• 한국미생물·생명공학회지
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• 제27권2호
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• pp.172-178
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• 1999

In this study, we tested possibility of replacing nitrite salts, which were always added during the meat product processing, with the metabolites produced by antimicrobial and red pigment producing Monascus strains. We have already shown that Monascus No. 116 strain has the highest antimicrobial activity among the strains isolated from Ang-Khak. Monascus isolate No. 229 was chosen due to its outstanding red pigment producing ability. The red pigment production by No. 229 was highest in the medium containing 8% sucrose, 2% yeast extract, 0.1% K2HPO4, 0.5% MgSO4. Optimum pH and temperature for the red pigment production were pH 6.2 and 3$0^{\circ}C$, was found in spot or Rf value 0.54 on TLC plate using ethyl acetate-acetone-water (4:4:1, v/v/v) as development solvent system. Isolate No. 116 and No. 229 were cultured in a optimal condition for the antimicrobial activity and red pigmentation. The culture concentrates were applied in situ to the production of instantly processed ham. Mixed application of 89 ppm Na-nitrite and 300 ppm of culture broth concentrate of Monascus isolate No. 116 and 500 ppm of red color produced by Monascus isolate No. 229 showed similar results with the single application of 94 ppm Na-nitrite. These results confirmed that the antimicrobial activity and red pigment of Monascus strains might be valuable to replace Na-nitrite salt in meat processing.

• Journal of Preventive Medicine and Public Health
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• 제47권5호
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• pp.253-257
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• 2014

Arsenic is a ubiquitous, naturally occurring metalloid that may be a significant risk factor for cancer after exposure to contaminated drinking water, cigarettes, foods, industry, occupational environment, and air. Among the various routes of arsenic exposure, drinking water is the largest source of arsenic poisoning worldwide. Arsenic exposure from ingested foods usually comes from food crops grown in arsenic-contaminated soil and/or irrigated with arsenic-contaminated water. According to a recent World Health Organization report, arsenic from contaminated water can be quickly and easily absorbed and depending on its metabolic form, may adversely affect human health. Recently, the US Food and Drug Administration regulations for metals found in cosmetics to protect consumers against contaminations deemed deleterious to health; some cosmetics were found to contain a variety of chemicals including heavy metals, which are sometimes used as preservatives. Moreover, developing countries tend to have a growing number of industrial factories that unfortunately, harm the environment, especially in cities where industrial and vehicle emissions, as well as household activities, cause serious air pollution. Air is also an important source of arsenic exposure in areas with industrial activity. The presence of arsenic in airborne particulate matter is considered a risk for certain diseases. Taken together, various potential pathways of arsenic exposure seem to affect humans adversely, and future efforts to reduce arsenic exposure caused by environmental factors should be made.

• Journal of the Korean Wood Science and Technology
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• 제38권5호
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• pp.450-456
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• 2010

This research was performed to develop the simple techniques to predict the copper concentration in alkaline copper quat (ACQ), copper azole (CUAZ), and bis-(N-cyclohexyl-diazeniumdioxy)-copper (CB-HDO) solutions. Two simple methods measuring the color due to copper compounds were evaluated by using a spectrophotometer. One is to directly measure the color of the preservative solutions. The other is to measure the color developed on the surface of a treated sample with the preservatives. The $L^*$ of the measured color values appeared to be the most sensitive to the change of copper concentration. The $a^*$ values of the preservative solutions tended to be decreased at above a certain concentration condition, and the $b^*$ values showed no trend with the concentration of copper compounds in preservative solutions. The surface color of the treated samples were changed from bluish to greenish as time passed. Both methods showed the high $R^2$ values of the regression models determined by using the lightness, which suggested that the methods might be applicable in preservative-treatment mills for the easy and fast prediction of the copper concentration.

• 한국식생활문화학회지
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• 제16권4호
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• pp.296-309
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• 2001

This study was conducted to investigate the consumers' recognition and information need for food safety, especially focused on pesticide residues, foodborne illness, and food additives. The data were collected from 350 adults living in Taegu and Pusan by the self-administered questionnaire. Frequencies and chi-square tests were conducted by SPSS. The results of the survey were as follows: (1) the consumers' concerns about food safety were high, (2) many respondents worried about eating vegetables, fruits and grains in turn because of pesticide residues, and did not trust the results from food-safety tests, (3) the major factor for foodborne illness was regarded as unfreshed or contaminated food stuffs, (4) many consumers concerned highly about preservatives among food additives, and tried to consume food containing less food additives, and (5) many respondents wanted to get the information about harmfulness of pesticide residues in foods, method to choose fresh food, and safety of food additives.

• 동아시아식생활학회지
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• 제21권3호
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• pp.401-417
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• 2011

The purpose of this study was to investigate consumer concerns about hazardous elements on foods and recognition of the roles of government, food producers, and consumers in securing food safety. The data were collected from the 443 adult consumers living in Seoul, Busan, Daegu, Incheon, Kwangju and Daejeon through a self-administered questionnaire. Frequencies, t tests, analysis of variance and Duncan's multiple range tests were conducted using SPSS Windows. The results of the survey were as follows: (1) the respondents were worried about health risks related to hazardous elements on foods, (2) many respondents were more worried about preservatives of imported foods than those of domestic foods, and (3) most respondents mentioned that observance of enforcement regulations by producers was the most important factor for ensuring food safety in the future. These results imply that regulations regarding the safety of imported foods should be increased.

