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http://dx.doi.org/10.5656/KSAE.2022.11.0.067

Validation of UPLC Analysis Method for Putrescine in Lyophilized Royal Jelly  

Hong-Min, Choi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Se-Gun, Kim (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Hyo-Young, Kim (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Soon-Ok, Woo (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Sang-Mi, Han (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Publication Information
Korean journal of applied entomology / v.61, no.4, 2022 , pp. 659-664 More about this Journal
Abstract
Putrescine generated by the action of microorganisms in the decay generally used as a measure of freshment in food. However, the analytical method of putrescine in freeze-dried royal jelly has not yet been established. In the present study, the UPLC method for putrescine in lyophilized royal jelly was established using C18 column. The newly established method was able to analyze putrescine accurately within 7 minutes and was validated by analytical parameters such as specificity, linearity, precision, accuracy, limit of detection, and limit of quantification. These results provide for the analytical method to evaluate the level of freshment in freeze-dried royal jelly, which will useful in further studies of safety verification.
Keywords
Biogenic amine; Putrescine; Validation; Freeze-dried royal jelly;
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