• 제목/요약/키워드: rapid preparation

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Multi-Residue Analysis of Fipronil and Its Metabolites in Eggs by SinChERS-Based UHPLC-MS/MS

  • Han, Keguang;Hua, Jin;Zhang, Qi;Gao, Yuanhui;Liu, Xiaolin;Cao, Jing;Huo, Nairui
    • 한국축산식품학회지
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    • 제41권1호
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    • pp.59-70
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    • 2021
  • A method for simultaneous detection of fipronil (F) and its metabolites fipronil desulfinyl (FD), fipronil sulfide (FS), fipronil sulfone (FSO) in chicken eggs was applied and validated. It includes single-step, cheap, effective, rugged, safe-based method (SinChERS) for sample preparation and ultra high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) for chemical analysis. Results suggested that formic acid enhanced the recovery of 4 target residues and 1% supplementation to acetonitrile gained higher recoveries than that of 5%. SinChERS integrated extraction and clean-up steps into one, with shorter time (1.5 h) to operate and higher recoveries (97%-100%) than HLB, Envi-Carb-NH2 and quik-easy-cheap-effective-rugged-safe method (QuEChERS), and it consumed the smallest volume of extracting solvent (10 mL) as QuEChERS. Quantitative analyses using external standard method suggested the linear ranges of 4 target compounds were 1-20 ㎍/L with R2 >0.9947. The limit of detection (S/N>3) and quantification (S/N>10) were 0.3 ㎍/kg and 1 ㎍/kg. Recoveries ranged from 89.0% to 104.4%, and the relative standard deviations (n=6) at 1, 10, and 20 ㎍/kg were lower than 6.03%. Thirty batches of domestic eggs (500 g each) were detected by the established SinChERS-based UHPLC-MS/MS and no target residues were detected in all samples. The method developed in this study is a rapid, sensitive, accurate and economic way for multi-residue analysis of fipronil and its metabolites in eggs.

코발트 페라이트 나노입자/탄소 나노섬유 복합전극 제조 및 슈퍼커패시터 특성평가 (Preparation of CoFe2O4 Nanoparticle Decorated on Electrospun Carbon Nanofiber Composite Electrodes for Supercapacitors)

  • 황혜원;육서연;정민식;이동주
    • 한국분말재료학회지
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    • 제28권6호
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    • pp.470-477
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    • 2021
  • Energy storage systems should address issues such as power fluctuations and rapid charge-discharge; to meet this requirement, CoFe2O4 (CFO) spinel nanoparticles with a suitable electrical conductivity and various redox states are synthesized and used as electrode materials for supercapacitors. In particular, CFO electrodes combined with carbon nanofibers (CNFs) can provide long-term cycling stability by fabricating binder-free three-dimensional electrodes. In this study, CFO-decorated CNFs are prepared by electrospinning and a low-cost hydrothermal method. The effects of heat treatment, such as the activation of CNFs (ACNFs) and calcination of CFO-decorated CNFs (C-CFO/ACNFs), are investigated. The C-CFO/ACNF electrode exhibits a high specific capacitance of 142.9 F/g at a scan rate of 5 mV/s and superior rate capability of 77.6% capacitance retention at a high scan rate of 500 mV/s. This electrode also achieves the lowest charge transfer resistance of 0.0063 Ω and excellent cycling stability (93.5% retention after 5,000 cycles) because of the improved ion conductivity by pathway formation and structural stability. The results of our work are expected to open a new route for manufacturing hybrid capacitor electrodes containing the C-CFO/ACNF electrode that can be easily prepared with a low-cost and simple process with enhanced electrochemical performance.

편집자 주 - 30권 3호 (Editorial for Vol. 30, Issue 3)

  • 김영효
    • 항공우주의학회지
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    • 제30권3호
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    • pp.83-85
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    • 2020
  • In commemoration of Vol. 30, Issue 3, our journal prepared five review articles and one original paper. The global outbreak of COVID-19 in 2020 has impacted our society, and especially the aviation and travel industries have been severely damaged. Kwon presented the aviation medical examination regulations related to COVID-19 announced by the Ministry of Land, Infrastructure, and Transport of the Republic of Korea. Lim summarized various efforts of airlines to overcome the crisis in the aviation industry. He also discussed the management of these aircraft as the number of airplanes landing for long periods increased. Finally, he suggested various quarantine guidelines at airports and onboard aircraft. COVID-19 has had a profound impact on mental health as well as physical effects. Kim investigated the impact of COVID-19 on mental health and suggested ways to manage the stress caused by it. The Internet of Things (IoT) refers to a technology in which devices communicate with each other through wired or wireless communication. Hyun explained the current state of the technology of the IoT and how it could be used, especially in the aviation field. In the area of airline service, various situations arise between passengers and crew. Therefore, role-playing is useful in performing education to prepare and respond to passengers' different needs appropriately. Ra introduced the conceptual background and general concepts of role-playing and presented the actual role-play's preparation process, implementation, evaluation, and feedback process. For a fighter to fly for a long time and perform a rapid air attack, air refueling is essential, which serves refueling from the air rather than from the aircraft base. Koo developed a questionnaire based on the HFACS (Human Factors Analysis and Classification System) model and used it to conduct a fighter pilot survey and analyze the results.

