• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.736-742
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• 2017

Objective: Experiments were conducted to clone the sequence of Wild Argali short palate, lung and nasal epithelium clone 1 (SPLUNC1) cDNA, and to lay the foundation for further study the biological function of Wild Argali SPLUNC1. Methods: The complete sequence of Wild Argali SPLUNC1 cDNA was generated by rapid amplification of cDNA ends. The entire coding sequence was inserted into the pPIC9K vector and expressed in Pichia pastoris (P. pastoris) GS115. The recombinant SPLUNC1 protein was detected by Western blot and purified by $Ni^{2+}$ chelate affinity chromatography. The test of effect of the protein on Mycoplasma ovipneumoniae (MO) was performed with real-time polymerase chain reaction. Results: The Wild Argali SPLUNC1 cDNA was 1,076 bp with an open reading frame of 768 bp, which encoded a 26.49 kDa protein composed of 255 amino acids. Its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8%, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of SPLUNC1 cDNA from Ovis aries (accession no. NP_001288334.1), Capra hircus (accession no. XP_005688516.1), Pantholops hodgsonii (accession no. XP_005979709.1), Bos taurus (accession no. NP_776851.1), Felis catus (accession no. XP_006929910.1), Homo sapiens (accession no. NP_001230122.1), Sus scrofa (accession no. NP_001005727.1), Chinchilla lanigera (accession no. NP_001269294.1), Mus musculus (accession no. NP_035256.2), and Rattus norvegicus (accession no. NP_742028.1), respectively. The recombinant protein corresponded to the expected molecular mass of 25.47 kDa as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was detected in the supernatant of P. pastoris, and it could be purified. The results from the test of inhibition effect of argali recombinant SPLUNC1 protein on MO showed that the product could inhibit MO very well (p<0.01). Conclusion: The amino acid sequence of Wild Argali SPLUNC1 was different from other organisms. The recombinant SPLUNC1 protein has good biological activity.

• Journal of Distribution Science
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• v.14 no.3
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• pp.85-91
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• 2016

Purpose - Prior research has suggested that consumers typically prefer to have a larger number of options. However, preference of assortment size may depend on how depleted resources in consumers' mind are. Reduced capacity for self-regulation by resource depletion makes people rely on more intuitive and less effortful decision processing. When they are mentally depleted, people are likely to focus on the choice difficulty from large assortment, which leads to preference for the small assortment when they make a decision. It could be an important question potentially how being in a depleted mode through effortful self-regulation will influence on the evaluation of assortment size. To answer this questioner, we hypothesized that being engaged in self-regulation, as compared with not being engaged in self-regulation, will influence on the evaluation of product assortment size such as attractiveness, difficulty of choice, and anticipated regret. Research design, data, and methodology - In this study, we first manipulated self-regulatory resource availability using a self-regulation task (i.e., instructing participants to solve Sudoku puzzle vs. to solve diagram cube by filling any diagrams that they prefer into cube instead of number) and asked to indicate the difficulty of the tasks available to them ("How much difficulty did you feel when you complete the task?") Next, participants were asked to imagine that they were planning to buy a laptop at one of the two stores (small assortment: 6 options vs. large assortment: 30 options), both offering good quality of products. After reading the product descriptions, participants were instructed to consider all the information and choose a store that they would like to shop. Finally we measured the choice difficulty, evaluation of product assortments, and anticipated regret on a 7-point scale. We conducted two-way ANOVA in testing the main hypothesis that depleted consumers will show poorer subsequent self-control than non-depleted consumers when they make a decision in large assortment. Results - Compared with non-depleted participants, depleted participants showed the bigger difference from the degree of choice difficulty and product attractiveness between large and small assortments, but the result revealed only a significant interaction effect of resource depletion and assortment size on choice difficulty. Also depleted participants showed the smaller difference from the degree of anticipated regret between large and small assortments than non-depleted participants. Conclusion - Depleted individuals by a prior task are relatively effortless and intuitive form of choosing products so that they try to avoid making effortful trade-offs among choice difficulty such as large assortment, compare with non-depleted individuals. However, for anticipated regret, non-depleted individuals in small assortment anticipate more regret by excluding or at least restricting the possibility of buying attractive items or another kind of potential items than depleted individuals, regardless less choice difficulty in small option. To sum up, it is important to note that individuals are influenced by self-regulatory resources and their self-regulatory conditions contribute to the overall positive or negative impact of product assortment on choice.

