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http://dx.doi.org/10.5656/KSAE.2020.03.1.071

Validation and Content Analysis of Putrescine in the Venom of Honeybee (Apis mellifera L.)  

Choi, Hong Min (Sericulture and Apiculture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
Kim, Hyo Young (Sericulture and Apiculture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
Kim, Se Gun (Sericulture and Apiculture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
Han, Sang Mi (Sericulture and Apiculture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
Publication Information
Korean journal of applied entomology / v.60, no.3, 2021 , pp. 263-268 More about this Journal
Abstract
The venom of honeybees (Apis mellifera L.) is used to treat many diseases because of its anti-inflammatory and analgesic effects. Bee venom consists of several biologically active molecules and exhibits remarkable anti-cancer effects. However, biological amines, which exhibit diverse functionality such as anti-inflammatory and antibacterial effects, have not been previously reported in bee venom. In this study, we determined the content of putrescine in bee venom by using ultra-performance liquid chromatography. The specificity, accuracy, and precision of the assay were assessed, and the assay validated. The linearity of the putrescine assay was r ≥ 0.99, indicating a moderate level of putrescine in the bee venom. The limit of detection and limit of quantification were both 0.9 ㎍/mL, while the rate of recovery was 96.4%-99.9%. The relative standard deviation (RSD) of the intra-day precision and inter-day precision of the putrescine assay were 0.16% - 0.23% and 0.09% - 0.36%, respectively, with the RSD ≤ 5% indicating excellent precision. Thus, the linearity, limit of detection, limit of quantification, and recovery rate of the putrescine assay were satisfactory. The analysis of the bee venom showed that the putrescine content was 3.1 ± 0.09 mg/g. This study provides fundamental data on putrescine content in bee venom, which will prove useful in further studies of its bioactivity.
Keywords
Apis mellifera L.; bee venom; biological amine; putrescine; validation;
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