-1358   DOI
14 Bushmeyer S, Park K and Atchison ML (1995) Characterization of functional domains within the multifunctional transcription factor, YY1. J Biol Chem 270, 30213-30220   DOI
15 Austen M, Luscher B and Luscher-Firzlaff JM (1997) Characterization of the transcriptional regulator YY1. The bipartite transactivation domain is independent of interaction with the TATA box-binding protein, transcription factor IIB, TAFII55, or cAMP-responsive element-binding protein (CPB)-binding protein. J Biol Chem 272, 1709-1717   DOI
16 Wilkinson FH, Park K and Atchison ML (2006) Polycomb recruitment to DNA in vivo by the YY1 REPO domain. Proc Natl Acad Sci U S A 103, 19296-19301   DOI
17 Shi Y, Seto E, Chang LS and Shenk T (1991) Transcriptional repression by YY1, a human GLI-Kruppel-related protein, and relief of repression by adenovirus E1A protein. Cell 67, 377-388   DOI
18 Seto E, Shi Y and Shenk T (1991) YY1 is an initiator sequence-binding protein that directs and activates transcription in vitro. Nature 354, 241-245   DOI
19 Park K and Atchison ML (1991) Isolation of a candidate repressor/activator, NF-E1 (YY-1, delta), that binds to the immunoglobulin kappa 3' enhancer and the immunoglobulin heavy-chain mu E1 site. Proc Natl Acad Sci U S A 88, 9804-9808   DOI
20 Donohoe ME, Zhang X, McGinnis L, Biggers J, Li E and Shi Y (1999) Targeted disruption of mouse Yin Yang 1 transcription factor results in peri-implantation lethality. Mol Cell Biol 19, 7237-7244   DOI
21 Gordon S, Akopyan G, Garban H and Bonavida B (2006) Transcription factor YY1: structure, function, and therapeutic implications in cancer biology. Oncogene 25, 1125-1142   DOI
22 Thomas MJ and Seto E (1999) Unlocking the mechanisms of transcription factor YY1: are chromatin modifying enzymes the key? Gene 236, 197-208   DOI
23 Satijn DP, Hamer KM, den Blaauwen J and Otte AP (2001) The polycomb group protein EED interacts with YY1, and both proteins induce neural tissue in Xenopus embryos. Mol Cell Biol 21, 1360-1369   DOI
24 Shi Y, Lee JS and Galvin KM (1997) Everything you have ever wanted to know about Yin Yang 1. Biochim Biophys Acta 1332, F49-66
25 Hyde-DeRuyscher RP, Jennings E and Shenk T (1995) DNA binding sites for the transcriptional activator/repressor YY1. Nucleic Acids Res 23, 4457-4465   DOI
26 Lee JS, Galvin KM, See RH et al (1995) Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300. Genes Dev 9, 1188-1198   DOI
27 Yao YL, Yang WM and Seto E (2001) Regulation of transcription factor YY1 by acetylation and deacetylation. Mol Cell Biol 21, 5979-5991   DOI
28 Coull JJ, Romerio F, Sun JM et al (2000) The human factors YY1 and LSF repress the human immunodeficiency virus type 1 long terminal repeat via recruitment of histone deacetylase 1. J Virol 74, 6790-6799   DOI
29 Ko CY, Hsu HC, Shen MR, Chang WC and Wang JM (2008) Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex. J Biol Chem 283, 30919-30932   DOI
30 Margolis DM, Somasundaran M and Green MR (1994) Human transcription factor YY1 represses human immunodeficiency virus type 1 transcription and virion production. J Virol 68, 905-910   DOI
31 He G and Margolis DM (2002) Counterregulation of chromatin deacetylation and histone deacetylase occupancy at the integrated promoter of human immunodeficiency virus type 1 (HIV-1) by the HIV-1 repressor YY1 and HIV-1 activator Tat. Mol Cell Biol 22, 2965-2973   DOI