• Title/Summary/Keyword: putrescine

Search Result 177, Processing Time 0.023 seconds

Effects of Polyamines and Methylglyoxal bis(guanylhydrazone) on Activity of Diamine Oxidase in Soybean (Glycine max) Seedlings without Cotyledons (자엽을 제거한 대두 유식물에서 Polyamine과 Methylglyoxal bis(guanylhydrazone)가 Diamine Oxidase의 활성에 미치는 영향)

  • 강정훈
    • Journal of Plant Biology
    • /
    • v.34 no.1
    • /
    • pp.53-57
    • /
    • 1991
  • The effect of polyamines and methylglyoxal bis(guanylhydrazone)(MGBG) on the activity of diamine oxidase was studied in soybean (Glycine max) seedlings. $10^{-2}\;M$ of putrescine, spennidine, and spermine inhibited diamine oxidase activity, whereas $10^{-6}\;M$ putrescine increased enzyme activity. These results suggest that diamine oxidase can be induced by a specific substrate, putrescine. The content of putrescine was increased in response to the increase in concentratioin of MGBG. In vitro, 40% of the diamine oxidase activity was inhibited by $10^{-3}\;M$ MGBG. In vivo, the diamine oxidase activity was increased by a low concentrration of MGBG. It was suggested that MGBG inhibited the formation of spermidine and that the accumulated putrescine induced diamine oxidase, whereas the diamine oxidase acitivity was inhibited by a high concentration of MGBG. It is suggested that a high cocentration of MGBG increases the putrescine content by inhibiting diamine oxidase activity which is responsible for putrescine degradation.dation.

  • PDF

Effects of Putrescine on Senescence in Detached Leaves of Chinese Cabbage in the Light (광조건에서 Putrescine이 잘라낸 배추잎의 노쇠과정에 미치는 효과)

  • 조형택
    • Journal of Plant Biology
    • /
    • v.31 no.3
    • /
    • pp.227-237
    • /
    • 1988
  • Effects of putrescine on senescence in detached leaves of Chinese cabbage (Brassica campestris L.) in the light were investigated. The putrescine as a potent antisenescence substane markedly inhibited chlorophyll and protein loss at the 10mM concentraton in the detached leaves during the dark incubation. In the light, however, putrescine showed the opposite effects to dark incubation. The chlorphyll loss by putrescine in the light was stopped with darktransfer, and inhibited competitively by a divalent cation Ca2+. In the light, putrescine reduced the protease activity. Putrescine, in the light, increased H2O2 content and reduced the activities of enzymes -superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6-involved in inhibiting the accumulation of free radicals. These results suggest that the effects of puterscine on chlorophyll and protein loss in detached leaves of Chinese cabbage in the light are related to the cationic nature of putrescine and the accumulation of free radicals.

  • PDF

A Study of Polyamine Biosynthetic Enzymes and Content of Polyamine in Ginseng (인삼에서의 Polyamine 합성에 관련된 효소와 Polyamine 함량에 관한 연구)

  • 조병구;조영동
    • Journal of Ginseng Research
    • /
    • v.13 no.1
    • /
    • pp.19-23
    • /
    • 1989
  • The polyamine and its biosynthetic enzyme In ginseng has been studied , We have found that putrescine was a major polyamine in ginseng seedling. Putrescine was increased with growth stage , and ADC activity was also increased as putnescine content. But ODC activity was not shown. ADC activity was shown l0% inhibition by spermidine and 20% inhibition by spermine but not affected by putrescine. in contrast to seed, spermidine was a major polyamine in two year old ginseng. The abundance was decreased in order of leaf, petiole, root, and stem. ADC activity seemed to parallel with polyamine content.

