• Journal of Life Science
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• v.30 no.7
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• pp.651-659
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• 2020

Cardiovascular disease is one of the leading causes of death across the world, and gold-standard treatments such as percutaneous coronary intervention and artery bypass grafting have various limitations including myocardial damage and subsequent maladaptive cardiac remodeling. To overcome this, stem-cell therapies are emerging as a promising strategy for cardiovascular regeneration. Endothelial progenitor cells (EPCs) have high potential to proliferate and differentiate into endothelial cells for vascularization and tissue regeneration, and several clinical trials have explored EPC function in tissue repair in relation to clinical safety and improving cardiac function. Consequently, EPC has been suggested as a feasible stem-cell therapy. However, autologous EPC transplantation in cardiovascular disease patients is restricted by risk factors such as age, smoking status, and hypertension that lead to reduced bioactivity in the EPCs. New approaches for improving EPC function and stem-cell efficacy have therefore been suggested, including cell priming, organoid culture systems, and enhancing transplantation efficiency through 3D bioprinting methods. In this review, we provide a comprehensive understanding of EPC characteristics, therapeutic approaches, and the current state of clinical research into EPCs as stem-cell therapy for cardiovascular disease.

• Journal of Korean Society of Forest Science
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• v.96 no.4
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• pp.419-424
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• 2007

The urban forest offer many opportunities to maintain and enhance local and regional biological diversity. Pinus densiflora stands in isolated urban forest in five areas of Seoul, Incheon, Daegu, Ulsan, and Cheongju were investigated by landscape and community ecological methods for diagnosis of current status and construction of conservation plan. Species composition of five study areas revealed regional characteristics. As the result of stand dynamics, absence of pine seedlings and saplings were observed. Cheongju, significantly, revealed highest species diversity (H'=1.86) and richness (24) among study areas, and the opposite was true for Daegu (1.35 for H', and 14 for richness). Ratio of alien species was highest in Daegu (15.8%) (F=5.05, p=0.0016). Based on landscape analysis, complexity of pine patch was higher in local city than metropolitans, but statistically not significant (p=0.133). Complexity of pine patch revealed significant positive correlation with diversity and richness, but not with percentage of aliens. Synthetically, ecological management based on community and landscape ecological information was recommended. Cautious artificial regeneration strategy was necessary to regenerate pine forest in urban area.

• Journal of the Korean Institute of Landscape Architecture
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• v.49 no.1
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• pp.19-30
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• 2021

This research studied the effect of visual improvement of "72 Hour Project" that has regenerated small scale derelict spaces in Seoul through citizen participation. 29 projects built form 2016 to 2019 were analyzed. The research analyzed landscape image preference of before and after status of projects using 12 pairs of landscape adjectives. Basic statistical analysis, correlation analysis, factor analysis, cluster analysis, and ANOVA were performed based on the survey results. Since the satisfaction level of the projects compared with the before-condition was 3.63 higher than 3.00, it could be concluded that there was an meaningful effect of visual improvement after completion of the projects. As the result of the factor analysis, landscape adjective pairs were categorized into two factors: harmony and aesthetics. Through the cluster analysis, four clusters were formed and characteristics of each cluster were identified. As the result of rhe cluster analysis, the cluster with the high harmony level and the aesthetics level showed the highest overall satisfaction level. Comparing each cluster, it could be concluded that the factor of harmony was more important than the factor of aesthetics in evaluating the satisfaction level of projects. Analyzing qualitative aspects of project groups, spatially well-balanced design with generous vegetation areas was more effective in landscape improvement than artistic design with visually strong installations. Further researches based on behavior studies of actual users are required to compensate the limits of this research. This research can contribute to establish the improved direction of policies to regenerate various types of small scale derelict spaces.