만성질환 관리를 위한 PDA폰 기반 이동형 방문건강관리 시스템 개발 (A Study on a PDA-Based Ubiquitous Healthcare System for the Management of Chronic Diseases)

  • 손성용;황원숙;이양희;김창섭;임채승;박길홍
    • 가정간호학회지
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    • 제14권1호
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    • pp.18-22
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    • 2007
  • Purpose: The rapid change to an aging society generates an increase in the incidence of chronic diseases. Many chronic patients have been facing their illnesses without enough preparation. In order to solve these problems, we designed and tested a public healthcare service based on ubiquitous technology. Method: Telemedicine has emerged as new medical care system of chronic disease. However, public potential of its technology is difficult to know under current traditional health care system. In this work, we developed a Personal Digital Access (PDA) phone based healthcare system by trained visiting nurses for elderly patients. A field test was performed by SeongBuk Public Health Center in Seoul, Republic of Korea (ROK). Result: Surveys were generated to assess the effects of this system compared to conventional public health system. Findings of trials demonstrate that healthcare coordination enhanced by PDA phone technology is satisfactory to the patients and nurses compared to previous one because prompt responses arouse their recognition of health. Conclusion: Ubiquitous healthcare system applied to public health service proved to be efficient and time-saving to monitor and control the chronic illnesses of large population.

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SNA 분석을 이용한 해상 수출입화물의 네트워크 구조와 국내 항만의 중심성 분석 (An Analysis on the Centrality of Domestic Areas and Ports: Using SNA Methodology)

  • 김주혜;김치열
    • 한국항만경제학회지
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    • 제38권4호
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    • pp.25-43
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    • 2022
  • 과거와 달리 물류를 공간적, 서비스 영역의 확장에 따라 네트워크 관점으로 해석해야하는 노력이 필요하다. 또한 다양한 환경의 변화로 물류 네트워크 역시 급변하고 있다. 따라서 본 연구는 사회네트워크 분석을 이용하여 해상 수출입화물의 구조와 국내 항만의 중심성의 변화 추이를 분석하는데 목적을 두었다. 2005~2020년 기간에 5년 간격의 국내 해상 수출입화물의 전체 교역데이터를 사용하여, 우리나라 해상 수출입화물의 네트워크 구조의 변화를 알아보았으며, 국내 항만의 중심성에 영향을 끼치는 주요 요인을 알 수 있었다. 분석결과로는 연결 중심성은 울산항이 가장 높으며, 내향 근접 중심성은 부산, 인천항, 외향 근접 중심성은 부산, 울산항 순으로 분석되었다. 중개자 역할의 항만이라고 볼 수 있는 매개 중심성이 가장 높은 항만은 부산, 울산항 순으로 나타났다. 본 연구는 항만의 중심성 지표의 변화와 해상화물 네트워크 파악을 토대로 20년간의 국내 수출입화물 교역에 관련한 일련의 패턴을 예측하고 리스크를 대비하는 참고자료로 활용될 수 있을 것이다.

Antibody radiolabeling with diagnostic Cu-64 and therapeutic Lu-177 radiometal

  • Abhinav Bhise;Jeongsoo Yoo
    • 대한방사성의약품학회지
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    • 제8권1호
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    • pp.45-49
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    • 2022
  • With the development of monoclonal antibodies, therapeutic or diagnostic radioisotope has been successfully delivered at tumor sites with high selectivity for antigens. Different approaches have been applied to improve the tumor-to-normal ratio by considering the in vivo stability of radioimmunoconjugates as a prerequisite. Various stable and inert antibody radiolabeling techniques for radioimmunoconjugate preparation have been extensively evaluated to enhance in vivo stability. Antibody radiolabeling techniques should be rapid and easy; they should not disrupt the immunoreactivity and in vivo behavior of antibodies, which are coupled with a bifunctional chelator (BFC) to stably coordinate with a radiometal. For the design of BFCs, radiometal coordination properties must be considered. However, various diagnostic radionuclides, such as 89Zr, 64Cu, 68Ga, 111ln, and 99mTc, or therapeutic radionuclides, such as 177Lu, 67Cu, 90Y, and 225Ac, have been increasingly used for antibody radiolabeling. In addition to useful radionuclides, 64Cu and 177Lu with the most accessible or the highest production rates in many countries should be considered. In this review, we mainly discussed antibody radiolabeling techniques and conditions that involve 64Cu and 177Lu radiometals.

