• Microbiology and Biotechnology Letters
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• v.36 no.3
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• pp.182-188
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• 2008

A hyperthermophilic bacteria (strain HJ6) was isolated from a hot springs located in the Arima-cho, Hyogo, Japan. The cells were long-rod type ($2-4{\mu}m$), about $0.4{\mu}m$ in diameter. The pH and temperature for optimal growth were 6.5 and $80^{\circ}C$, respectively. Phylogenetic analysis based on the 16S rDNA sequence and biochemical studies indicated that HJ6 belonged to the genus Thermus thermophilus (Tt). The gene encoding the Trehalose synthase (TS) was cloned and sequenced. The open reading frame (ORF) of the TtTS gene was composed of 2,898 nucleotides and encoded a protein (975 amino acids) with a predicted molecular weight of 110.56 kDa. The deduced amino acid sequence of TtTS showed 99% and 83% identities to the Thermus caldophilus TS and Meiothermus ruber TS, respectively. TtTS gene was expressed in Escherichia coli cells, and the recombinant protein was purified to homogeneity. The optimal temperature and pH for Trehalose synthase activity were found to be $80^{\circ}C$ and 7.5, respectively. The half-life of heat inactivation was about 40 min at $90^{\circ}C$. The maximum trehalose conversion rate of maltose into trehalose by the enzyme increased as the substrate concentration increased, and reached 55.7% at the maltose concentration of 500 mM, implying that the enzyme conversion was dependent of the substrate concentration.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.24 no.2
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• pp.49-69
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• 2013

The purpose of this study is to analyze status of information service and to suggest its activation strategies after establishing its category in the school library. As a result of establishing scope and contents of information service, materials of information offering and guide are converged on reading education and information literacy curriculum integrated subjects. But the arrival rate of information service is very low like 48.2% under the 2012 Korean Libraries' Management Evaluation. It is especially poor to raise self resilience and school participation of students with information service involved in the affective domain such as readers' advisory service and bibliotherapy. In order to activate its information service, there is an urgent need for systems improvement in placement of the teacher librarian. It is also necessary to reinforce the status of information literacy instruction under the nation-level curriculum and continuous base expansion as a core information service of the school library. Service development for readers' advisory service and bibliotherapy is also needed to contribute to solving problem and stress situation of the student. The school library has to make an effort to connect its information service with local communities for the school community.

• Korean Journal of Digital Imaging in Medicine
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• v.12 no.1
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• pp.33-41
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• 2010

National Cancer Screening Project and Korean Society of Breast Imaging recommend that breast cancer screening should be performed on those aged 40 and above. Nevertheless, this recommendation is usually ignored by a number of medical institutions. The purpose of this study is to emphasize the necessity of an age limitation in screening mammography. Ten institutions were randomly selected and telephone inquiries about patients' age limitation and internal guidelines were set up. The 3,214 women, who underwent screening mammography through 'GE Senography 2000D' in each hospital, were classified into five groups according to age(from 20s to 40s, at intervals of 5). And then, collected data was analyzed by a radiologist in accordance with ACR-BIRADS(American College of Radiology Breast Imaging Reporting and Data System), through which breast parenchymal density and the results of analysis were categorized in order to predict the sensitivity of mammography. Information about craniocaudal-view mammograms was automatically produced by use of GE Senography 2000D, and the average glandular dose was retrospectively analyzed through the program 'Excel 2007.' Two institutions did not set the age limitation. Other seven institutions internally allowed those who wanted to receive mammography regardless of age. Approximately 99% of those aged 20 to 29 were judged as having the dense breast. In those aged 35 to 39, breast parenchymal density tended to be lower, but the fatty breast to increase. In the case of 'category-zero' that does not need additional tests, the rate of 'heterogeneously dense' and 'extremely dense' reached to 83.1% and 15.1% respectively. Regarding dense breasts, there was no sufficient information for image reading. The glandular dose, applied to 3,214, was 1.47mGy on the average. In those aged 20 to 24 who are sensitive to radiation, the average glandular dose indicated 1.59mGy. Those aged 35 and above showed the lowest value, 1.43mGy. In those aged 35 to 39, the breast tended to change from denseness to fattiness. The average glandular dose was lowest in those aged 35 and above, which suggests that screening mammography should be periodically performed on those aged 35 and above in order that breast cancer may be early detected. On the other hand, in those aged less than 35, it is difficult to analyze mammograms due to the high density of breast parenchyma, and also retakes become frequent. In particular, subjects may be exposed to excessive doses. Accordingly, it should be substituted by breast self-examination or clinical breast examination. In case of need, it is advisable to perform ultrasonography.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.7
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• pp.1573-1582
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• 2011