  • PDF

Putrescine Transport in a Cyanobacterium Synechocystis sp. PCC 6803

  • Raksajit, Wuttinun;Maenpaa, Pirkko;Incharoensakdi, Aran
    • BMB Reports
    • /
    • v.39 no.4
    • /
    • pp.394-399
    • /
    • 2006
  • The transport of putrescine into a moderately salt tolerant cyanobacterium Synechocystis sp. PCC 6803 was characterized by measuring the uptake of radioactively-labeled putrescine. Putrescine transport showed saturation kinetics with an apparent $K_m$ of $92{\pm}10\;{\mu}M$ and $V_{max}$ of $0.33{\pm}0.05\;nmol/min/mg$ protein. The transport of putrescine was pH-dependent with highest activity at pH 7.0. Strong inhibition of putrescine transport was caused by spermine and spermidine whereas only slight inhibition was observed by the addition of various amino acids. These results suggest that the transport system in Synechocystis sp. PCC 6803 is highly specific for polyamines. Putrescine transport is energy-dependent as evidenced by the inhibition by various metabolic inhibitors and ionophores. Slow growth was observed in cells grown under salt stress. Addition of low concentration of putrescine could restore growth almost to the level observed in the absence of salt stress. Upshift of the external osmolality generated by either NaCl or sorbitol caused an increased putrescine transport with an optimum 2-fold increase at 20 mosmol/kg. The stimulation of putrescine transport mediated by osmotic upshift was abolished in chloramphenicol-treated cells, suggesting possible involvement of an inducible transport system.

Isolation and Characterization of Putrescine-producing Bacteria in Commercially Available Sauces Made from Salted and Fermented Sand Lance Ammodytes personatus (시판 까나리(Ammodytes personatus) 액젓에서 Putrescine 생성균의 분리 및 특성)

  • Um, In-Seon;Kim, Tae-Ok;Park, Kwon-Sam
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.49 no.5
    • /
    • pp.573-581
    • /
    • 2016
  • Bacterial decarboxylation of amino acids in food leads to the production of biogenic amines, which can cause reactions in human that include headaches, nausea, palpitations, chills, and severe respiratory distress. The amine putrescine is an especially effective inhibitor of metabolizing enzymes and amplifies histamine intoxication and tyramine poisoning. Using an L-ornithine decarboxylating medium, we isolated 14 putrescine-producing bacteria from sand lance, Ammodytes personatus, sauces. The isolates were identified, using an API kit and 16S rRNA analysis, as Lysinibacillus fusiformis (1 strain), Lysinibacillus xylanilyticus (6 strains), Lysinibacillus macroides (1 strain), Lysinibacillus sphaericus (3 strains), Bacillus fusiformis (1 strain), Paenibacillus favisporus (1 strain), and Staphylococcus caprae (1 strain). These strains produced between 1.66 to 236.97 μg/mL of putrescine after 48 h incubation. Lysinibacillus spp. were the dominant putrescine-producing bacteria in sand lance sauces, which produced 236.97 μg/mL of putrescine from a culture broth containing 0.5% L-ornithine. This is the first report on the isolation and identification of putrescine-producing bacteria from sand lance sauces.

Validation and Content Analysis of Putrescine in the Venom of Honeybee (Apis mellifera L.) (서양종꿀벌 일벌독에 함유된 putrescine 밸리데이션 및 함량 분석)

  • Choi, Hong Min;Kim, Hyo Young;Kim, Se Gun;Han, Sang Mi
    • Korean journal of applied entomology
    • /
    • v.60 no.3
    • /
    • pp.263-268
    • /
    • 2021
  • The venom of honeybees (Apis mellifera L.) is used to treat many diseases because of its anti-inflammatory and analgesic effects. Bee venom consists of several biologically active molecules and exhibits remarkable anti-cancer effects. However, biological amines, which exhibit diverse functionality such as anti-inflammatory and antibacterial effects, have not been previously reported in bee venom. In this study, we determined the content of putrescine in bee venom by using ultra-performance liquid chromatography. The specificity, accuracy, and precision of the assay were assessed, and the assay validated. The linearity of the putrescine assay was r ≥ 0.99, indicating a moderate level of putrescine in the bee venom. The limit of detection and limit of quantification were both 0.9 ㎍/mL, while the rate of recovery was 96.4%-99.9%. The relative standard deviation (RSD) of the intra-day precision and inter-day precision of the putrescine assay were 0.16% - 0.23% and 0.09% - 0.36%, respectively, with the RSD ≤ 5% indicating excellent precision. Thus, the linearity, limit of detection, limit of quantification, and recovery rate of the putrescine assay were satisfactory. The analysis of the bee venom showed that the putrescine content was 3.1 ± 0.09 mg/g. This study provides fundamental data on putrescine content in bee venom, which will prove useful in further studies of its bioactivity.