• Cartoon and Animation Studies
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• s.22
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• pp.31-45
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• 2011

The paper holds its purpose to analyze the descriptive function and meaning of first person animation which the focalizer, character, and all narrators are indicated as 'I', For the purpose, the following was reviewed; the relation between 'I' as child memorizing the days of childhood as adult and the current 'I' as adult, and the aesthetic effect of experience and sense of the child on the audience reading the narration. The retrospective narrating situation of the adult narrator brings descriptive effect which comes from 'the tension between the experiencing self (self as child) and the narrative self (self as adult). The works focus on the content of child experience through the confession of the adult narrator, but the view of the adult always heading towards 'the present'. That is, the aesthetics contained by the first person narrator is related to endless arousal of the values of hidden and forgotten things. In addition, the descriptive method of child focalizer as 'the subject of experience' brings qualitative change which enables reasoning of the subject as itself, which is free from the view tamed by rational system. Becoming an adult, the lost ability of mimesis brings qualitative change by meeting with the generality of childhood sense. Therefore, it can be known that the meaning the narrator contains in the first person narrator condition of animation links with the degree of aesthetic completion of the work, but also, it is a highly strategic descriptive device which determinately affects even the acceptance of audiences regarding the work.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.631-642
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• 2012

This study was carried out to determine the effect of SCB compost leachate on the growth and yield of rice. Field experiment was conducted on sandy loam soil under the different fertilizer management; 80%, 100%, 130% N level of SCB leachate based on 13kg N/10a of conventional farmer application level and chemical fertilizer as control. The plant height and tiller's number of 80 and 100% N levels of SCB leachate were lower than that of the chemical fertilizer plot. But in the plot of 130% N level of SCB leachate the plant height and tillers was higher than that of chemical fertilizer. And the SPAD reading value of leaf in plot of 130% N level was higher than that of the chemical fertilizer. Rice yield in the 80% and 100% SCB leachate was increased from 12 to 13% that of plot of chemical fertilizer. But rice yield of 130% N-level was decreased 7% compared with chemical fertilizer. Rice quality of the application levels of 80 and 100% SCB leachate levels was significantly better than those of chemical fertilizer and 130% level of SCB leachate. In conclusion, the 100% N application of SCB leachate was improved yield and quality of rice.

• Journal of the Korean Chemical Society
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• v.62 no.5
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• pp.398-411
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• 2018

The purpose of this study is to investigate the effects of research project activities using the science writing heuristic on science inquiry abilities and attitudes toward science in high school students. For this purpose, we conducted the research project activities using the science writing heuristic consisting of questioning, experimental design, observation, argument and evidence, reading, and reflection steps for 73 students of the second year of science core course in high school in Jeonnam. In order to analyze the effects of the program, we surveyed the scientific inquiry ability and attitude toward science, investigated the perception of the research project class applying science writing heuristic, and conducted interviews when there was difficulty in interpreting the results. And the results of this study are as follows. First, among the science inquiry abilities, the score of Reasoning, Hypothesis setting, Finding variables, Operational definition, Experimental design, Graphing and data interpretation, Generalization was significantly improved statistically (p<.05), but the score of Expectation was not statistically significant (p>.05). Second, among the attitudes toward science, the score of 'Leisure interest in science', 'Enjoyment of science lessons', 'Career interest in science' was significantly improved statistically (p<.05). And the score of 'Attitude to scientific inquiry' decreased but it's not significant statistically. The high school research project applying science writing heuristic had a positive effect on scientific inquiry ability and scientific attitude but it could be burden to students because it is led by students in a form different from general science class for a long time. And so continuous study on research project that minimize these disadvantages and maximize their merits is needed.