Risk of lung cancer among lung-related diseases has gradually increased during last decades. The chest digital radiography is the primary diagnosis method for lung cancer. Diagnosing lung cancer using this method requires doctors of ripe experience. Despite their experience there are often wrong diagnoses, which decrease early diagnosis and survival rates of patients. The aim of this study was intended to establish the base on the Computer Aided Diagnosis (CAD) by analyzing Image Recognition Algorithm using Principle component Analysis (PCA) and diagnosing patient's chest X-ray image. The database obtained through this approach enables a doctor to significantly reduce misdiagnosis during the early diagnosis stage, if he or she utilizes it as the preliminary reading step. Case studies were carried out using normal organ, and organs suffering from bronchogenic carcinoma and granuloma. A normal image and unique disease images were extracted after PCA analysis, and their cross-recognition efficiency were compared each other. The result revealed that the recognition rate was much high between normal and disease images, but relatively low between two disease images. In order to increase the recognition efficiency among chest diseases the related algorithms have to be developed continuously in the future study, and such effort will establish the resolute base for CAD.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.2
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• pp.119-124
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• 2005

This study was conducted from March 21 to July 8 in 2004 at JeJu Island to investigate the influences of split nitrogen application times on creeping bentgrass vegetation. Nitrogen rate was 20 kg/10a and it was applied from once to 5 times. The result obtained were summarized as follows; plant height was getting longer as nitrogen was split applied from once to 5 times. So it was longest at 5 times split nitrogen application, but it was no significance from 4 to 5 times. Root length, Minolta SPAD-502 chlorophyll meter reading value, leave and root weight n[e directly proportional plant height response. Degree of land cover Iud density of creeping bentgrass increased as nitrogen was split applied from once to 5 times. But degree of land cover md density of reed decreased. The number of reed species on decreased as nitrogen was split applied from once to 5 times. Then ranking of the dominant weeds were Portulaca oleracea Polygonum hydropiper and Stellaria media (at once split time nitrogen application), Portulaca oleracea, Polygonum hydropiper and Digitaria adscendens (at 2 split times nitrogen application), Digitaria adscendens and Portulaca oleracea Polygonum hydropiper, (at 3 split times nitrogen application), Portulaca oleracea, Poa annua and Polygonum hydropiper (at 4 md 5 split times nitrogen application). These results indicate that the optimum frequency of split N applications is four times for growth of creeping bentgrass in volcanic ash soils of Jeju island.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.9
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• pp.5683-5692
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• 2014

This study examined the correlation among the Korean language ability, health promotion behaviors and health status of immigrant women by marriage. Data on the subjects of 148 immigrant women were collected from September to December, 2013. Constructed self-administered questionnaires were used to gather information on the general characteristics of the immigrant women, their Korean language ability, health promotion behaviors and emotional health status. The health examination results of the subjects were used for information on their health status. There were significant differences in the Korean language reading ability, drinking habits, total cholesterol and body mass index between the diseased group and the intact group. An analysis of the relationship between the Korean language ability and health conditions revealed a significant positive correlation between the total cholesterol and neutral fat, but negative correlations between the density of hemoglobin and the total cholesterol, the neutral fat and body mass index. There was a significant negative correlation between the Korean language ability and the level of stress. Based on these results, a program was suggested and developed to decrease the rate of drinking, cholesterol and body mass index of immigrant women and to promote their ability to understand health information and express their diseases properly in the Korean language.

• Journal of Korean Library and Information Science Society
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• v.51 no.2
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• pp.355-379
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• 2020

Digital Library System(DLS) for the school library is a key access tool for school library materials. The purpose of this study was to find ways to improve the accessibility of materials through analysis of students' information search behavior in DLS. Data were collected through recording of 42 participants' DLS search process, and questionnaire. As a result, the search success rate and search satisfaction were found to be lower when the main purpose of DLS is simple leisure reading, information needs are relatively ambiguous, and when user experiences the complicated situations in the search process. The satisfaction level of search time sufficiency was the highest, and the search result satisfaction was the lowest. Besides, there was a need to improve DLS, such as integrated search of other library collection information, the recommendation of related materials, the print output of collection location, voice recognition through mobile apps, and automatic correction of search errors. Through this, the following can be suggested. First, DLS should complement the function of providing career information by reflecting the demand of education consumers. Second, improvements to DLS functionality to the general information retrieval system level must be made. Third, an infrastructure must be established for close cooperation between school library field personnel and DLS management authorities.

