• BMB Reports
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• 제53권7호
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• pp.341-348
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• 2020

The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.652-657
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• 2008

A stress distribution of composite laminates patches is obtained by using the Kantorovich method when the substrate is under uniaxial load. The analysis is based on the stress function approach and uses the complementary virtual work principle. The three-dimensional stresses satisfy the traction free conditions at the free edges and the top surfaces of the patch. The stress of the bottom surfaces of the patch is obtained from equilibrium equation of patch and substrate. To demonstrate the efficiency and validity of the proposed analysis, numerical examples for cross-ply and quasi-isotropic laminates are included. The present method provides accurate stresses in the interior and near the free edges of composite laminate patches.

• KIEAE Journal
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• 제2권4호
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• pp.57-64
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• 2002

Nowadays the importance of Building Remodeling as a means to create an architectural environment capable of responding to the social, economic changes has increased more than before. After long time pass, the aged building have faced the need for the overall repair of the structure and function. Remodeling is much more saving the time and cost than new construction in the office building. This study was focused in functional and efficient evaluation. Based on these points, we developed the evaluation factors, and verified a validity of evaluation factors through a survey. The purpose of this study was focused on the Analysis for Recognizing the priority of Indoor Environmental Factors in office Remodeling. This study provides us the competitive guidance of remodeling. The owners and tenants take the maximum efficiency with the minimum cost.

• Journal of Computational Design and Engineering
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• 제2권2호
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• pp.119-127
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• 2015

In the case of a high-valuable asset, the Operation and Maintenance (O&M) phase requires heavy charges and more efforts than the installation (construction) phase, because it has long usage life and any accident of an asset during this period causes catastrophic damage to an industry. Recently, with the advent of emerging Information Communication Technologies (ICTs), we can get the visibility of asset status information during its usage period. It gives us new challenging issues for improving the efficiency of asset operations. One issue is to implement the Condition-Based Maintenance (CBM) approach that makes a diagnosis of the asset status based on wire or wireless monitored data, predicts the assets abnormality, and executes suitable maintenance actions such as repair and replacement before serious problems happen. In this study, we have addressed several aspects of CBM approach: definition, related international standards, procedure, and techniques with the introduction of some relevant case studies that we have carried out.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 A
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• pp.473-475
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• 2003

Owing to the strike of lightning a breakage of insulator would happen. This breakage may give rise to many problems such as increment of reclosing failure, a drop for reliance and a hardship of maintenance and repair. To solve those problem, this study develop a protected equipment for insulator which is suitable to 22.9kV distribution line towers and is purposed to investigation for a proper adaption, protection efficiency of insulator and effect of adaption.

• Smart Structures and Systems
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• 제2권4호
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• pp.305-312
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• 2006

In the case of hospital buildings, where seismic design requirements are very high, existing structuresand especially those attacked by past earthquakes, appear, often, unable to fulfil the necessary safety prerequisites. In this paper, the retrofitting of hospital buildings is investigated, using alternative methods of repair and strengthening. Analysis of an existing hospital building in Patras, Greece, is performed. The load-bearing system is a reinforced concrete system. Two solutions are proposed: strengthening using concrete jackets around column and beam elements and application of viscoelastic dampers for the increase of the stability of the structure. Adequate finite element models are constructed for each case and conclusions are drawn on the efficiency of each rehabilitation method.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2006년도 추계학술대회 논문집
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• pp.885-889
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• 2006

Metro Subway System is widely known as the leader of public transportation in a metropolitan area. The signal interlocking is one of the most important organs that plays a major role in the system. By improving the quality of signal lights on the traffic system and keeping its maintenance on a high level will not only repair the current state, but it will also let the LED type signal semi-permanent. The device, which underwent a process of fine manufacture establishment and a close examination, obtained a patented article (2004, as a new device). Utilizing the equipment with cautious preservation on the system will enhance the current state of the signal device. Especially, the test for improvement and development based upon the technique that decreases the frequency of defect produced will further precipitate its efficiency. With authorization of imposing the LED lights on production, installment, and investigation of the system, the newly made equipment will bring improvement to the signal technology and to the industry at largest extent.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 하계학술대회 논문집 A
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• pp.274-277
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• 1995

Recent environmental pollutions have intensified the need to develop zero emission vehicles. The most effect method of such solutions is EV. EV is high energy efficiency, easy to maintain, repair and is possible to make high performance control. However, because energy density of batteries is constrained and the distance covered one charge is short range. Also because EV has disadvantage of poor accelation ability, development of high performance battery is required for large scale use of EV. EV charger analogous to gas apparatus must also be developed immediately. Charger is discriminate between on-vehicle type and off-vehicle type. As off-vehicle type is able to charge fast and safe, inductive charging is considered. This paper aims to develope off-vehicle inductive charging system. Therefore, it achieved power factor correction converter, high frequency DC/AC inverter control algorithm development which gives proof validity through simulation and formulated the basic concept of high frequency transformer design for inductive charging.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2006년도 하계학술발표대회 논문집
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• pp.820-825
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• 2006

It is necessary to correspond to building users' claims rapidly as much as possible in order to satisfy user's need, because the final tarket of building maintenance service is user of building. This paper investigates the time taken for receiving users' claims and the time taken for the service organization to respond to the claims in the office building; types of claims and service responses are analysed, through which the response characteristics of the maintenance management organization are evaluated with respect to users' claims. Throughout extensive simulation analysis, important performance measures such as mean time to repair and utilization of maintenance personnels have been investigated.

• 전력전자학회논문지
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• 제24권5호
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• pp.356-364
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• 2019

In this study, major problems, such as licensing problems due to civil complaints, deterioration of facility period, and damage of modules, are exposed to many problems in domestic businesses. Particularly, the photovoltaic (PV) modules applied to early PV systems have been repaired and replaced over the past two decades, and a new module-based aging detection method is needed to expand the maintenance market and stabilize and repair power supplies. PV modules in a PV system use a string that is configured in series to generate high voltage. However, even if only one module of the solar modules connected in series ages, the power generation efficiency of the aged string is reduced. Therefore, we propose a topology that can measure the instantaneous PV characteristic curve to determine the aging module in the solar string and the aging judgment algorithm using the measured PV characteristic curve.