• 한국노년학
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• v.36 no.4
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• pp.1109-1124
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• 2016

We examined the effect of stereotype threat (STT) on spatial working memory and facial emotion recognition in Korean elderly. In addition, we investigated the role of expected moderator such as self-perception of aging. Seventeen seniors (male=7) received basic cognitive tests including K-WMS-IV, MMSE and answered self-report questionnaires including self-perception of aging, anxiety of aging, attitude toward aging and age identity on the first visit. On the second visit, they were exposed to negative stereotype by reading a script detailing cognitive decline related to aging while a control group was exposed to a neutral content. Following the exposure, they were tested on a spatial-working memory task (Corsi-block tapping task) and emotion recognition task (facial expression identification task). The results showed that the seniors exposed to STT showed significantly lower performance on emotion recognition task (p < .05) (i.e., especially on the more difficult facial stimuli). In addition, there was a significant interaction between STT and self-perception of aging (p< .05), indicating that those who have positive self-perception of aging did not show impairment in emotion recognition task and difficult spatial working memory task under STT. On the other hand, those with negative self-perception of aging showed impaired performance under STT. Taken together, the current study suggests that being exposed to STT could negatively influence cognitive and emotional functioning of elderly. Interestingly, having a positive self-perception of aging could protect the underperformance caused by STT.

• The Journal of the Convergence on Culture Technology
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• v.8 no.2
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• pp.235-242
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• 2022

The purpose of this study is to propose an effective class of College English, one of the essential liberal arts subjects of S University, and to analyze the learning effect. This subject targets students with different majors in the first grade, and the learning goal is to improve their reading skills by analyzing students' English skills, especially sentences, as grammatical elements in everyday situations. This thesis examines how College English classes can be conducted in the form of convergence class in the COVID-19 era to induce students' learning motivation and create learning effects. The form of this convergence class is as follows. First, lecture videos, second, Webex real-time non-face-to-face classes, and third, face-to-face classes. In this study, the class procedure of the first class among the classes that were actually conducted during the semester was presented as an example. The researcher specifically surveyed the pre-class questionnaire and conducted the class by grasping the students' English skills and characteristics of the learners through the results. And after taking the course, the questionnaire was surveyed into 30 items and the results were analyzed. Specifically, the results of satisfaction with the composition of the lecture, satisfaction with the lecture video, satisfaction with the face-to-face class, interaction with students, and learning effects were analyzed. This class proposal is a learner-centered model in the form of convergence.

• The Korean Journal of Elementary Counseling
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• v.11 no.1
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• pp.71-91
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• 2012

The purpose of this study is investigate what effects of counseling program using children's poem on maladapted children in school on the children's self-esteem and school adjustment. For this, counseling programs using children's poem was developed and this counseling sessions were implemented to maladapted children in school and the research question to analyze the effect is as follows. First, what effect does the counseling program using children's poem have on the improvement of self-esteem of the maladapted children in school? Secondly, what effect does the counseling program using children's poem have on the school life of the maladapted children in school? The research target were maladapted children in school who are enrolled in the 4th grade in Elementary school. Through two different case studies, these students were selected after reflecting the opinions of the students' current homeroom teacher, parents, and friends. The counseling program using children's poem were conducted from June 2010 to July 2010, twice a week for a total number of 12 sessions. Each session consisted of 40 minutes and client's change process was explored. Through the counseling program using children's poem, this study collected data and examined the effectiveness of the program through comparing the results of the evaluated results of the language and behavior the target children responded, depth interviews with parents, friends, and teachers, behavior observation checklist, and the pre and post test results of the measurement tools. The results of this study is as follows. First, the counseling program using children's poem improved the self-esteem of the maladjusted child in school. Especially, among the sub-region of self-esteem, it was able to improve the domestic, social and academic self-esteem. Secondly, the counseling program using children's poem had a positive effect on the school adjustment of the maladjusted child in school. Among the sub-region of school adjustment, it had a positive change in the teacher relationships, peer relationships, and attitude. In addition, children's poem which was the reading material for the counseling program using children's poem was proven to work effectively in the counseling progress. Recitation and content of the children's poem promoted self-open of the client and as a result, it had a positive effect on improving relations with the counselor and this became the media that led to a successful counseling session. Therefore, I anticipate that this counseling program using children's poem will be actively used in counseling sessions through applying the results of this study and after revising the program so the counseling program could have a more efficient effect on self-esteem and school adjustment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.2
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• pp.253-266
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• 2010

