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http://dx.doi.org/10.5423/RPD.2016.22.4.289

Diagnosis and Sequence Analysis of Japanese yam mosaic virus from Yam (Dioscorea opposita)  

Lee, Joong-Hwan (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Son, Chang-Gi (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Kwon, Joong-Bae (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Nam, Hyo-Hun (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Kim, Yeong-Tae (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services)
Kim, Mi Kyeong (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.22, no.4, 2016 , pp. 289-292 More about this Journal
Abstract
We surveyed the occurrence of Japanese yam mosaic virus (JYMV) on Yam in Gyeongsangbukdo pronvince from 2013 to 2015. The symptoms of JYMV were yellow stripes and chlorosis in yam leaves and the infection rate was ranged from 33.6% to 40.8%. We determined nucleotide sequence encoding the polyprotein of JYMV isolate BRI from yam leaves using next-generation sequencing (NGS) method. The partial nucleotide portion (7,736 nucleotides) of the genomic RNA of the JYMV isolate BRI has been sequenced (accession No. KU309315). The region sequenced includes a single open reading frame (ORF) encoding a polyprotein composed of 2,497 amino acids containing the coat protein (CP) and 3' untranslated region (UTR). The genomic organization of this isolate shows almost the same to that of other members of JYMV. The JYMV isolate BRI showed 77% to 79% nucleotide identity with the Japanese and Chinese strains and isolates. This is the first report of the genome nucleotide sequence of JYMV from Dioscorea opposita in Korea.
Keywords
Dioscorea opposita; Japanese yam mosaic virus; Sequence analysis;
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