• The Journal of the Korea institute of electronic communication sciences
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• v.6 no.2
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• pp.243-248
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• 2011

In this paper, we propose a new method to extract palm lines and read it with simple techniques from one photo. We use morphological information and 8-directional contour tracking algorithm. From the digitalized image, we transform original RGB information to YCbCr color model which is less sensitive to the brightness information. The palm region is extracted by simple threshold as Y:65~255, Cb:25~255, Cr:130~255 of skin color. Noise removal process is then followed with morphological information of the palm such that the palm area has more than quarter of the pixels and the rate of width vs height is more than 2:1 and 8-directional contour tracking algorithm. Then, the stretching algorithm and Sobel mask are applied to extract edges. Another morphological information that the meaningful edges(palm lines) have between 10 and 20 pixels is used to exclude noise edges and boundary lines of the hand from block binarized image. Main palm lines are extracted then by labeling method. This algorithm is quite effective even reading the palm from a photographed by a mobile phone, which suggests that this method could be used in various applications.

• ALGAE
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• v.32 no.4
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• pp.359-377
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• 2017

Pyropia haitanensis (T. J. Chang et B. F. Zheng) N. Kikuchi et M. Miyata is one of the most commercially useful macroalgae cultivated in southeastern China. In red algae, the biosynthesis of terpenoids through 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway can produce a direct influence on the synthesis of many biologically important metabolites. In this study, two genes of cDNAs, 1-deoxy-D-xylulose-5-phosphate synthase (DXS) and 1-deoxy-D-xylulose-5-phosphate reductase (DXR), which encoding the first two rate-limiting enzymes among MEP pathway were cloned from P. haitanensis. The cDNAs of P. haitanensis DXS (PhDXS) and DXR (PhDXR) both contained complete open reading frames encoding polypeptides of 764 and 426 amino acids residues, separately. The expression analysis showed that PhDXS was significant differently expressed between leafy thallus and conchocelis as PhDXR been non-significant. Additionally, expression of PhDXR and its downstream gene geranylgeranyl diphosphate synthase were both inhibited by fosmidomycin significantly. Meanwhile, we constructed types of phylogenetic trees through different algae and higher plants DXS and DXR encoding amino acid sequences, as a result we found tree clustering consequences basically in line with the "Cavalier-Smith endosymbiotic theory." Whereupon, we speculated that in red algae, there existed only complete MEP pathway to meet needs of terpenoids synthesis for themselves; Terpenoids synthesis of red algae derivatives through mevalonate pathway came from two or more times endosymbiosis of heterotrophic eukaryotic parasitifer. This study demonstrated that PhDXS and PhDXR could play significant roles in terpenoids biosynthesis at molecular levels. Meanwhile, as nuclear genes among MEP pathway, PhDXS and PhDXR could provide a new way of thinking to research the problem of chromalveolata biological evolution.

• Research in Plant Disease
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• v.22 no.4
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• pp.289-292
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• 2016

We surveyed the occurrence of Japanese yam mosaic virus (JYMV) on Yam in Gyeongsangbukdo pronvince from 2013 to 2015. The symptoms of JYMV were yellow stripes and chlorosis in yam leaves and the infection rate was ranged from 33.6% to 40.8%. We determined nucleotide sequence encoding the polyprotein of JYMV isolate BRI from yam leaves using next-generation sequencing (NGS) method. The partial nucleotide portion (7,736 nucleotides) of the genomic RNA of the JYMV isolate BRI has been sequenced (accession No. KU309315). The region sequenced includes a single open reading frame (ORF) encoding a polyprotein composed of 2,497 amino acids containing the coat protein (CP) and 3' untranslated region (UTR). The genomic organization of this isolate shows almost the same to that of other members of JYMV. The JYMV isolate BRI showed 77% to 79% nucleotide identity with the Japanese and Chinese strains and isolates. This is the first report of the genome nucleotide sequence of JYMV from Dioscorea opposita in Korea.