실시간 지진데이터 품질향상을 위한 연구 (A Study on the Quality Improvement of Real-Time Earthquake Data)

  • 윤진섭;류세환;정지은;박지민
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2022년도 춘계학술대회
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    • pp.306-309
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    • 2022
  • 최근 우리나라에서도 지진 발생빈도가 급격히 증가하면서 지진에 대한 관심도가 높아지고 있다. 지진발생 시 대응과 복구도 중요하지만, 관리 취약점을 사전에 찾아내어 예방 및 사전 대비 활동을 수행하는 것이 필요하다. 한국수자원공사에서는 지진으로부터 안전한 시설관리를 위해 지진가속도계측기를 운영하여 실시간 지진데이터를 수집하고 있으며, 대외기관에서 지진데이터를 활용할 수 있도록 데이터를 전송하고 있다. 운영 중인 지진감시시스템의 관리 취약점을 사전에 줄이고자 지진데이터의 수집지연시간을 최소화하고, 빅데이터를 활용한 지진데이터의 실시간 품질분석 체계·기반 구축으로 고품질의 데이터 생산이 가능하다. 이에 따라 댐 안전관리 및 고품질의 지진데이터를 확보하여 대외기관에 신속한 데이터를 제공함으로써 지진재해로부터 국민의 생명과 재산을 보호할 수 있을 것으로 기대된다.

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역상 고성능 액체 크로마토그래피를 이용한 마늘에서 diallyl disulfide의 분석 (Determination of diallyl disulfide in garlic by reversed-phase high performance liquid chromatography)

  • 만효룡;;노경호
    • 분석과학
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    • 제20권5호
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    • pp.442-447
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    • 2007
  • 마늘(Allium sativum L.)의 특성은 organosulfur 화합물이다. 마늘에 포함된 diallyl disulfide(DADS)를 분석하기 위한, 간단하고 신속한 시료준비와 분석방법을 제시하였다. 모든 마늘시료들은 메탄올, 벤젠, 또는 테트라하이드로퓨란과 같은 용매로 추출하여 분석하였다. 실험결과에 의하면, 메탄올로 추출한 시료가 가장 우수하였다. 분석조건으로는 이동상은 메탄올과 물로 구성되고 기울기법을 적용하였다. 분말가루로 된 마늘 1 g에 0.61 mg DADS를 추출하였다. 기존 분석방법에 비해 우수하여 마늘관련 연구에 도움이 될 것이다.

무기산 누출 사고 대응을 위한 탐지·분석 방법 연구 (Study on the Methods of Detection and Analysis for Responding Inorganic Acids Spill)

  • 이진선;정미숙;김기준;안성용;윤영삼;윤준헌
    • 한국위험물학회지
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    • 제2권1호
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    • pp.6-11
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    • 2014
  • There have been frequent chemical leaks over the past 10 years. Particularly, inorganic acids like sulfuric acid, nitric acid, and hydrogen chloride take up 37 % of the total chemical accidents which took place for the past 10 years. When an acid chemical leak happens, fume is generated, diffusing into the air, which might cause serious damage to health of local residents and the environment. However, most of the acid-based chemicals, detecting and analysis methods have not been settled considering the frequency of accidents. In this study, we investigated detection and analysis methods to quickly analyze accident sites and evaluate the impacts on environments. Reviewing local and international test analysis methods of acids suggested that nitric acid, sulfuric acid, hydrogen chloride and hydrogen fluoride can be analyzed with IC. It was also found that UV is better for the analysis of hydrogen fluoride and GC/MS for acrylic acid. The analytical methods suggested in the official test methods basically have limitations of consuming much time at stages of preparation and analysis. Considering prompt responses to chemical accidents, further studies should be done to compare the applicability of rapid monitoring methods such as FT-IR, IMR-MS and SIFT-MS.

Copper Oxide Spike Grids for Enhanced Solution Transfer in Cryogenic Electron Microscopy

  • Dukwon Lee;Hansol, Lee;Jinwook Lee;Soung-Hun Roh;Nam-Chul Ha
    • Molecules and Cells
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    • 제46권9호
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    • pp.538-544
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    • 2023
  • The formation of uniform vitreous ice is a crucial step in the preparation of samples for cryogenic electron microscopy (cryo-EM). Despite the rapid technological progress in EM, controlling the thickness of vitreous ice on sample grids with reproducibility remains a major obstacle to obtaining high-quality data in cryo-EM imaging. The commonly employed classical blotting process faces the problem of excess water that cannot be absorbed by the filter paper, resulting in the formation of thick and heterogeneous ice. In this study, we propose a novel approach that combines the recently developed nanowire self-wicking technique with the classical blotting method to effectively control the thickness and homogeneity of vitrified ice. With simple procedures, we generated a copper oxide spike (COS) grid by inducing COSs on commercially available copper grids, which can effectively remove excess water during the blotting procedure without damaging the holey carbon membrane. The ice thickness could be controlled with good reproducibility compared to non-oxidized grids. Incorporated into other EM techniques, our new modification method is an effective option for obtaining high-quality data during cryo-EM imaging.