• BMB Reports
• /
• v.51 no.9
• /
• pp.437-443
• /
• 2018

Non-homologous end joining (NHEJ), and to a lesser extent, the error-free pathway known as homology-directed repair (HDR) are cellular mechanisms for recovery from double-strand DNA breaks (DSB) induced by RNA-guided programmable nuclease CRISPR/Cas. Since NHEJ is equivalent to using a duck tape to stick two pieces of metals together, the outcome of this repair mechanism is prone to error. Any out-of-frame mutations or premature stop codons resulting from NHEJ repair mechanism are extremely handy for loss-of-function studies. Substitution of a mutation on the genome with the correct exogenous repair DNA requires coordination via an error-free HDR, for targeted transgenesis. However, several practical limitations exist in harnessing the potential of HDR to replace a faulty mutation for therapeutic purposes in all cell types and more so in somatic cells. In germ cells after the DSB, copying occurs from the homologous chromosome, which increases the chances of incorporation of exogenous DNA with some degree of homology into the genome compared with somatic cells where copying from the identical sister chromatid is always preferred. This review summarizes several strategies that have been implemented to increase the frequency of HDR with a focus on somatic cells. It also highlights the limitations of this technology in gene therapy and suggests specific solutions to circumvent those barriers.

• Journal of Conservation Science
• /
• v.19
• /
• pp.31-42
• /
• 2006

There are many materials used in restoring missing parts of pottery and ceramics and they are used according to the each quality of materials. In our research we tried to find the new repair materials to solve many problems exposed in the existing repair materials. As a result, we made new repair materials that we properly put oven bake clays into repair epoxy putty which is being used as oven bake clays for a long time. Fortunately, through testing yellowing, vickers hardness, coloring and heating temperature, We could get an idea that new repair materials would be the best materials in the efficiency of restoration, yellowing, and coloring.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.11 no.12
• /
• pp.5729-5744
• /
• 2017

Vehicular Ad Hoc Networks (VANET) in the large-scale road section usually have typical characteristics of large number of vehicles and unevenly distribution over geographic spaces. These two inherent characteristics lead to the unsatisfactory performance of VANETs. This poor performance is mainly due to fragile communication link and low dissemination efficiency. We propose a novel routing mechanism to address the issue in the paper, which includes a competition-based routing discovery with priority metrics and a local routing repair strategy. In the routing discovery stage, the algorithm uses adaptive scheme to select a stable route by the priorities of routing metrics, which are the length of each hop, as well as the residual lifetime of each link. Comparisons of different ratios over link length and link stability further show outstanding improvements. In the routing repair process, upstream and downstream nodes also compete for the right to establish repair process and to remain as a member of the active route after repair. Our simulation results confirm the improved performance of the proposed algorithm.

• Steel and Composite Structures
• /
• v.32 no.1
• /
• pp.127-139
• /
• 2019

Bridges, offshore oil platforms and other infrastructures usually require at some point in their service life rehabilitation for reasons such as aging and corrosion. This study explores the application of adhesively bonded CFRP patches in repair of corroded circular hollow sectional (CHS) steel beams. An experimental program involving three-point bending tests was conducted on intact, corroded, and repaired CHS beams. Meso-scale finite element (FE) models of the tested beams were developed and validated by the experimental results. A parametric study using the validated FE models was performed to examine the effects of different CFRP patch parameters, including patch dimensions, number of plies and stacking sequence, on efficiency of the repair system. Results indicates that the corrosion reduced elastic stiffness and flexural strength of the undamaged beam by 8.9 and 15.1%, respectively, and composite repair recovered 10.7 and 18.9% of those, respectively, compared to undamaged beam. These findings demonstrated the ability of CFRP patch repair to restore full bending capacity of the corroded CHS steel beam. The parametric study revealed that strength and stiffness of the repaired CHS beam can be enhanced by changing the fiber orientations of wet composite patch without increasing the quantity of repair materials.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2011.11a
• /
• pp.147-148
• /
• 2011

This study applied BIM(Building Information Modeling) technology for Long-life Housing within exterior, interior and building equipment. There has many changes and depression infill material after construction. Therefore to understand establishment of repair and replacement cycle is necessity. In addition, the method of classification is necessary because of construction equipment efficiency. On this study, we will find how can we manage them and establish the repair and repairment cycle by applying BIM technology.

• Journal of Advanced Marine Engineering and Technology
• /
• v.31 no.6
• /
• pp.810-817
• /
• 2007

Every ports is making great efforts to get competitiveness. It is progressing for the research on port model of new concept that connect port-related business to be developed from base running simple cargo processing to universal port that has economical efficiency and competitiveness, This research shows development way of Busan port that connect ship-repair industry which is port-related business. Development way is especially handled link between port and ship-repair industry field activation of high value-added ship as LNG etc.