• Journal of the korean academy of Pediatric Dentistry
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• v.50 no.1
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• pp.1-12
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• 2023

Function of salivary gland and saliva composition can be an indicator of individual's health status. Recently, saliva has been thought to have a high potential for usage in the biomedical field to diagnose, evaluate, and prevent systemic health due to the technological advances in analyzing and detecting small elements such as immunological and metabolic products, viruses, microorganisms, hormones in saliva. As a diagnostic specimen, saliva has some useful advantages compared to serum. Because of simple non-invasive method, saliva sampling is quite comfort for the patient, and it doesn't require specialists to collect samples. The possibility of infection during the collection process is also low. For this reason, proteins, genetic materials, and various biomarkers in saliva are actively being utilized on studying stress, microbiomics, genetics, and epigenetics. For the research on collecting big data related to systemic health, the needs on biobank has been focused. Regeneration of salivary gland based on tissue engineering has been also on advancement. However, there are still many issues to be solved, such as the standardization of sample collection, storage, and usage. This review focuses on the recent trends in the field of saliva research and highlight the future perspectives in biomedical and other applications.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.498-504
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• 2014

Potato virus Y (PVY) and potato leafroll virus (PLRV) are among the most damaging potato viruses and prevalent in most potato growing areas. In this study, cryopreservation was used to eradicate PVY and PLRV using two cryogenic methods. Potato shoot tips proliferated in vitro were cryopreserved through droplet-vitrification and encapsulation-vitrification using plant vitrification solution 2 (PVS2; 30% glycerol + 15% dimethyl sulfoxide + 15.0% ethylene glycol + 13.7% sucrose) and modified PVS2. Both cryogenic procedures produced similar rates of survival and regrowth, which were lower than those from shoot tip culture alone. The health status of plantlets regenerated from shoot tip culture alone and cryopreservation was checked by reverse transcription-polymerase chain reaction. The frequency of virus-free plants regenerated directly from highly proliferating shoot tips reached 42.3% and 48.6% for PVY and PLRV, respectively. In comparison, the frequency of PVY and PLRV eradication after cryopreservation was 91.3~99.7% following shoot-tip culture. The highest cryopreserved shoot tip regeneration rate was observed when shoot tips were 1.0~1.5 mm in length, but virus eradication rates were very similar (96.4~99.7%), regardless of shoot tip size. This efficient cryotherapy protocol developed to eliminate viruses can also be used to prepare potato material for safe long-term preservation and the production of virus-free plants.

• The Journal of the Korea Contents Association
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• v.12 no.3
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• pp.449-460
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• 2012

Since 1970's, the rivers in industrial cities and metropolitan cities in Korea have been severely contaminated and the riverfronts have been to garbage yard, warehouses and sanitary facilities that produce low efficiency in urban land-use. As a result, riverfront in most cities became lost space and artificial area which composed of asphalt road, concrete riverbank and parking lot. However, Sustainable management is the main concept of riverfront development in 21st century. Also, in contemporary riverfront space, it is the pivotal paradigm that the development of eco-space and mixture of cultural space. Citizen require greener, more ecological and water-friendly space in riverfront of city. So, the purpose of this paper lies in suggestion for building sustainable development and management for riverfront in Korean city. For this purpose, this paper has reviewed the developmental trend of recent riverfront, has analyzed locational environment and land use of riverfront in city, has set policy and the strategy for sustainable riverfront.

• Journal of the Korean Institute of Rural Architecture
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• v.19 no.3
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• pp.9-16
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• 2017

The study aims to find regional characteristics of Olle and Olle-Dam the old town of Jeju city, "Mu-geun-sung," through researches of the status of the spatial structure in the area. The research was mainly conducted through literature survey and field survey. Literature survey was conducted for the typification of alleys and stone walls. Field surveys were conducted to reveal the location, length, and materials of the stone walls on alleys. As a result, this study can grasp the spatial characteristics of the alleys and the typological characteristics of the stone walls in the old urban areas of Jeju City. The unit alley located in the old city area of Jeju was severely damaged. Also, the stone walls could reveal that there are few areas where the original shape is preserved. The management of the community has not been properly carried out in the use of alleys. In addition, there is concern about the damage caused by securing a road in the future. On the other hand, many of the damaged stone walls had basically the original shape. There were many stone walls that retained their original shape in the old alley. For this reason, there is a high possibility that the alleys and stone walls located in the old urban areas of Jeju are utilized as assets of the area. Based on the results of the above study, community recovery using old alleys of Jeju is required.