This study describes the prevalence of nutrition labelling use and its association with demographic factors, knowledge and perception of nutrition labelling, and awareness of nutrition labelling usefulness among 282 college students, aged 21~23 years, in Busan Gyeongnam area. The subjects consisted of 150 of male (53.2%) and 132 of female (46.8%) students. 31.3% of male students and 50% of female students reported reading nutrition labelling always or usually, while 34.7% of male and 13.6% of female subjects reported that they don't use nutrition labelling. Although most of the subjects perceived the need of nutrition labelling, their knowledge and understanding of nutrition labelling and their awareness of nutrition labelling usefulness were not enough to apply the information from the nutrition labelling in their daily life, even in the subjects who reported reading nutrition labelling always or usually. This statement might be one of the reasons why there were no significant differences among the groups divided by nutrition labelling use in regards to the dietary habit or snack eating habit in this study. Therefore, a practical nutrition education program on nutrition labelling should be provided for the college students to increase nutrition labelling use and to apply the information from nutrition labelling in their daily life in order to maintain healthy dietary habit.

• Journal of Korean Ophthalmic Optics Society
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• v.17 no.3
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• pp.265-272
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• 2012

Purpose: To compare the results on myopia correction with reverse geometry lenses, effects of wearing reverse geometry lenses were evaluated for the children with low-level and high-level myopia. Methods: The research investigated the corrective effects of having worn reverse geometry lenses for one week, one month, three months and six months on a total of thirty-six persons (sixty-two eyes) between the ages of seven and fifteen, divided into three groups by the degree of their myopia; nineteen eyes(Group One) with myopia of -2.00 D and under, twenty-eight eyes(Group Two) with myopia between -2.25 D and -4 D, and fifteen eyes(Group Three) with myopia of -4.25 D and above; as shown by changes in uncorrected vision and the degree of refraction in the corneal topography, and tested for statistical similarity among the pursued results. Results: After wearing reverse geometry lenses, Group One showed an improvement in vision of 0.5, from 0.45 to 0.95, after one week, and improvements to 0.91 after one month and 1.02 after three months but, after six months, the group's vision regressed to 0.95. Group Two showed an improvement in vision of 0.43, from 0.34 to 0.77, after one week of wearing and to 0.91 after one month, to 0.97 after three months and this was statistically maintained through the remainder of six months. Group Three showed an improvement in vision of 0.55, from 0.15 to 0.7, after wearing for one week, to 0.87 after one month and to 0.91 after three months but saw a regression to 0.86 after six months. The average Sim K (simulated keratometry reading) value for Group One started from $42.84{\pm}1.17D$ and decreased to $41.48{\pm}0.98D$ after one week of wearing and continued declining through three months before increasing during the remainder of six months. Group Two began from $42.91{\pm}1.57D$ and recorded $41.78{\pm}1.58 D$ after one week, continuing the decline through three months before increasing during the remainder of six months. Group Three began at $42.64{\pm}1.64D$ and showed its Sim K value decrease to $40.77{\pm}1.20D$ after one week of wearing, increase after one month and decrease after three months and continue the decline through the remainder of six months. Conclusions: From the results of this study, wearing reverse geometry lenses had myopia-correcting effects after one week of wearing. Although there were variations in the time for such effect to take place but myopia-correcting effects were evident in all test groups.