• Structural Engineering and Mechanics
• /
• v.59 no.1
• /
• pp.123-132
• /
• 2016

Many military and commercial aging aircrafts flying beyond their design life may experience severe crack and corrosion damage, and thus lead to catastrophic failures. In this paper, were used in a finite element model to evaluate the effect of corrosion on the adhesive damage in bonded composite repair of aircraft structures. The damage zone theory was implemented in the finite element code in order to achieve this objective. In addition, the effect of the corrosion, on the repair efficiency. Four different patch shapes were chosen to analyze the adhesive damage: rectangular, trapezoidal, circular and elliptical. The modified damage zone theory was implemented in the FE code to evaluate the adhesive damage. The obtained results show that the adhesive damage localized on the level of corrosion and in the sides of patch, and the rectangular patch offers high safety it reduces considerably the risk of the adhesive failure.

• BMB Reports
• /
• v.41 no.3
• /
• pp.230-235
• /
• 2008

LRP15 is a novel gene cloned from lymphocytic cells, and its function is still unknown. Bioinformatic data showed that LRP15 might be regulated by DNA methylation and had an important role in DNA repair. In this study, we investigate whether the expression of LRP15 is regulated by DNA methylation, and whether overexpression of LRP15 increases efficiency of DNA repair of UV-induced DNA damage in HeLa cells. The results showed (1) the promoter of LRP15 was hypermethylated in HeLa cells, resulting a silence of its expression. Gene expression was restored by a demethylating agent, 5-aza-2'-deoxycytidine, but not by a histone deacetylase inhibitor, trichostatin A; (2) overexpression of LRP15 inhibited HeLa cell proliferation, and the numbers of cells in the G2/M phase of the cell cycle in cells transfected with LRP15 increased about 10% compared with controls; (3) cyclin B1 level was much lower in cells overexpressing LRP15 than in control cells; and (4) after exposure to UV radiation, the LRP15-positive cells showed shorter comet tails compared with the LRP15-negative cells. From these results we conclude that the expression of LRP15 is controlled by methylation in its promoter in HeLa cells, and LRP15 confers resistance to UV damage and accelerates the DNA repair rate.

• Journal of Korean Society for Quality Management
• /
• v.49 no.2
• /
• pp.213-231
• /
• 2021

Purpose: This study intends to provide decision-making information to improve efficiency by analyzing the management efficiency of smart greenhouse business entities and identifying factors that affect the efficiency based on input and output. Methods: The subjects of analysis were business entities for cultivating strawberries in smart greenhouses in Jeolla region (northern and southern Jeolla provinces), and the analysis focused on the management performance of the 2019-2020 crop period (year). Data Envelopment Analysis(DEA) was applied as an analysis method for efficiency analysis, Quantile Regression(QR) analysis was applied as a factor affecting the efficiency. Results: The reason for the efficiency gap between business entities was that there were many business entities that did not minimize the input cost at the current level of output, and the area where the variance among business entities was large was the fixed cost per 10a. In the results of the affecting factor analysis, it was found that the seed-seedlings cost, fertilizer cost, other material cost, and employment and labor cost had a negative (-) effect on the efficiency, and that the repair and maintenance cost had a positive (+) effect. Conclusion: Therefore, to achieve the efficiency of scale, it is necessary to reduce the input scale to an appropriate level. In the case of business entities with low efficiency by quartile, the seed-seedlings, fertilizer, and other material costs reduce expenditures, and repair maintenance costs can improve efficiency by increasing expenditures.

• Journal of Plant Biotechnology
• /
• v.42 no.3
• /
• pp.154-160
• /
• 2015

The plant breeding technology was developed with genetic engineering. Many researchers and breeders have turned from traditional breeding to molecular breeding. Genetically modified organisms (GMO) were developed via molecular breeding technology. Currently, molecular breeding technologies facilitate efficient plant breeding without introducing foreign genes, in virtue by of gene editing technology. Gene targeting (GT) via homologous recombination (HR) is one of the best gene editing methods available to modify specific DNA sequences in genomes. GT utilizes DNA repair pathways. Thus, DNA repair systems are controlled to enhance HR processing. Engineered sequence specific endonucleases were applied to improve GT efficiency. Engineered sequence specific endonucleases like the zinc finger nuclease (ZFN), TAL effector nuclease (TALEN), and CRISPR-Cas9 create DNA double-strand breaks (DSB) that can stimulate HR at a target site. RecQl4, Exo1 and Rad51 are effectors that enhance DSB repair via the HR pathway. This review focuses on recent developments in engineered sequence specific endonucleases and ways to improve the efficiency of GT via HR effectors in plants.