• The Journal of the Korean dental association
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• v.55 no.1
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• pp.30-41
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• 2017

Inadequate keratinized mucosa around dental implants can lead to more plaque accumulation, tissue inflammation, marginal recession and attachment loss. We evaluated the effects of free gingival and extracellular matrix membrane grafts performed to increase the insufficient width of keratinized tissue around dental implants in the posterior mandible. A 47-year-old female patient presented with discomfort due to swelling of the lower right second premolar area. Due to severe destruction of alveolar bone, the tooth was extracted. After 3 months, a guided bone regeneration (GBR) procedure was performed and then a dental implant was placed 6 months later. During the second-stage implant surgery, free gingival grafting was performed to increase the width of the keratinized tissue. After 12 months, a clinical evaluation was performed. A 64-year-old female patient had a missing tooth area of bilateral lower molar region with narrow zone of keratinized gingiva and horizontal alveolar bone loss. Simultaneous implant placement and GBR were performed. Five months after the first-stage implant surgery, a gingival augmentation procedure was performed with an extracellular matrix membrane graft to improve the width of the keratinized tissue in the second-stage implant surgery. After 12 months, a clinical evaluation was performed. In these two clinical cases, 12 months of follow-up, revealed that the increased width of the keratinized tissue and the deepened oral vestibule was well maintained. A patient showed a good oral hygiene status. In conclusion, increased width of keratinized tissue around dental implants could improve oral hygiene and could have positive effects on the long-term stability and survival rate of dental implants. When planning a keratinized tissue augmentation procedure, clinicians should consider patient-reported outcomes.

• Journal of Periodontal and Implant Science
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• v.46 no.1
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• pp.10-21
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• 2016

Purpose: This study evaluated the predictive performance of a combination of self-report questionnaires, salivary hemoglobin levels, and age as a non-invasive screening method for periodontitis. Methods: The periodontitis status of 202 adults was examined using salivary hemoglobin levels, responses to 10 questions on a self-report questionnaire, and the Community Periodontal Index (CPI). The ability of those two variables and the combination thereof with age to predict the presence of CPI scores of 3-4 and 4 was assessed using logistic regression and receiver operating characteristic (ROC) curve analysis. Results: CPI scores of 3-4 and 4 were present among 79.7% and 46.5% of the sample, respectively. The area under the ROC curves (AUROCs) of salivary hemoglobin levels for predicting prevalence of CPI scores of 3-4 and 4 were 0.63 and 0.67, respectively (with sensitivity values of 71% and 60% and specificity values of 56% and 72%, respectively). Two distinct sets of five questions were associated with CPI scores of 3-4 and 4, with AUROCs of 0.73 and 0.71, sensitivity values of 76% and 66%, and specificity values of 63% and 69%. The combined model incorporating both variables and age showed the best predictive performance, with AUROCs of 0.78 and 0.76, sensitivity values of 71% and 65%, and specificity values of 68% and 77% for CPI scores of 3-4 and 4, respectively. Conclusions: The combination of salivary hemoglobin levels and self-report questionnaires was shown to be a valuable screening method for detecting periodontitis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.1
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• pp.399-408
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• 2016

This study conducted an analysis of the current use of and satisfaction with 40 village-unit core facilities that were created through a village-building project in Busan targeting users and non-users of the facilities. The study then examined ideas to promote the availability of the facilities. The results showed that for the users, they had been using the facilities for gathering and information-sharing, and the facilities were improving the interactions among the locals. In terms of non-users, the study confirmed that any relevant fields should develop diverse programs, start promotions in various ways and establish networks among neighbors to increase the non-users' use of the facilities In addition, the study found that the village-unit core facilities are working to encourage interactions among residents and that the locals' voluntary participation in operation of the facilities are needed. The study also suggested that networks across the local communities and diverse programs should be formed and developed.