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Diagnosis and Sequence Analysis of Japanese yam mosaic virus from Yam (Dioscorea opposita)

마(Dioscorea opposita)에 발생한 Japanese yam mosaic virus 진단 및 염기서열 분석

  • Lee, Joong-Hwan (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Son, Chang-Gi (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Kwon, Joong-Bae (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Nam, Hyo-Hun (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Kim, Yeong-Tae (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Kim, Mi Kyeong (National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
  • 이중환 (경상북도농업기술원 생물자원연구소) ;
  • 손창기 (경상북도농업기술원 생물자원연구소) ;
  • 권중배 (경상북도농업기술원 생물자원연구소) ;
  • 남효훈 (경상북도농업기술원 생물자원연구소) ;
  • 김영태 (경상북도농업기술원 생물자원연구소) ;
  • 김미경 (농촌진흥청 국립농업과학원) ;
  • 이수헌 (경북대학교 응용생명과학부)
  • Received : 2016.08.26
  • Accepted : 2016.11.07
  • Published : 2016.12.31

Abstract

We surveyed the occurrence of Japanese yam mosaic virus (JYMV) on Yam in Gyeongsangbukdo pronvince from 2013 to 2015. The symptoms of JYMV were yellow stripes and chlorosis in yam leaves and the infection rate was ranged from 33.6% to 40.8%. We determined nucleotide sequence encoding the polyprotein of JYMV isolate BRI from yam leaves using next-generation sequencing (NGS) method. The partial nucleotide portion (7,736 nucleotides) of the genomic RNA of the JYMV isolate BRI has been sequenced (accession No. KU309315). The region sequenced includes a single open reading frame (ORF) encoding a polyprotein composed of 2,497 amino acids containing the coat protein (CP) and 3' untranslated region (UTR). The genomic organization of this isolate shows almost the same to that of other members of JYMV. The JYMV isolate BRI showed 77% to 79% nucleotide identity with the Japanese and Chinese strains and isolates. This is the first report of the genome nucleotide sequence of JYMV from Dioscorea opposita in Korea.

JYMV에 감염된 마는 잎과 엽맥에 부정형의 황화증상을 나타내었고, 마 주산단지인 경북 안동지역에서 조사한 결과 33.6%-40.8%의 감염률을 나타냈다. 마에 발생하는 JYMV 분리주 BRI 게놈의 polyprotein을 코딩하는 영역에 대한 염기서열을 결정하고 이를 JYMV isolate BRI로 명명하고 GenBank에 KU309315로 유전자 등록을 하였으며, 외피단백질 영역에 대한 유연관계 분석에서 대부분의 일본이나 중국과 분리주와는 다른 유연관계를 보이는데 이것은 분화 과정 중에 나타나는 변이주 가능성이 제시되나 추후 광범위한 조사를 통한 정밀한 분석이 필요하다.

Keywords

References

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