Validation of UPLC Analysis Method for Putrescine in Lyophilized Royal Jelly (동결건조 로열젤리 내 putrescine 함량 분석을 위한 분석법 밸리데이션)

  • Hong-Min, Choi;Se-Gun, Kim;Hyo-Young, Kim;Soon-Ok, Woo;Sang-Mi, Han
    • Korean journal of applied entomology
    • /
    • v.61 no.4
    • /
    • pp.659-664
    • /
    • 2022
  • Putrescine generated by the action of microorganisms in the decay generally used as a measure of freshment in food. However, the analytical method of putrescine in freeze-dried royal jelly has not yet been established. In the present study, the UPLC method for putrescine in lyophilized royal jelly was established using C18 column. The newly established method was able to analyze putrescine accurately within 7 minutes and was validated by analytical parameters such as specificity, linearity, precision, accuracy, limit of detection, and limit of quantification. These results provide for the analytical method to evaluate the level of freshment in freeze-dried royal jelly, which will useful in further studies of safety verification.

Determination of Putrescine, Spermidine and Spermine in urine by HPLC (HPLC법에 의한 뇨 중의 Putrescine, Spermidine 및 Spermine의 정량)

  • Shin, Tae-Yong;Lee, Seok-Bong;Kim, Hyung-Min
    • Analytical Science and Technology
    • /
    • v.10 no.6
    • /
    • pp.445-452
    • /
    • 1997
  • A simple and sensitive high performance liquid chromatography for analysis of Putrescine, Spermidine and Spermine in urine is described. The benzoyl chloride derivatives of Putrescine, Spermidine and Spermine are separated on a ${\mu}$ Bondapak $C_{18}$ reverse-phase column with methanol/$H_2O$(50/50v/v) as the mobile phase. The results show that the mean level of polyamines in uterine cancer patients urine is much higher than in normal human urine.

  • PDF

Utilization of Putrescine by Streptococcus pneumoniae During Growth in Choline-limited Medium

  • Ware D.;Watt J.;Swiatlo E.
    • Journal of Microbiology
    • /
    • v.43 no.5
    • /
    • pp.398-405
    • /
    • 2005
  • Polyamines such as putrescine are small, ubiquitous polycationic molecules that are required for optimal growth of eukaryotic and prokaryotic cells. These molecules have diverse effects on cell physiology and their intracellular content is regulated by de novo synthesis and uptake from the environment. The studies presented here examined the structure of a putative polyamine transporter (Pot) operon in Streptococcus pneumoniae (pneumococcus) and growth of pneumococci in medium containing putrescine substituted for choline. RT-PCR experiments demonstrated that the four genes encoding the Pot system are co-transcribed with murB, a gene involved in an intermediary step of peptidoglycan synthesis. Pneumococci grown in chemically-defined media (CDM) containing putrescine without choline enter logarithmic phase growth after 36-48 hs. However, culture density at stationary phase eventually reaches that of choline-containing medium. Cells grown in CDM-putrescine formed abnormally elongated chains in which the daughter cells failed to separate and the choline-binding protein PspA was no longer cell-associated. Experiments with CDM containing radiolabeled putrescine demonstrated that pneumococci concentrate this polyamine in cell walls. These data suggest that pneumococci can replicate without choline if putrescine is available and this polyamine may substitute for aminoalcohols in the cell wall teichoic acids.

Association of Dexamethasone-induced Apoptosis and $G_l-Arrest$ of Human Leukemic CEM Cells with Polyamine Deficit

  • Choi, Sang-Hyun;Lee, Jung-Ae;Chae, Yang-Seok;Min, Bon-Hong;Chun, Yeon-Sook;Chun, Boe-Gwun
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.1 no.4
    • /
    • pp.457-466
    • /
    • 1997
  • The effects of DFMO or/and putrescine on the dexamethasone-induced apoptosis of CEM cells were studied to investigate the role of polyamines in anti-leukemic glucocorticoid action. Dexamethasone- induced apoptosis was preceded by significant decreases of cellular polyamine contents and putrescine uptake activity. But DFMO produced decreases of putrescine and spermidine contents and marked increase of putrescine uptake activity, but did not induce apoptosis. However, dexamethasone and DFMO, respectively, induced $G_1-arrest$ in cell cycle and hypophosphorylation of pRb, resulting in the increase of $G_1$ to S ratio and decrease of CEM cell count. DFMO enhanced the dexamethasone-induced apoptosis and $G_1-arrest$. On the other hand, putrescine little affected the apoptotic and $G_1-arresting$ activities of dexamethasone, but almost suppress the effects of DFMO and also the DFMO-dependent enhancement of dexamethasone effects. These results suggested that the dexamethasone-induced apoptosis to be associated with pRb hypophosphorylation and $G_1-arrest$ in CEM cells might be ascribed to the concomitant decreases of cellular polyamine contents and putrescine uptake activity.

  • PDF