• Journal of Microbiology and Biotechnology
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• 제25권4호
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• pp.479-491
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• 2015

Lactobacillus species have been shown to enhance intestinal epithelial barrier function, modulate host immune responses, and suppress the growth of pathogenic bacteria, yeasts, molds, and viruses. Thus, lactobacilli have been used as probiotics for treating various diseases, including intestinal disorders, and as biological preservatives in the food and agricultural industries. However, the molecular mechanisms used by lactobacilli to suppress pathogenic bacterial infections have been poorly characterized. We previously isolated Lactobacillus plantarum JSA22 from buckwheat sokseongjang, a traditional Korean fermented soybean food, which possessed high enzymatic, fibrinolytic, and broad-spectrum antimicrobial activity against foodborne pathogens. In this study, we investigated the effects of L. plantarum JSA22 on the growth of S. Typhimurium and S. Typhimurium-induced cytotoxicity by stimulating the host immune response in intestinal epithelial cells. The results showed that coincubation of S. Typhimurium and L. plantarum JSA22 with intestinal epithelial cells suppressed S. Typhimurium infection, S. Typhimurium-induced NF-κB activation, and IL-8 production, and lowered the phosphorylation of both Akt and p38. These data indicated that L. plantarum JSA22 has probiotic properties, and can inhibit S. Typhimurium infection of intestinal epithelial cells. Our findings can be used to develop therapeutic and prophylactic agents against pathogenic bacteria.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1299-1309
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• 2018

We previously reported on lactophoricin (LPcin), a cationic ${\alpha}-helical$ antimicrobial peptide derived from bovine milk, which has antimicrobial effects on Candida albicans as well as Gram-positive and Gram-negative bacteria. In this study, we designed the LPcin-YK3 peptide, a shorter analog of LPcin, and investigated its antimicrobial activity. This peptide, consisting of 15 amino acids with + 3 net charges, was an effective antimicrobial agent against the on the Gram-positive strain, Staphylococcus aureus (MIC: $0.62{\mu}g/ml$). In addition, the hemolytic activity assay revealed that the peptide was not toxic to mouse and human erythrocytes up to $40{\mu}g/ml$. We also used circular dichroism spectroscopy to confirm that peptide in the presence of lipid has ${\alpha}-helical$ structures and later provide an overview of the relationship between each structure and antimicrobial activity. This peptide is a member of a new class of antimicrobial agents that could potentially overcome the problem of bacterial resistance caused by overuse of conventional antibiotics. Therefore, it could be used as a therapeutic or natural additive, particularly in the cosmetics industry.

• 한국응용과학기술학회지
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• 제27권4호
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• pp.501-507
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• 2010

In this study, we evaluate the anti-microbiological activity of paraben in eye shadows that are composed of pigments and oil binders using various analytical methods and microbiological tests. Paraben does not show the microbiological activity properly when it was used with Nylon SP$^{(R)}$ 10, Talc RF SSA$^{(R)}$, OMC Talc AS$^{(R)}$ and $BaSO_4$. In the test of fungi, Nylon SP$^{(R)}$ 10 causes the decrease of microbiological activity regardless of the type of oil binders. The pigment of Mango violet also causes the decrease of microbiological activity when ester oil binder was used. Regardless of the type of oil binder, samples containing nylon SP 10, 0.15% of methyl paraben and 0.05% of propyl paraben had not been able to maintain microbiological activity only if the concentration of parabens were increased. Trace amounts of metal ions present in pigments reduced the activity of preservatives by inactivation of hydroxyl group of paraben. It is thought that swollen nylon SP 10 in ester oil increase the absorption or interaction of parabens and swollen nylon powder causes the inactivation of paraben.

• 가정과삶의질연구
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• 제31권4호
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• pp.15-31
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• 2013

Consumers are quite worried about food safety because food is one of the most important necessities in everyday life. Many studies have been conducted regarding the food safety issues, however, most researches have focused only on a limited range of risk factors and used only quantitative survey methods. The purpose of this study was to investigate consumers' perceptions of the risky components of food safety and how the perceptions have been formed. For this study in-depth interviews were conducted. For the interviewees, nine housewives, who are in their 30s to 50s, were selected taking into consideration their age, education level, number of children, and employment status. Results showed that the risk factors many consumers worried about were agricultural pesticides, MSG, food additives, GMO, Mad Cow Disease, preservatives, and growth hormones, etc. Consumers were worried about the risk of the retail process the most because they thought it was not informed and controlled well. Consumers tend to worry much more when the risks are involuntary, uncontrollable, unreliable, and have a gradual negative effect. Some of the food safety-oriented behaviors of consumers were also investigated.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.100-107
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• 2009

Objective : The purpose of this study is to investigate the antibacterial activity of Saussurea lappa for the applications of herb-based extracts to both cosmetic and medicinal industries. Methods : Antibacterial activity of ethanol extract of Saussutes lappa (ESL) was assessed using agar diffusion and broth dilution methods, and determined by whether clear zone was formed around paper disc and in terms of the size (mm) of clear zone, Results : ESL provided activity against Staphylococcus aureus KCTC 1927 in concentration of 1 mg/ml with an clear zone of 16 mm, and showed an activity against Staphylococcus epidermids KCTC 1917 in concentration of 1 mg/ml with an clear zone of 18mm. Moreover, the minimum inhibitory concentration (MIC) of ESL against S. sureus and S. epidermidls were 1 mg/ml for both bacterial species. However, ESL showed no growth inhibition against Pseudomonas aeruginosa KCTC 12513, Enterobactet aerogenes KCTC 2190, Escherichia coli KCTC 2571, Salmonella typhimurium KCTC 1925 and Propionibacterium acnes KCCM 41747. Conclusions : Antibacterial activity of ESL against Staphylococcus sureus and Staphylococcus epidermids causing eye and skin diseases was proved. The result suggest that ESL may be useful as a natural preservative on behalf of synthetic preservatives.